Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	275,984	C→T	T220T (ACC→ACT)	mmuP →	S‑methyl‑L‑methionine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	275,984	0	C	T	100.0%	208.7 / NA	59	T220T (ACC→ACT)	mmuP	S‑methyl‑L‑methionine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/35);  total (24/35)

TTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGA  >  NC_000913/275850‑276117                                                                                                                                       |                                                                                                                                      ttCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCt                                                                                                                                  >  1:996563/1‑140 (MQ=255)        ttATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGc                                                                                                                           >  1:717615/1‑140 (MQ=255)        ttATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGc                                                                                                                           >  2:478598/1‑140 (MQ=255)             ccGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTgc                                                                                                                      <  1:478598/140‑1 (MQ=255)                aTGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTgccgc                                                                                                                   <  2:248746/140‑1 (MQ=255)                aTGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTgccgc                                                                                                                   >  1:356042/1‑140 (MQ=255)                    aGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGt                                                                                                               <  2:973/140‑1 (MQ=255)                     ggATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTg                                                                                                              >  1:527727/1‑140 (MQ=255)                           cTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACgg                                                                                                        >  2:152883/1‑140 (MQ=255)                           cTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACgg                                                                                                        >  1:140558/1‑140 (MQ=255)                              gCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGaaa                                                                                                     >  1:119897/1‑140 (MQ=255)                                ccGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAAc                                                                                                   >  2:172890/1‑140 (MQ=255)                                ccGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGC‑TTTTCGGGTACTGAGCTTATCGGCATTGccgccg                                                                                                                 >  2:742080/1‑125 (MQ=255)                                ccGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGC‑TTTTCGGGTACTGAGCTTATCGGCATTGccgccg                                                                                                                 <  1:742080/125‑1 (MQ=255)                                     ccGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCcgcg                                                                                              <  1:1175546/140‑1 (MQ=255)                                     ccGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCcgcg                                                                                              <  2:69836/140‑1 (MQ=255)                                       ggggCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCa                                                                                            <  2:140558/140‑1 (MQ=255)                                         ggCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCaaa                                                                                          <  1:83143/140‑1 (MQ=255)                                           cTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGt                                                                                        <  2:356042/140‑1 (MQ=255)                                           cTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGt                                                                                        <  1:531078/140‑1 (MQ=255)                                              aGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGAGCAAAGTTAt                                                                                     <  1:148420/140‑1 (MQ=255)                                                     tCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTa                                                                              <  1:1324242/140‑1 (MQ=255)                                                        cGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCg                                                                           <  1:582600/140‑1 (MQ=255)                                                        cGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCg                                                                           >  1:937504/1‑140 (MQ=255)                                                         ggCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGa                                                                          <  1:762437/140‑1 (MQ=255)                                                         ggCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGa                                                                          <  1:915654/140‑1 (MQ=255)                                                         ggCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGa                                                                          <  2:119897/140‑1 (MQ=255)                                                                        cccGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATcg                                                           <  2:871770/140‑1 (MQ=255)                                                                          cGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATcgcg                                                         <  2:1177764/140‑1 (MQ=255)                                                                                gTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTg                                                   >  1:1341940/1‑140 (MQ=255)                                                                                    cTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGa                                                  <  1:1068593/137‑1 (MQ=255)                                                                                    cTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGa                                                  >  2:1068593/1‑137 (MQ=255)                                                                                           aTTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCtt                                        <  2:717615/140‑1 (MQ=255)                                                                                             tttGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTa                                      >  1:553417/1‑140 (MQ=255)                                                                                                   gaCTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCa                                <  1:1345729/140‑1 (MQ=255)                                                                                                   gaCTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCa                                <  1:172890/140‑1 (MQ=255)                                                                                                   gaCTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCa                                <  2:478583/140‑1 (MQ=255)                                                                                                   gaCTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCAGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCa                                <  1:193047/140‑1 (MQ=255)                                                                                                    aCTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCAc                               <  1:152883/140‑1 (MQ=255)                                                                                                     cTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCAcc                              <  1:507341/140‑1 (MQ=255)                                                                                                      tATGGTGGCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCg                             >  1:87977/1‑140 (MQ=255)                                                                                                          gtggCAGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGtt                         <  2:527727/140‑1 (MQ=255)                                                                                                               aGTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGc                    >  1:478369/1‑140 (MQ=255)                                                                                                                gTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCt                   >  1:1145462/1‑140 (MQ=255)                                                                                                                gTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCt                   >  2:828696/1‑140 (MQ=255)                                                                                                                gTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCt                   >  2:760988/1‑140 (MQ=255)                                                                                                                gTGAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCt                   >  1:268375/1‑140 (MQ=255)                                                                                                                  gAACTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTgg                 >  1:176250/1‑140 (MQ=255)                                                                                                                    aCTTTGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCa               <  2:1145462/140‑1 (MQ=255)                                                                                                                       ttGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAgcg            >  2:720850/1‑140 (MQ=255)                                                                                                                        tGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAgcgc           <  1:276642/140‑1 (MQ=255)                                                                                                                        tGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAgcgc           >  2:276642/1‑140 (MQ=255)                                                                                                                        tGCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAgcgc           <  2:1217861/140‑1 (MQ=255)                                                                                                                         gCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCt          <  2:478369/140‑1 (MQ=255)                                                                                                                         gCTTTTTCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCt          <  2:906440/140‑1 (MQ=255)                                                                                                                             tttCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATc      >  1:553626/1‑140 (MQ=255)                                                                                                                               tCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATccc    <  2:176250/140‑1 (MQ=255)                                                                                                                               tCGGGTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATccc    <  2:553417/140‑1 (MQ=255)                                                                                                                                 gggTACTGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGa  <  1:209070/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      TTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGA  >  NC_000913/275850‑276117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑