Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	232,127	C→T	R68C (CGT→TGT)	yafE →	putative S‑adenosylmethionine‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	232,127	0	C	T	100.0%	289.3 / NA	81	R68C (CGT→TGT)	yafE	putative S‑adenosylmethionine‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/43);  total (38/43)

ACTTATCTGCCCACATGCTGGATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAG  >  NC_000913/231996‑232261                                                                                                                                    |                                                                                                                                       aCTTATCTGCCCACATGCTGGATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCt                                                                                                                                >  2:905178/1‑140 (MQ=255)     aTCTGCCCACATGCTGGATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGccc                                                                                                                            >  1:853603/1‑140 (MQ=255)      tCTGCCCACATGCTGGATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCa                                                                                                                           >  1:625126/1‑140 (MQ=255)       cTGCCCACATGCTGGATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCAt                                                                                                                          <  2:625126/140‑1 (MQ=255)         gCCCACATGCTGGATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCa                                                                                                                        <  1:1161794/140‑1 (MQ=255)          cccACATGCTGGATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCAc                                                                                                                       <  1:904396/140‑1 (MQ=255)          cccACATGCTGGATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCAc                                                                                                                       >  2:86882/1‑140 (MQ=255)                tGCTGGATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCat                                                                                                                 <  2:579424/140‑1 (MQ=255)                    ggATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCa                                                                                                                        >  2:362773/1‑129 (MQ=255)                    ggATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCa                                                                                                                        >  2:550003/1‑129 (MQ=255)                    ggATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCa                                                                                                                        <  1:362773/129‑1 (MQ=255)                    ggATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCa                                                                                                                        <  1:550003/129‑1 (MQ=255)                    ggATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCatgatg                                                                                                             >  2:307028/1‑140 (MQ=255)                     gATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGt                                                                                                            <  2:165352/140‑1 (MQ=255)                     gATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGt                                                                                                            >  2:1029280/1‑140 (MQ=255)                         tCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGt                                                                                                        >  1:732311/1‑140 (MQ=255)                         tCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGt                                                                                                        >  2:822866/1‑140 (MQ=255)                          cGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTg                                                                                                       >  2:514033/1‑140 (MQ=255)                          cGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTg                                                                                                       <  1:514033/140‑1 (MQ=255)                            tGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGt                                                                                                        <  2:952941/137‑1 (MQ=255)                            tGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGt                                                                                                        >  1:952941/1‑137 (MQ=255)                               cacaAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGt                                                                                                            <  1:651324/130‑1 (MQ=255)                               cacaAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGt                                                                                                            >  2:651324/1‑130 (MQ=255)                                   aGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGc                                                                                              >  2:443312/1‑140 (MQ=255)                                    gCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCCGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCg                                                                                             >  2:893243/1‑140 (MQ=255)                                      tGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCgag                                                                                           <  1:905178/140‑1 (MQ=255)                                      tGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCga                                                                                            >  1:312144/1‑139 (MQ=255)                                      tGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCga                                                                                            <  2:312144/139‑1 (MQ=255)                                        ccGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGaa                                                                                         <  1:86882/140‑1 (MQ=255)                                        ccGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGaa                                                                                         <  1:1124279/140‑1 (MQ=255)                                          gAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGt                                                                                       <  1:406225/140‑1 (MQ=255)                                          gAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGt                                                                                       <  1:911139/140‑1 (MQ=255)                                            aGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGa                                                                                     <  1:443312/140‑1 (MQ=255)                                               ccGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGaa                                                                                    >  1:244829/1‑138 (MQ=255)                                               ccGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGaa                                                                                    <  2:244829/138‑1 (MQ=255)                                               ccGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATa                                                                                  <  1:494278/140‑1 (MQ=255)                                                 ggCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAgg                                                                                <  1:1265975/140‑1 (MQ=255)                                                  gCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGa                                                                               >  1:1338095/1‑140 (MQ=255)                                                       tGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTg                                                                          >  2:922893/1‑140 (MQ=255)                                                       tGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTg                                                                          >  1:66629/1‑140 (MQ=255)                                                                  accaccCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCgg                                                               <  1:922893/140‑1 (MQ=255)                                                                  accaccCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCgg                                                               <  2:66629/140‑1 (MQ=255)                                                                   ccaccCGCCAGGAATATGCCGAAAGTCTGCCATTTACCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGt                                                              >  2:381743/1‑140 (MQ=255)                                                                      cccGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAgg                                                           >  1:5050/1‑140 (MQ=255)                                                                      cccGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAgg                                                           <  2:1064982/140‑1 (MQ=255)                                                                        cGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCt                                                         >  2:597113/1‑140 (MQ=255)                                                                        cGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCt                                                         >  2:982011/1‑140 (MQ=255)                                                                        cGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCt                                                         >  2:128274/1‑140 (MQ=255)                                                                               gATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATtgtg                                                  <  1:982011/140‑1 (MQ=255)                                                                                atatGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGa                                                 <  1:1210363/140‑1 (MQ=255)                                                                                 tatGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGAt                                                >  2:584137/1‑140 (MQ=255)                                                                                  atGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATg                                               >  2:824208/1‑140 (MQ=255)                                                                                  atGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATg                                               <  2:5050/140‑1 (MQ=255)                                                                                   tGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATgg                                              <  1:1026993/140‑1 (MQ=255)                                                                                        aaaGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTa                                         <  1:128274/140‑1 (MQ=255)                                                                                        aaaGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTa                                         >  1:69071/1‑140 (MQ=255)                                                                                        aaaGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTa                                         <  1:597113/140‑1 (MQ=255)                                                                                             cTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGAt                                                      >  2:146605/1‑122 (MQ=255)                                                                                             cTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGAt                                                      <  1:146605/122‑1 (MQ=255)                                                                                              tGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGtct                                   <  2:728412/140‑1 (MQ=255)                                                                                              tGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGtct                                   >  2:898801/1‑140 (MQ=255)                                                                                              tGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGtct                                   <  2:732311/140‑1 (MQ=255)                                                                                              cgCCATTTGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGtct                                   <  1:893243/139‑1 (MQ=255)                                                                                                   tttGCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCggg                              <  1:824208/140‑1 (MQ=255)                                                                                                      gCCGATAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCa                           >  1:1271424/1‑140 (MQ=255)                                                                                                          aTAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCgg                               <  2:835059/132‑1 (MQ=255)                                                                                                          aTAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCa                       >  2:427016/1‑140 (MQ=255)                                                                                                          aTAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGAGATGGACGTAATGTCTCCgg                               >  1:835059/1‑132 (MQ=255)                                                                                                           tAACGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAg                      <  1:427016/140‑1 (MQ=255)                                                                                                            aaCGCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGt                     >  1:1039462/1‑140 (MQ=255)                                                                                                               gCATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTgcg                  <  2:1271424/140‑1 (MQ=255)                                                                                                                cATTTGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTgcgc                 <  1:381743/140‑1 (MQ=255)                                                                                                                  tttGATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGa               <  2:655526/140‑1 (MQ=255)                                                                                                                     gATATTGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACAt            <  2:69071/140‑1 (MQ=255)                                                                                                                         ttGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGa               >  1:747889/1‑133 (MQ=255)                                                                                                                         ttGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGa               <  2:747889/133‑1 (MQ=255)                                                                                                                         ttGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACAt            >  2:758715/1‑136 (MQ=255)                                                                                                                         ttGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACAt            <  1:758715/136‑1 (MQ=255)                                                                                                                            ttATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTa     >  1:5379/1‑140 (MQ=255)                                                                                                                               tCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAg  <  1:695068/140‑1 (MQ=255)                                                                                                                               tCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAg  >  2:695068/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                       ACTTATCTGCCCACATGCTGGATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAG  >  NC_000913/231996‑232261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑