Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,380,598	A→G	S16P (TCG→CCG)	menF ←	isochorismate synthase MenF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,380,598	0	A	G	100.0%	321.6 / NA	87	S16P (TCG→CCG)	menF	isochorismate synthase MenF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/44);  total (43/44)

CCATTACGTTGTTGCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGACAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTACCCCGGTGTAGGGAGGTTTTAACGGCGTGCC  >  NC_000913/2380465‑2380734                                                                                                                                      |                                                                                                                                         ccATTACGTTGTTGCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATg                                                                                                                                    >  2:729432/1‑140 (MQ=255) ccATTACGTTGTTGCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATg                                                                                                                                    >  2:392280/1‑140 (MQ=255)    ttACGTTGTTGCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGgcg                                                                                                                                 >  2:401946/1‑140 (MQ=255)    ttACGTTGTTGCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGgcg                                                                                                                                 >  1:735629/1‑140 (MQ=255)     tACGTTGTTGCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGgcgc                                                                                                                                >  2:67820/1‑140 (MQ=255)            ttgCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCa                                                                                                                               >  2:293389/1‑134 (MQ=255)            ttgCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCa                                                                                                                               <  1:293389/134‑1 (MQ=255)            ttgCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGAt                                                                                                                         >  1:539680/1‑140 (MQ=255)            ttgCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGAt                                                                                                                         >  1:610757/1‑140 (MQ=255)            ttgCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGAt                                                                                                                         >  1:252922/1‑140 (MQ=255)            ttgCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGAt                                                                                                                         >  1:1168727/1‑140 (MQ=255)              gCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGAttt                                                                                                                       >  1:597311/1‑140 (MQ=255)                 aGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTcc                                                                                                                    >  1:694569/1‑140 (MQ=255)                 aGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTcc                                                                                                                    >  1:933896/1‑140 (MQ=255)                   tAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAg                                                                                                                  >  1:219203/1‑140 (MQ=255)                   tAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAg                                                                                                                  <  1:300373/140‑1 (MQ=255)                   tAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAg                                                                                                                  >  2:656305/1‑140 (MQ=255)                    aGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAgc                                                                                                                 <  1:820459/140‑1 (MQ=255)                    aGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAgc                                                                                                                 <  1:37353/140‑1 (MQ=255)                      aaTTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAgcgc                                                                                                               <  2:933896/140‑1 (MQ=255)                      aaTTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAgcgc                                                                                                               <  2:1152272/140‑1 (MQ=255)                      aaTTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAgcgc                                                                                                               <  2:252922/140‑1 (MQ=255)                      aaTTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAgcgc                                                                                                               <  1:392280/140‑1 (MQ=255)                        ttGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCg                                                                                                             <  2:1267320/140‑1 (MQ=255)                        ttGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCg                                                                                                             <  1:376884/140‑1 (MQ=255)                        ttGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCg                                                                                                             <  1:67820/140‑1 (MQ=255)                             ggTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCgtagta                                                                                                        >  1:1365935/1‑140 (MQ=255)                               tATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCg                                                                                                             <  1:949965/133‑1 (MQ=255)                               tATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCg                                                                                                             >  2:949965/1‑133 (MQ=255)                                atttttGCTGACTGGCCAGCCAGCTCAAGGCATCGAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCgtagta                                                                                                        >  2:1210748/4‑137 (MQ=255)                                atttttGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCgtagta                                                                                                        <  1:1210748/134‑1 (MQ=255)                                   tttGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGAt                                                                                                  <  2:539680/140‑1 (MQ=255)                                        tGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCAc                                                                                             >  2:910211/1‑140 (MQ=255)                                         gACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCAcg                                                                                            <  2:610757/140‑1 (MQ=255)                                         gACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCAcg                                                                                            <  2:694569/140‑1 (MQ=255)                                          aCTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCAcgc                                                                                           >  2:77220/1‑140 (MQ=255)                                           cTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCAcgcg                                                                                          <  1:535414/140‑1 (MQ=255)                                           cTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTAAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCAcgcg                                                                                          >  2:358984/1‑140 (MQ=255)                                              gccagccaGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGAc                                                                                       <  2:655311/140‑1 (MQ=255)                                                  gccagcTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACctct                                                                                   <  1:77220/140‑1 (MQ=255)                                                  gccagcTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACctct                                                                                   <  1:1365140/140‑1 (MQ=255)                                                  gccagcTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACctct                                                                                   <  2:1168727/140‑1 (MQ=255)                                                             gCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTccc                                                                                >  1:1367274/1‑132 (MQ=255)                                                             gCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTccc                                                                                <  2:1367274/132‑1 (MQ=255)                                                             gCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTaaaa                                                                        >  2:1269051/1‑140 (MQ=255)                                                               aTCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGc                                                                      >  1:192361/1‑140 (MQ=255)                                                                tCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGcc                                                                     <  1:811254/140‑1 (MQ=255)                                                                tCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGcc                                                                     <  2:1365935/140‑1 (MQ=255)                                                                tCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGcc                                                                     <  2:597311/140‑1 (MQ=255)                                                                tCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGcc                                                                     <  2:1253627/140‑1 (MQ=255)                                                                 cAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTcc                                                                                 >  2:482713/1‑127 (MQ=255)                                                                 cAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTcc                                                                                 <  1:482713/127‑1 (MQ=255)                                                                 cAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGcc                                                                     <  2:384129/139‑1 (MQ=255)                                                                 cAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGcc                                                                     >  1:384129/1‑139 (MQ=255)                                                                 cAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCt                                                                    >  2:302101/1‑140 (MQ=255)                                                                 cAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCt                                                                    >  2:77972/1‑140 (MQ=255)                                                                   aaaGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTca                                                                  <  2:192361/140‑1 (MQ=255)                                                                       cGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGcc                                                                     >  1:1035573/1‑133 (MQ=255)                                                                       cGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGcc                                                                     <  2:1035573/133‑1 (MQ=255)                                                                       cGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACAtt                                                              >  2:869546/1‑140 (MQ=255)                                                                          cTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATtata                                                           <  1:869546/140‑1 (MQ=255)                                                                           tttGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATAc                                                          >  2:963616/1‑140 (MQ=255)                                                                                gagGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGt                                                     <  1:910211/140‑1 (MQ=255)                                                                                  gggAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGtac                                                   >  1:638510/1‑140 (MQ=255)                                                                                   ggAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTc                                                                   >  1:638194/1‑123 (MQ=255)                                                                                   ggAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTc                                                                   <  2:638194/123‑1 (MQ=255)                                                                                    gAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGtacta                                                 >  1:347744/1‑140 (MQ=255)                                                                                    gAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGGAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGtacta                                                 >  1:256528/1‑140 (MQ=255)                                                                                             atCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACaaaaaaaa                                        >  2:959719/1‑140 (MQ=255)                                                                                               cGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATg                                      <  2:735629/140‑1 (MQ=255)                                                                                                 aTAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCa                                    >  2:1284211/1‑140 (MQ=255)                                                                                                  tAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAg                                   <  1:380902/140‑1 (MQ=255)                                                                                                     cccGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTAc                                >  1:469485/1‑140 (MQ=255)                                                                                                      ccGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTAcc                               <  2:256528/140‑1 (MQ=255)                                                                                                      ccGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTAcc                               <  1:1284211/140‑1 (MQ=255)                                                                                                      ccGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTAcc                               <  1:358984/140‑1 (MQ=255)                                                                                                      ccGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTAcc                               <  1:963616/140‑1 (MQ=255)                                                                                                       cGAATGCCGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTAccc                              <  2:469485/140‑1 (MQ=255)                                                                                                              cGGGTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTACCCCGGTGTa                       >  1:38896/1‑140 (MQ=255)                                                                                                                ggTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTACCCCGgt                          >  1:1143591/1‑135 (MQ=255)                                                                                                                ggTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTACCCCGgt                          <  2:1143591/135‑1 (MQ=255)                                                                                                                ggTGTCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTACCCCGGTGTAgg                     >  1:1198751/1‑140 (MQ=255)                                                                                                                 gtgtCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTACCCCGGTGTAggg                    <  2:347744/140‑1 (MQ=255)                                                                                                                  tgtCGCCGGAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTACCCCGGTGTAGGGa                   <  1:77972/140‑1 (MQ=255)                                                                                                                          gAATCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTACCCCGGTGTAGGGAGGTTTTaa           >  1:426788/1‑140 (MQ=255)                                                                                                                            aTCTCTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTACCCCGGTGTAGGGAGGTTTTAACg         >  2:115518/1‑140 (MQ=255)                                                                                                                             tctcTTGCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTACCCCGGTGTAGGGAGGTTTTAACgg        <  2:219203/140‑1 (MQ=255)                                                                                                                                   gCGGCAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTACCCCGGTGTAGGGAGGTTTTAACGGCGTGcc  <  2:996874/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CCATTACGTTGTTGCCAGTAGAATTGCGGGTATGTTTGCTGACTGGCCAGCCAGCTCAAGGCATCAAAAGCGTCTTTGAGAGGGAAAGGAATATCGATAACCCGAATGCCGGGTGTCGCCGGAATCTCTTGCGACAAATGGCGCAGTAGATTTTCCAGCGCCGTAGTAAGTGATTGCACGCGGACCTCTCCCTGTTAAAAGCCTCACATTATACGGGGTACTACAAAAAAAATGCAGTACCCCGGTGTAGGGAGGTTTTAACGGCGTGCC  >  NC_000913/2380465‑2380734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑