Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,155,225	A→G	T404A (ACC→GCC)	mdtA →	multidrug efflux pump membrane fusion protein MdtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,155,225	0	A	G	100.0%	225.2 / NA	62	T404A (ACC→GCC)	mdtA	multidrug efflux pump membrane fusion protein MdtA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/33);  total (29/33)

GAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGC  >  NC_000913/2155095‑2155360                                                                                                                                   |                                                                                                                                        gAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCcgcg                                                                                                                                >  1:504092/1‑140 (MQ=255)        gtgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATAcgc                                                                                                                         >  2:151960/1‑140 (MQ=255)        gtgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGAT‑‑CCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGa                                                                                                                       >  1:1053636/1‑140 (MQ=255)                tGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAg                                                                                                                 >  2:939502/1‑140 (MQ=255)                tGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAg                                                                                                                 >  2:574218/1‑140 (MQ=255)                tGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGAT‑‑CCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGa                                                                                                               <  2:1053636/140‑1 (MQ=255)                     gTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCa                                                                                                            <  1:650592/140‑1 (MQ=255)                     gTATTTCTGCGGGCGATCGCATGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCa                                                                                                            <  2:1102575/140‑1 (MQ=255)                      tATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCAc                                                                                                           <  1:998398/140‑1 (MQ=255)                       aTTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACg                                                                                                          <  2:60583/140‑1 (MQ=255)                        ttgcTGCGGGCGATGGCGTTGTGACAGACGGCATTGACCGCCTGACCGAAGGGGCGAAAGTTGAAGTGGTGGAACCCCAGAGCGCCACTACTCCGGAAGATAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCAcac                                                                                                         <  2:1357158/137‑3 (MQ=255)                         ttCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCt                                                                                                        >  2:846945/1‑140 (MQ=255)                         ttCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCt                                                                                                        <  1:846945/140‑1 (MQ=255)                         ttCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCt                                                                                                        >  2:58446/1‑140 (MQ=255)                         ttCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCt                                                                                                        <  1:58446/140‑1 (MQ=255)                          tCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTc                                                                                                       >  1:434412/1‑140 (MQ=255)                               gggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGAt                                                                                                  >  2:1072570/1‑140 (MQ=255)                               gggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGAt                                                                                                  >  1:397709/1‑140 (MQ=255)                               gggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGAt                                                                                                  >  1:959592/1‑140 (MQ=255)                                ggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATg                                                                                                 <  1:1198445/140‑1 (MQ=255)                                ggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATg                                                                                                 <  1:1115199/140‑1 (MQ=255)                                ggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATg                                                                                                 >  1:598571/1‑140 (MQ=255)                                 gCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGc                                                                                                <  1:433245/140‑1 (MQ=255)                                  cGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCa                                                                                               <  2:397709/140‑1 (MQ=255)                                  cGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCa                                                                                               <  2:787132/140‑1 (MQ=255)                                  cGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCa                                                                                               <  1:956517/140‑1 (MQ=255)                                      cgcgTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGgtg                                                                                           >  1:427599/1‑140 (MQ=255)                                       gcgTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGgtgt                                                                                          <  2:504092/140‑1 (MQ=255)                                       gcgTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGgtgt                                                                                          <  1:896723/140‑1 (MQ=255)                                       gcgTGAGGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGACCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGgtgt                                                                                          <  2:318347/140‑1 (MQ=255)                                            gtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTAccc                                                                                     <  2:598571/140‑1 (MQ=255)                                            gtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTAccc                                                                                     <  1:1011327/140‑1 (MQ=255)                                                   aCGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGagca                                                                              >  2:473192/1‑140 (MQ=255)                                                           gATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAggc                                                                      >  2:143623/1‑140 (MQ=255)                                                             tCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAggcgg                                                                    >  1:902979/1‑140 (MQ=255)                                                                 cTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCg                                                                >  2:512712/1‑140 (MQ=255)                                                                 cTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCg                                                                >  1:205962/1‑140 (MQ=255)                                                                 cTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCg                                                                >  2:437728/1‑140 (MQ=255)                                                                  tGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGt                                                               <  2:434412/140‑1 (MQ=255)                                                                      cGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCCCTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTcgcg                                                           <  2:902979/140‑1 (MQ=255)                                                                      cGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTcgcg                                                           <  2:555232/140‑1 (MQ=255)                                                                      cGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTcgcg                                                           <  2:1112700/140‑1 (MQ=255)                                                                        aaGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTcgcg                                                           >  2:1223631/1‑138 (MQ=255)                                                                        aaGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTcgcg                                                           <  1:1223631/138‑1 (MQ=255)                                                                        aaGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGcc                                                         <  1:151960/140‑1 (MQ=255)                                                                        aaGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGcc                                                         <  1:574218/140‑1 (MQ=255)                                                                           gggCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGt                                                      >  2:107499/1‑140 (MQ=255)                                                                                   gtggaagtggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATg                                              <  2:427599/140‑1 (MQ=255)                                                                                      gaagtggTGGAAGCCCAGAGCGCCACTACTCCGGAAGTGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCGGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGt                                           >  2:1207164/1‑140 (MQ=255)                                                                                       aagtggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTc                                          >  2:430475/1‑140 (MQ=255)                                                                                       aagtggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTc                                          <  1:939502/140‑1 (MQ=255)                                                                                                 aGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGccac                                <  1:430475/140‑1 (MQ=255)                                                                                                 aGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGccac                                <  1:437728/140‑1 (MQ=255)                                                                                                     cAGAGCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACg                            >  2:807730/1‑140 (MQ=255)                                                                                                        agCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACgctg                         <  2:426122/140‑1 (MQ=255)                                                                                                        agCGCCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACgctg                         >  2:871859/1‑140 (MQ=255)                                                                                                           gcCACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGctgctg                      <  2:205962/140‑1 (MQ=255)                                                                                                            ccACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGa                     <  1:143623/140‑1 (MQ=255)                                                                                                            ccACTACTCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGa                     <  1:807730/140‑1 (MQ=255)                                                                                                                tactCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGAtggt                 <  1:512712/140‑1 (MQ=255)                                                                                                                  ctCCGGAAGAGAAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGAtggtgg               >  1:1334872/1‑140 (MQ=255)                                                                                                                            gaAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACt     >  2:1300815/1‑140 (MQ=255)                                                                                                                            gaAAGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACt     >  2:268334/1‑140 (MQ=255)                                                                                                                              aaGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCg   <  1:473192/140‑1 (MQ=255)                                                                                                                               aGCCGCCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGc  >  1:976377/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        GAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGC  >  NC_000913/2155095‑2155360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑