Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,658,666	T→C	G90G (GGA→GGG)	hscA ←	iron‑sulfur cluster biosynthesis chaperone HscA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,658,666	0	T	C	100.0%	252.9 / NA	70	G90G (GGA→GGG)	hscA	iron‑sulfur cluster biosynthesis chaperone HscA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/36);  total (34/36)

AGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGTCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCTTCATGAT  >  NC_000913/2658535‑2658799                                                                                                                                    |                                                                                                                                      aGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAg                                                                                                                               <  1:1329331/140‑1 (MQ=255)    aTGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCg                                                                                                                            <  2:250732/140‑1 (MQ=255)     tGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGt                                                                                                                           >  1:23897/1‑140 (MQ=255)      gTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATc                                                                                                                                 >  1:29309/1‑133 (MQ=255)      gTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATc                                                                                                                                 <  2:29309/133‑1 (MQ=255)      gTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGtt                                                                                                                          <  1:684483/140‑1 (MQ=255)      gTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGtt                                                                                                                          <  1:418793/140‑1 (MQ=255)      gTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGtt                                                                                                                          >  1:347089/1‑140 (MQ=255)       tCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGttt                                                                                                                         >  2:407677/1‑140 (MQ=255)         cGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTaa                                                                                                                       <  2:372403/140‑1 (MQ=255)         cGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTaa                                                                                                                       >  1:255922/1‑140 (MQ=255)          gaaGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAAc                                                                                                                      <  1:242213/138‑1 (MQ=255)          gCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAAc                                                                                                                      <  1:358931/140‑1 (MQ=255)          gCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAAc                                                                                                                      <  2:558859/140‑1 (MQ=255)          gCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAAc                                                                                                                      <  2:255922/140‑1 (MQ=255)           cAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAAc                                                                                                                      <  2:1351602/139‑1 (MQ=255)           cAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAAc                                                                                                                      >  1:1351602/1‑139 (MQ=255)             gAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGa                                                                                                                   <  2:163564/140‑1 (MQ=255)                aCGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACt                                                                                                                >  2:747627/1‑140 (MQ=255)                 cgcgCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTa                                                                                                               >  1:576084/1‑140 (MQ=255)                  gcgcACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTaa                                                                                                              <  2:347089/140‑1 (MQ=255)                   cgcACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAAt                                                                                                             >  1:1211099/1‑140 (MQ=255)                   cgcACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAAt                                                                                                             <  1:747627/140‑1 (MQ=255)                    gcACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAAtt                                                                                                            <  1:665176/140‑1 (MQ=255)                      aCCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATtgt                                                                                                          <  2:650947/140‑1 (MQ=255)                           gTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTgg                                                                                                     >  1:649627/1‑140 (MQ=255)                                 cagcCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTAt                                                                                               >  1:290521/1‑140 (MQ=255)                                     cccGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAg                                                                                           >  2:1288794/1‑140 (MQ=255)                                     cccGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAg                                                                                           >  1:731793/1‑140 (MQ=255)                                     cccGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAg                                                                                           <  2:261663/140‑1 (MQ=255)                                     cccGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAg                                                                                           <  2:290521/140‑1 (MQ=255)                                     cccGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTCACGGTATCGAg                                                                                           <  2:13510/140‑1 (MQ=255)                                       cgccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAgcg                                                                                         <  1:407677/140‑1 (MQ=255)                                       cgccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAgcg                                                                                         <  2:335175/140‑1 (MQ=255)                                       cgccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAgcg                                                                                         <  2:731793/140‑1 (MQ=255)                                                         cGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGt                                                                       >  2:988627/1‑140 (MQ=255)                                                            cAGGCCGTTTTCGCTGCCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCAt                                                                    <  2:1224750/140‑1 (MQ=255)                                                               gCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAAc                                                                 >  1:752298/1‑140 (MQ=255)                                                               gCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAAc                                                                 >  1:1066084/1‑140 (MQ=255)                                                                ccGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAAcc                                                                <  1:1288794/140‑1 (MQ=255)                                                                   ttttCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCAc                                                             >  2:300247/1‑140 (MQ=255)                                                                      tCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGa                                                          <  2:576084/140‑1 (MQ=255)                                                                            gCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGccc                                                    <  1:125649/140‑1 (MQ=255)                                                                                      gATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCt                                               >  2:532825/1‑135 (MQ=255)                                                                                      gATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCt                                               <  1:532825/135‑1 (MQ=255)                                                                                           ggCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTg                                     >  2:502995/1‑140 (MQ=255)                                                                                           ggCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTg                                     >  1:173119/1‑140 (MQ=255)                                                                                            gCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGa                                    <  2:1066084/140‑1 (MQ=255)                                                                                             cAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGaa                                   <  1:988627/140‑1 (MQ=255)                                                                                             cAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGaa                                   <  2:649627/140‑1 (MQ=255)                                                                                             cAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGaa                                   <  2:23897/140‑1 (MQ=255)                                                                                                aTGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGa                                          >  1:771958/1‑130 (MQ=255)                                                                                                aTGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGa                                          <  2:771958/130‑1 (MQ=255)                                                                                                aTGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGaacaa                                >  2:547331/1‑140 (MQ=255)                                                                                                aTGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGaa                                   >  2:317697/1‑137 (MQ=255)                                                                                                aTGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGaa                                   <  1:317697/137‑1 (MQ=255)                                                                                                 tGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGaacaac                               >  2:746036/1‑140 (MQ=255)                                                                                                 tGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGATATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGaacaac                               >  1:428193/1‑140 (MQ=255)                                                                                                          cGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGgcag                      >  1:1313627/1‑140 (MQ=255)                                                                                                            ttGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGgcagca                    >  1:173657/1‑140 (MQ=255)                                                                                                            ttGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGC‑GCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGcagcag                   >  2:1073523/1‑140 (MQ=255)                                                                                                                   atatCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACg             <  2:173657/140‑1 (MQ=255)                                                                                                                       cagccagcGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCt         >  2:421936/1‑140 (MQ=255)                                                                                                                         gccagcGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCTTc       >  2:556961/1‑140 (MQ=255)                                                                                                                         gccagcGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCTTc       >  2:504959/1‑140 (MQ=255)                                                                                                                          ccagcGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACgg            <  2:1311672/134‑1 (MQ=255)                                                                                                                          ccagcGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACgg            >  1:1311672/1‑134 (MQ=255)                                                                                                                            agcgagcgCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGa               <  2:111650/129‑1 (MQ=255)                                                                                                                            agcgagcgCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGa               >  1:111650/1‑129 (MQ=255)                                                                                                                            agcgagcgCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCTTCATg    <  1:421936/140‑1 (MQ=255)                                                                                                                              cgagcgCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCTTCATGAt  >  1:830813/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                      AGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGTCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCTTCATGAT  >  NC_000913/2658535‑2658799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑