Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,933,347	T→C	T420A (ACA→GCA)	edd ←	phosphogluconate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,933,347	0	T	C	100.0%	170.9 / NA	47	T420A (ACA→GCA)	edd	phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/17);  total (30/17)

ACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACC  >  NC_000913/1933213‑1933476                                                                                                                                       |                                                                                                                                  aCCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACTCTTTTGCCccac                                                                                                                              >  2:1238456/1‑140 (MQ=255) aCCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCccac                                                                                                                              >  1:1179051/1‑140 (MQ=255)  cccGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCccacc                                                                                                                             <  1:567669/140‑1 (MQ=255)   ccGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCccacca                                                                                                                            >  1:829609/1‑140 (MQ=255)   ccGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCccacca                                                                                                                            >  1:706391/1‑140 (MQ=255)   ccGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCccacca                                                                                                                            <  2:531256/140‑1 (MQ=255)     gCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatg                                                                                                                          >  1:853781/1‑140 (MQ=255)       ttCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgat                                                                                                                        <  2:1039676/140‑1 (MQ=255)       ttCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgat                                                                                                                        <  1:1171614/140‑1 (MQ=255)       ttCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgat                                                                                                                        <  1:889552/140‑1 (MQ=255)       ttCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgat                                                                                                                        <  1:1333724/140‑1 (MQ=255)       ttCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgat                                                                                                                        >  1:783779/1‑140 (MQ=255)       ttCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgat                                                                                                                        <  2:1361821/140‑1 (MQ=255)        tCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgatg                                                                                                                       >  1:1135213/1‑140 (MQ=255)            aGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATgagag                                                                                                                   <  1:411807/140‑1 (MQ=255)            aGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATgagag                                                                                                                   <  1:674538/140‑1 (MQ=255)                 ggCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAgg                                                                                                              >  1:68720/1‑140 (MQ=255)                         gTCATGCTGCTTTTCAAAATCAACCGCTGGCGCTTCAATCTCCTGGGTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACGCTTTTGCCCCTCCATGATGAGAGAAAGGTTGTTCga                                                                                                      <  2:829609/140‑1 (MQ=255)                            aTGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCgaagg                                                                                                   >  2:1316293/1‑140 (MQ=255)                             tGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCgaagga                                                                                                  <  2:853781/140‑1 (MQ=255)                                tGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACTCTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGc                                                                                               <  1:1238456/140‑1 (MQ=255)                                tGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGc                                                                                               <  1:1302626/140‑1 (MQ=255)                                  gCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGa                                                                                             >  1:257921/1‑140 (MQ=255)                                   cTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGAt                                                                                            <  2:68720/140‑1 (MQ=255)                                   cTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGAt                                                                                            >  1:808317/1‑140 (MQ=255)                                     ttCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATca                                                                                          <  2:808317/140‑1 (MQ=255)                                              aacCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGt                                                                                 >  1:1148730/1‑140 (MQ=255)                                                ccGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCg                                                                               >  2:1288953/1‑140 (MQ=255)                                                                  ccTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGc                                                                 >  1:1046031/1‑136 (MQ=255)                                                                  ccTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGc                                                                 <  2:68030/136‑1 (MQ=255)                                                                  ccTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGc                                                                 <  2:1046031/136‑1 (MQ=255)                                                                  ccTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGc                                                                 >  1:68030/1‑136 (MQ=255)                                                                   cTGTTTCTCAACCGGCACGGCAGAGTTTTTCATACCCGCCCGGCCCAGGTTACCGCTTAACCCTTTTCCCCCACCATGATGAGAAAAGGGTTGTTCGAAGGAAGCGATCACATTGCTGTGGAGTGTTTTTCCCGCCCCtt                                                            <  2:257921/140‑1 (MQ=255)                                                                    tGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTc                                                           >  1:232737/1‑140 (MQ=255)                                                                        tctcACCCGGCACGGCAGAGGTTTTCATACCCGCACGGCCAAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAAAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGTTTTTTCCGCCCCTTCCCGc                                                       <  2:485601/140‑1 (MQ=255)                                                                         ctcAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGcc                                                      >  2:2762/1‑140 (MQ=255)                                                                             aCCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGccagtc                                                  >  2:988657/1‑140 (MQ=255)                                                                                 gcacggcaGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGccagtccagt                                              >  1:602979/1‑140 (MQ=255)                                                                                 gcacggcaGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGccagtccagt                                              >  2:522494/1‑140 (MQ=255)                                                                                  cacggcaGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAAAAAGTTTGTCCGAAGGAAGCGATCACATTGCTGTGGAGTGTTTTTTCCGCCCCTTCCCGCCAGTCCAGtt                                             <  2:706391/140‑1 (MQ=255)                                                                                            tttttCATACCCGCACGGCCCAGGTTACCGCTTAACCTTTTTCCCCCACCATGATGAGAGAAGGGTTGTTCGAGGGAAGCGATCACTTTGCTGTCGAGTGTTTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCattatt                                   <  2:1135213/139‑1 (MQ=255)                                                                                                 cATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGTTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGTTTTTTCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGcc                              <  2:1179051/140‑1 (MQ=255)                                                                                                      ccGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGt                         >  1:1006215/1‑140 (MQ=255)                                                                                                         cACGGCCCGGGTTACCGCTTAACATTTTTCCCCCCCCATGATGAGAGAAAGTTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTTTTTTTTCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCa                      <  2:602979/140‑1 (MQ=255)                                                                                                         cACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCa                      >  2:449900/1‑140 (MQ=255)                                                                                                         cACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCa                      >  1:1160752/1‑140 (MQ=255)                                                                                                             gCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGTTTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAggg                  <  2:1148730/140‑1 (MQ=255)                                                                                                                  ggTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATaa             >  1:1281134/1‑140 (MQ=255)                                                                                                                        cGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCTAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac       >  2:634640/1‑140 (MQ=255)                                                                                                                        cGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac       >  1:582102/1‑140 (MQ=255)                                                                                                                        cGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac       >  2:24459/1‑140 (MQ=255)                                                                                                                        cGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac       >  2:728989/1‑140 (MQ=255)                                                                                                                        cGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac       >  2:759353/1‑140 (MQ=255)                                                                                                                             aaCACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGAcc  >  1:1354269/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                  ACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACC  >  NC_000913/1933213‑1933476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑