Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,566,179	T→C	T236A (ACG→GCG)	eutA ←	reactivating factor for ethanolamine ammonia‑lyase EutBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,566,179	0	T	C	100.0%	166.9 / NA	47	T236A (ACG→GCG)	eutA	reactivating factor for ethanolamine ammonia‑lyase EutBC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/25);  total (22/25)

GCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTA  >  NC_000913/2566046‑2566306                                                                                                                                      |                                                                                                                                gCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTc                                                                                                                           >  1:703939/1‑140 (MQ=255) gCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTc                                                                                                                           >  1:200898/1‑140 (MQ=255) gCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTc                                                                                                                           >  2:390269/1‑140 (MQ=255)        aGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACt                                                                                                                    <  2:703939/140‑1 (MQ=255)          aaCGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTc                                                                                                                  >  1:565192/1‑140 (MQ=255)               gTCGGCGGGCTGGTGGCGATAACATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACaa                                                                                                             >  2:677521/1‑140 (MQ=255)                         tggtggCGATAACATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGc                                                                                                   >  2:256499/1‑140 (MQ=255)                          ggtggCGATAACATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGcc                                                                                                  <  2:8970/140‑1 (MQ=255)                                gATAACATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATccgc                                                                                            <  2:787323/140‑1 (MQ=255)                                   aaCATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCgg                                                                                         <  2:565192/140‑1 (MQ=255)                                    aCATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCggg                                                                                        <  1:331508/140‑1 (MQ=255)                                      aTTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTc                                                                                      >  1:724111/1‑140 (MQ=255)                                               cgccgcCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCAcc                                                                             >  1:242366/1‑140 (MQ=255)                                               cgccgcCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCAcc                                                                             >  1:934387/1‑140 (MQ=255)                                                gccgccAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCa                                                                                     <  2:173472/131‑1 (MQ=255)                                                gccgccAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCa                                                                                     >  1:173472/1‑131 (MQ=255)                                                            cGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCgg                                                                >  1:971422/1‑140 (MQ=255)                                                             gTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGt                                                               <  2:335329/140‑1 (MQ=255)                                                                      tCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTc                                                              <  1:257249/132‑1 (MQ=255)                                                                      tCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTc                                                              >  2:257249/1‑132 (MQ=255)                                                                      tCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACg                                                      >  1:372990/1‑140 (MQ=255)                                                                       cGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACgg                                                     <  2:934387/140‑1 (MQ=255)                                                                            gTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTc                                                <  2:1067670/140‑1 (MQ=255)                                                                            gTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTc                                                <  2:372990/140‑1 (MQ=255)                                                                            gTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTc                                                <  1:677521/140‑1 (MQ=255)                                                                            gTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTc                                                <  2:200898/140‑1 (MQ=255)                                                                                 acCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGc                                           <  2:952495/140‑1 (MQ=255)                                                                                 acCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGc                                           <  1:390269/140‑1 (MQ=255)                                                                                      cGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCa                                      >  2:788634/1‑140 (MQ=255)                                                                                       gggCTGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCAc                                     <  2:1257248/140‑1 (MQ=255)                                                                                        ggCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTc                                                <  1:509314/128‑1 (MQ=255)                                                                                        ggCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTc                                                >  2:509314/1‑128 (MQ=255)                                                                                         gcagcaAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCg                                   <  1:256499/140‑1 (MQ=255)                                                                                           agcaAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGaa                                 >  1:384933/1‑140 (MQ=255)                                                                                            gcaAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAg                                <  2:1000853/140‑1 (MQ=255)                                                                                             caAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTc                                                >  2:576888/1‑123 (MQ=255)                                                                                             caAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTc                                                <  1:576888/123‑1 (MQ=255)                                                                                             caAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGc                               >  2:450884/1‑140 (MQ=255)                                                                                             caAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGc                               >  2:32733/1‑140 (MQ=255)                                                                                              aaaCCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCa                              <  2:242366/140‑1 (MQ=255)                                                                                                         cATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCca                      >  1:293644/1‑137 (MQ=255)                                                                                                         cATCAATGCCTGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCca                      <  2:293644/137‑1 (MQ=255)                                                                                                                   tGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGccc         >  2:866884/1‑140 (MQ=255)                                                                                                                   tGCGCGAGCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGccc         <  2:917076/140‑1 (MQ=255)                                                                                                                          gCGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTa  <  1:450884/140‑1 (MQ=255)                                                                                                                           cGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGccc         <  2:1098471/132‑1 (MQ=255)                                                                                                                           cGGTGAGAGCGCTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGccc         >  1:1098471/1‑132 (MQ=255)                                                                                                                                      |                                                                                                                                GCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTA  >  NC_000913/2566046‑2566306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑