Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,064,847	G→A	L126L (CTG→TTG)	cobS ←	cobalamin 5'‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,064,847	0	G	A	100.0%	192.4 / NA	54	L126L (CTG→TTG)	cobS	cobalamin 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/23);  total (31/23)

TGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCA  >  NC_000913/2064715‑2064981                                                                                                                                     |                                                                                                                                       tGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCttt                                                                                                                                 >  2:543885/1‑140 (MQ=255)   ccAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGc                                                                                                                               <  1:167129/140‑1 (MQ=255)   ccAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGc                                                                                                                               <  1:973062/140‑1 (MQ=255)        cccTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTa                                                                                                                          <  2:1324674/140‑1 (MQ=255)        cccTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTa                                                                                                                          <  1:885638/140‑1 (MQ=255)         ccTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTAc                                                                                                                         <  1:1294623/140‑1 (MQ=255)             cttcCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACcaca                                                                                                                     >  2:915482/1‑140 (MQ=255)             cttcCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACcaca                                                                                                                     <  2:402648/140‑1 (MQ=255)             cttcCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACcaca                                                                                                                     >  1:60317/1‑140 (MQ=255)             cttcCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACcaca                                                                                                                     >  2:1343812/1‑140 (MQ=255)                  cgcgCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAAt                                                                                                                <  1:963259/140‑1 (MQ=255)                     gcATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACGCGCTTAACACCAAGATCTTTGCCAGTACCACAAAGATCaa                                                                                                             >  2:1208224/1‑140 (MQ=255)                            gATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGa                                                                                                      <  1:61425/140‑1 (MQ=255)                                 cGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGAccgcc                                                                                                 >  2:913326/1‑140 (MQ=255)                                     aCATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTgg                                                                                             >  2:941191/1‑140 (MQ=255)                                            taatGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTa                                                                                      >  1:617370/1‑140 (MQ=255)                                              atGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTaaa                                                                                    >  2:265199/1‑140 (MQ=255)                                               tGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAAc                                                                                   >  2:1000043/1‑140 (MQ=255)                                               tGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAAc                                                                                   >  1:938284/1‑140 (MQ=255)                                               tGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAAc                                                                                   >  1:1147475/1‑140 (MQ=255)                                                 cGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACgg                                                                                 <  2:617370/140‑1 (MQ=255)                                                 cGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACgg                                                                                 >  2:172128/1‑140 (MQ=255)                                                 cGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACgg                                                                                 >  1:37841/1‑140 (MQ=255)                                                 cGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACgg                                                                                 >  1:1016363/1‑140 (MQ=255)                                                   gCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCt                                                                               >  2:563673/1‑140 (MQ=255)                                                     aGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTAt                                                                             <  1:913326/140‑1 (MQ=255)                                                      gTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATc                                                                            >  1:625501/1‑140 (MQ=255)                                                       tCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCa                                                                           >  2:760431/1‑140 (MQ=255)                                                           gcgGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCa                                                                       <  2:908601/140‑1 (MQ=255)                                                           gcgGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCa                                                                       <  1:760431/140‑1 (MQ=255)                                                                 gACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGAttt                                                                 >  1:203689/1‑140 (MQ=255)                                                                  aCCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTc                                                                >  2:809145/1‑140 (MQ=255)                                                                                 gccAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATcgcg                                                 >  2:343855/1‑140 (MQ=255)                                                                                     gCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTa                                             >  1:904010/1‑140 (MQ=255)                                                                                     gCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTa                                             <  2:625501/140‑1 (MQ=255)                                                                                                aTCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGaaaa                                  >  1:451137/1‑140 (MQ=255)                                                                                                   gATTCGCCACGTAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATAc                               <  2:904010/140‑1 (MQ=255)                                                                                                    aTTCGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACg                              >  1:846967/1‑140 (MQ=255)                                                                                                       cGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCa                           <  1:1000043/140‑1 (MQ=255)                                                                                                       cGCCACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCa                           <  2:203689/140‑1 (MQ=255)                                                                                                          cACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATca                        >  1:219441/1‑140 (MQ=255)                                                                                                          cACGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATca                        <  1:1343812/140‑1 (MQ=255)                                                                                                           aCGCAGAGCCAACTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATcac                       <  1:450472/140‑1 (MQ=255)                                                                                                                      aCTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAg                     >  2:257313/1‑131 (MQ=255)                                                                                                                      aCTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAg                     <  1:257313/131‑1 (MQ=255)                                                                                                                       cTCGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGa           >  1:926225/1‑140 (MQ=255)                                                                                                                         cGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAgg         <  1:172128/140‑1 (MQ=255)                                                                                                                         cGCTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAgg         <  1:543885/140‑1 (MQ=255)                                                                                                                           cTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATc                         <  1:641071/122‑1 (MQ=255)                                                                                                                           cTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATc                         >  2:641071/1‑122 (MQ=255)                                                                                                                           cTTAACACCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGcc       >  1:464031/1‑140 (MQ=255)                                                                                                                               acacCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTcc   <  1:1282731/140‑1 (MQ=255)                                                                                                                                cacCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCa  >  2:77401/1‑140 (MQ=255)                                                                                                                                cacCAAAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCa  >  2:208141/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       TGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCA  >  NC_000913/2064715‑2064981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑