Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,177,829	(A)7→6	coding (82/630 nt)	nudF ←	ADP‑sugar pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,177,823	0	A	.	100.0%	229.8 / NA	54	coding (88/630 nt)	nudF	ADP‑sugar pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (25/29);  total (25/29)

AGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGAC  >  NC_000913/3177690‑3177958                                                                                                                                      |                                                                                                                                        aGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGGGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaaag                                                                                                                                   <  1:467000/140‑2 (MQ=255)        cTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGAt                                                                                                                           <  1:512863/140‑1 (MQ=255)             tcaCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAgt                                                                                                                      >  1:424126/1‑140 (MQ=255)             tcaCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAgt                                                                                                                      >  1:276059/1‑140 (MQ=255)                  cACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTtc                                                                                                                 <  1:249281/140‑1 (MQ=255)                   ccTGGGTCAAAGGGTAGCAAGCCTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTtct                                                                                                                <  2:1083458/139‑1 (MQ=255)                   aCTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTtct                                                                                                                >  1:883061/1‑140 (MQ=255)                      gggTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGt                                                                                                             <  2:1023288/140‑1 (MQ=255)                      gggTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTtctctg                                                                                                             >  1:287762/1‑138 (MQ=255)                      gggTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGt                                                                                                             >  2:32187/1‑140 (MQ=255)                       ggTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCGTTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTg                                                                                                            >  2:1079055/1‑140 (MQ=255)                        gTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGc                                                                                                           >  2:403433/1‑140 (MQ=255)                         tCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCa                                                                                                          <  2:276059/140‑1 (MQ=255)                           aaggggTAGCAAGCCTGCGGCGTGCCCGCGCTAAAAATTTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                                                                                        <  2:981666/137‑1 (MQ=255)                              gggTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCaataat                                                                                                     <  2:424126/140‑1 (MQ=255)                                  aGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCt                                                                                                 >  1:439349/1‑140 (MQ=255)                                         cTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGt                                                                                          >  1:770178/1‑140 (MQ=255)                                           gcggcgTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttt                                                                                        >  2:698825/1‑140 (MQ=255)                                            cggcgTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGtttt                                                                                       >  1:89129/1‑140 (MQ=255)                                                 gcaCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCa                                                                                  <  2:770178/138‑1 (MQ=255)                                                        gcTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTaa                                                                           <  1:506105/140‑1 (MQ=255)                                                            aaaaaTTTCCCGCCGCACCTCATGACTCGTTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACggg                                                                       <  1:1079055/140‑1 (MQ=255)                                                                            aCCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTa                                                       <  2:77280/140‑1 (MQ=255)                                                                             ccTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTaa                                                      <  2:916568/140‑1 (MQ=255)                                                                              cTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     <  2:883061/140‑1 (MQ=255)                                                                              cTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     <  2:986606/140‑1 (MQ=255)                                                                              cTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     <  1:17886/140‑1 (MQ=255)                                                                              cTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     <  1:245315/140‑1 (MQ=255)                                                                              cTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     <  1:698825/140‑1 (MQ=255)                                                                                cATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCa                                                   <  1:586901/140‑1 (MQ=255)                                                                                      tCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTa                                             >  1:421687/1‑140 (MQ=255)                                                                                        aTTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     <  1:274315/130‑1 (MQ=255)                                                                                        aTTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     >  2:274315/1‑130 (MQ=255)                                                                                        aTTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAAt                                           >  2:419916/1‑140 (MQ=255)                                                                                          ttGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGt                                         >  1:503888/1‑140 (MQ=255)                                                                                           tGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTc                                        <  2:503888/140‑1 (MQ=255)                                                                                           tGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTc                                        <  1:32187/140‑1 (MQ=255)                                                                                           tGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTc                                        <  2:421687/140‑1 (MQ=255)                                                                                             cccGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCt                                      <  1:254787/140‑1 (MQ=255)                                                                                             cccGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCt                                      <  1:728574/140‑1 (MQ=255)                                                                                             cccGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCt                                      >  2:254787/1‑140 (MQ=255)                                                                                                    aaTAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAAt                                           <  1:1042733/128‑1 (MQ=255)                                                                                                    aaTAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAAt                                           >  2:1042733/1‑128 (MQ=255)                                                                                                       aGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATa                            <  2:89129/140‑1 (MQ=255)                                                                                                        gACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAg                           <  2:355618/140‑1 (MQ=255)                                                                                                        gACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAg                           <  2:902310/140‑1 (MQ=255)                                                                                                              gACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCt                                      <  2:18816/123‑1 (MQ=255)                                                                                                              gACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCt                                      >  1:18816/1‑123 (MQ=255)                                                                                                              gACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAAtt                     >  2:643923/1‑140 (MQ=255)                                                                                                                  aaaTCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAAt                 >  2:1046898/1‑140 (MQ=255)                                                                                                                   aaTCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAAtg                >  1:878397/1‑140 (MQ=255)                                                                                                                               tCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCg    >  2:1044007/1‑140 (MQ=255)                                                                                                                               tCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCg    >  1:995560/1‑140 (MQ=255)                                                                                                                                 tAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGAc  >  1:868610/1‑140 (MQ=255)                                                                                                                                 tAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGAc  >  1:1122295/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        AGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGAC  >  NC_000913/3177690‑3177958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑