Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,635,384	T→C	E155G (GAA→GGA)	ydfN ←	Qin prophage; putative side tail fiber assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,635,384	0	T	C	100.0%	344.0 / NA	94	E155G (GAA→GGA)	ydfN	Qin prophage; putative side tail fiber assembly protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (49/45);  total (49/45)

TCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATC  >  NC_000913/1635247‑1635518                                                                                                                                          |                                                                                                                                       tCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTccc                                                                                                                                      <  2:943379/140‑1 (MQ=255)  cccGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCt                                                                                                                                     >  1:1234087/1‑140 (MQ=255)    cGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTga                                                                                                                                   >  1:915788/1‑140 (MQ=255)    cGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTga                                                                                                                                   >  1:172294/1‑140 (MQ=255)     gCTAATACTATGGGTATGCGCCTCGGTGTTATTCGTGGATTTGGTTCCGTAAGCAAACGACGATGTGGTTGCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTgag                                                                                                                                  <  2:156073/140‑1 (MQ=255)     gCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTgag                                                                                                                                  <  1:705367/140‑1 (MQ=255)             tATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACg                                                                                                                          >  1:330237/1‑140 (MQ=255)               tGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGc                                                                                                                        >  1:693803/1‑140 (MQ=255)               tGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGc                                                                                                                        <  1:41778/140‑1 (MQ=255)               tGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGc                                                                                                                        >  1:1119006/1‑140 (MQ=255)               tGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGc                                                                                                                        >  2:1258506/1‑140 (MQ=255)               tGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGc                                                                                                                        >  1:1171313/1‑140 (MQ=255)                gggTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGcc                                                                                                                       >  1:512717/1‑140 (MQ=255)                 ggTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGccc                                                                                                                      >  2:1343914/1‑140 (MQ=255)                    aTGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGAc                                                                                                                   >  2:1104125/1‑140 (MQ=255)                     tGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTgaga                                                                                                                                 >  2:23526/1‑125 (MQ=255)                     tGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTgaga                                                                                                                                 <  1:23526/125‑1 (MQ=255)                     tGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGAcc                                                                                                                  >  1:232016/1‑140 (MQ=255)                     tGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGAcc                                                                                                                  >  2:1175702/1‑140 (MQ=255)                     tGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGAcc                                                                                                                  <  2:1234087/140‑1 (MQ=255)                     tGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGAcc                                                                                                                  >  1:347701/1‑140 (MQ=255)                     tGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGAcc                                                                                                                  >  1:242607/1‑140 (MQ=255)                     tGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGAcc                                                                                                                  >  2:1303422/1‑140 (MQ=255)                     tGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGAcc                                                                                                                  >  1:962240/1‑140 (MQ=255)                     tGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGAcc                                                                                                                  >  1:1082380/1‑140 (MQ=255)                     tGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGAcc                                                                                                                  >  2:171442/1‑140 (MQ=255)                      gcgcCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCa                                                                                                                 >  1:29119/1‑140 (MQ=255)                       cgcCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCAc                                                                                                                >  2:654859/1‑140 (MQ=255)                           ccGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGc                                                                                                            >  1:814694/1‑140 (MQ=255)                           ccGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGc                                                                                                            >  2:1135100/1‑140 (MQ=255)                           ccGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGc                                                                                                            <  2:864431/140‑1 (MQ=255)                            cGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCg                                                                                                           >  1:108423/1‑140 (MQ=255)                            cGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCg                                                                                                           >  2:144849/1‑140 (MQ=255)                            cGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCg                                                                                                           >  1:1000822/1‑140 (MQ=255)                               tgtTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGc                                                                                                        <  1:105238/140‑1 (MQ=255)                               tgtTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGc                                                                                                        <  1:97937/140‑1 (MQ=255)                               tgtTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGc                                                                                                        <  1:1205504/140‑1 (MQ=255)                               tgtTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGc                                                                                                        <  1:1104125/140‑1 (MQ=255)                               tgtTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGc                                                                                                        <  2:512717/140‑1 (MQ=255)                               tgtTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGc                                                                                                        <  2:281577/140‑1 (MQ=255)                                 ttATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCtt                                                                                                      >  2:681467/1‑140 (MQ=255)                                 ttATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCtt                                                                                                      <  1:1349410/140‑1 (MQ=255)                                    ttCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGcc                                                                                                   >  2:2076/1‑140 (MQ=255)                                         ggATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTaa                                                                                              >  1:930291/1‑140 (MQ=255)                                         ggATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTaa                                                                                              >  1:958817/1‑140 (MQ=255)                                         ggATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTaa                                                                                              >  2:231965/1‑140 (MQ=255)                                         ggATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTaa                                                                                              >  1:1100253/1‑140 (MQ=255)                                         ggATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTaa                                                                                              >  1:460971/1‑140 (MQ=255)                                             ttGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGt                                                                                          <  1:144849/140‑1 (MQ=255)                                              tGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGTGGGCTTGCCCTTAATCGTc                                                                                         <  2:1020020/140‑1 (MQ=255)                                              tGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTc                                                                                         >  2:775040/1‑140 (MQ=255)                                                gTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCa                                                                                       <  2:330237/140‑1 (MQ=255)                                                 ttCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAg                                                                                      <  1:171442/140‑1 (MQ=255)                                                 ttCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAg                                                                                      <  1:1175702/140‑1 (MQ=255)                                                 ttCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAg                                                                                      <  2:242607/140‑1 (MQ=255)                                                  tCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGc                                                                                     <  2:930291/140‑1 (MQ=255)                                                  tCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGc                                                                                     <  2:232016/140‑1 (MQ=255)                                                      tAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCAcc                                                                                 >  2:1157283/1‑139 (MQ=255)                                                      tAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACg                                                                                 <  1:1135100/140‑1 (MQ=255)                                                      tAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACg                                                                                 <  1:1251128/140‑1 (MQ=255)                                                      tAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACg                                                                                 <  2:1082380/140‑1 (MQ=255)                                                      tAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACg                                                                                 <  2:1000822/140‑1 (MQ=255)                                                      tAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACg                                                                                 <  2:693803/140‑1 (MQ=255)                                                       aaTCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGc                                                                                <  2:958817/140‑1 (MQ=255)                                                        aTCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCa                                                                               <  2:172294/140‑1 (MQ=255)                                                              cgacgaTGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAg                                                                         >  2:1165459/1‑140 (MQ=255)                                                                 cgaTGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGAGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGa                                                                      <  2:327940/140‑1 (MQ=255)                                                                  gaTGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGAt                                                                     <  2:1100253/140‑1 (MQ=255)                                                                        gTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGcc                                                               <  2:460971/140‑1 (MQ=255)                                                                         tttCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCt                                                              <  1:1313911/140‑1 (MQ=255)                                                                          ttCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTg                                                             <  2:1171313/140‑1 (MQ=255)                                                                            ccGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGAc                                                           >  1:559566/1‑140 (MQ=255)                                                                            ccGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGAc                                                           <  2:29119/140‑1 (MQ=255)                                                                            ccGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGAc                                                           <  1:871585/140‑1 (MQ=16)                                                                              gTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACgg                                                         >  1:373972/1‑140 (MQ=255)                                                                               tCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGa                                                        >  2:89985/1‑140 (MQ=255)                                                                                ccccAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGAt                                                       >  1:1090023/1‑140 (MQ=255)                                                                                           tACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCa                                            >  1:1052412/1‑140 (MQ=255)                                                                                           tACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCa                                            <  2:108423/140‑1 (MQ=255)                                                                                              tGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGt                                         <  2:554306/140‑1 (MQ=255)                                                                                                gATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGttt                                       >  1:2718/1‑140 (MQ=255)                                                                                                gATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGttt                                       >  2:553301/1‑140 (MQ=255)                                                                                                  tGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCCGCCCGCCACGCATATCAGGGATCACGCCTGACGGGTAAGCAACTGCAAGTTTCg                                     >  2:274/1‑140 (MQ=14)                                                                                                          cgctgTGGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCa                             >  2:168460/1‑140 (MQ=255)                                                                                                             tgtgGGTGTGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGAt                          <  1:681467/140‑1 (MQ=255)                                                                                                                  gtgtGCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTc                     <  1:231965/140‑1 (MQ=255)                                                                                                                    gtgCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCaa                   >  1:1148806/1‑140 (MQ=255)                                                                                                                    gtgCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCaa                   <  1:521790/140‑1 (MQ=255)                                                                                                                     tgCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCaaa                  >  1:47977/1‑140 (MQ=255)                                                                                                                     tgCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCaaa                  >  1:821729/1‑140 (MQ=255)                                                                                                                     tgCGATTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCaaa                  >  1:1280835/1‑140 (MQ=255)                                                                                                                         aTTTAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTc              <  2:814694/140‑1 (MQ=255)                                                                                                                            tAATGCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGc           <  1:89985/140‑1 (MQ=255)                                                                                                                                gCCGTCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCt       <  1:222095/140‑1 (MQ=255)                                                                                                                                    tCCTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCAt   <  1:1303422/140‑1 (MQ=255)                                                                                                                                     ccTGTCCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATc  <  1:654859/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATC  >  NC_000913/1635247‑1635518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑