Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,790,113	C→G	G44G (GGC→GGG)	lhgO →	L‑2‑hydroxyglutarate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,790,113	0	C	G	100.0%	181.9 / NA	50	G44G (GGC→GGG)	lhgO	L‑2‑hydroxyglutarate oxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (24/26);  total (24/26)

CGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGG  >  NC_000913/2789979‑2790244                                                                                                                                       |                                                                                                                                    cGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCacaa                                                                                                                                >  1:437711/1‑140 (MQ=255) cGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCacaa                                                                                                                                >  1:57661/1‑140 (MQ=255)    gtGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAg                                                                                                                             <  2:109945/139‑1 (MQ=255)    aTGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAg                                                                                                                             >  2:623995/1‑140 (MQ=255)        aTGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAgcggc                                                                                                                         <  2:342495/140‑1 (MQ=255)        aTGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAgcggc                                                                                                                         <  2:310673/140‑1 (MQ=255)        aTGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAgcggc                                                                                                                         <  2:1301865/140‑1 (MQ=255)         tGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAgcggcg                                                                                                                        <  2:827231/140‑1 (MQ=255)         tGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAgcggcg                                                                                                                        >  2:284571/1‑140 (MQ=255)         tGATTTTGTGATTAGTGGCGGCGGCATCATGGGCATGTCGACCGCCATGCAAGTGATTGATGTCTATCCGGACGCCGGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCTTGTCACCAGACGGGGCACAACAgcggcg                                                                                                                        <  2:303549/140‑1 (MQ=255)              ttGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATc                                                                                                                   <  2:813516/140‑1 (MQ=255)              ttGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATc                                                                                                                   >  2:698056/1‑140 (MQ=255)               tgtgATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATcc                                                                                                                  >  1:889670/1‑140 (MQ=255)               tgtgATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATcc                                                                                                                  >  1:811671/1‑140 (MQ=255)                 tgATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCAt                                                                                                                >  1:1279261/1‑140 (MQ=255)                  gATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATg                                                                                                               <  1:170105/140‑1 (MQ=255)                     tattGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCg                                                                                                            <  2:811671/140‑1 (MQ=255)                       ttGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCggg                                                                                                          <  1:739835/140‑1 (MQ=255)                       ttGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCggg                                                                                                          <  2:1080769/140‑1 (MQ=255)                       ttGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCggg                                                                                                          <  1:284571/140‑1 (MQ=255)                              cggcATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTAtt                                                                                                   <  1:623995/140‑1 (MQ=255)                              cggcATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTAtt                                                                                                   >  2:809208/1‑140 (MQ=255)                                 catcatCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTaca                                                                                                >  1:784723/1‑140 (MQ=255)                                    catcGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGc                                                                                             >  1:286434/1‑140 (MQ=255)                                           aTGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAg                                                                                      <  2:286434/140‑1 (MQ=255)                                            tGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGc                                                                                     >  2:802141/1‑140 (MQ=255)                                             gTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTACGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGcc                                                                                    >  1:1193079/1‑140 (MQ=255)                                                 aCCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGaa                                                                                >  1:182409/1‑140 (MQ=255)                                                    gCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGgc                                                                             <  1:802141/140‑1 (MQ=255)                                                     ccATGCACCTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGgcg                                                                            <  2:94282/140‑1 (MQ=255)                                                     ccATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGgcg                                                                            <  2:195966/140‑1 (MQ=255)                                                        tGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAg                                                                         >  2:563551/1‑140 (MQ=255)                                                               gattgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGcc                                                                  <  2:889670/140‑1 (MQ=255)                                                                attgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCt                                                                 >  1:376195/1‑140 (MQ=255)                                                                attgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCt                                                                 >  2:801994/1‑140 (MQ=255)                                                                  tgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTgg                                                               >  2:900606/1‑140 (MQ=255)                                                                                 cGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGccc                                                >  2:1103763/1‑139 (MQ=255)                                                                                   cccGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGccacc                                              <  2:437711/140‑1 (MQ=255)                                                                                   cccGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGccacc                                              <  2:57661/140‑1 (MQ=255)                                                                                            cGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCttt                                     >  1:48550/1‑140 (MQ=255)                                                                                              ttGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTg                                   >  2:967023/1‑140 (MQ=255)                                                                                                  tGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGAt                               <  2:1279863/140‑1 (MQ=255)                                                                                                    gAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCa                             <  1:958938/140‑1 (MQ=255)                                                                                                    gAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCa                             >  1:807862/1‑140 (MQ=255)                                                                                                           agTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGc                      <  2:48550/140‑1 (MQ=255)                                                                                                            gTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCa                     <  1:698056/140‑1 (MQ=255)                                                                                                            gTCCGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCa                     >  2:529215/1‑140 (MQ=255)                                                                                                              ccGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCa                     >  2:324313/1‑138 (MQ=255)                                                                                                              ccGCCCCGGCCTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCa                     <  1:324313/138‑1 (MQ=255)                                                                                                                       ccTGTCACCAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGAc          <  2:182409/140‑1 (MQ=255)                                                                                                                               cAGACGGGGCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCgg  <  1:563551/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    CGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGG  >  NC_000913/2789979‑2790244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑