Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	828,947	(T)5→4	coding (35/1737 nt)	ybhF ←	putative ABC exporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	828,943	0	T	.	100.0%	214.6 / NA	51	coding (39/1737 nt)	ybhF	putative ABC exporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (24/27);  total (24/27)

GTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGT  >  NC_000913/828807‑829077                                                                                                                                         |                                                                                                                                       gTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGtttt                                                                                                                                     >  1:636925/1‑140 (MQ=255)     cAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGttttccag                                                                                                                                 >  2:101600/1‑136 (MQ=255)     cAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGttttccag                                                                                                                                 >  1:408815/1‑136 (MQ=255)             ccGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCa                                                                                                                        >  1:717581/1‑140 (MQ=255)                ccAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCg                                                                                                                     >  1:463205/1‑140 (MQ=255)                 cAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGc                                                                                                                      <  1:1096687/138‑1 (MQ=255)                 cAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGc                                                                                                                      >  2:1096687/1‑138 (MQ=255)                          cATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGc                                                                                                           <  1:1102785/140‑1 (MQ=255)                            tCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGcat                                                                                                         <  2:463205/140‑1 (MQ=255)                               gCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGcatcat                                                                                                      >  2:830938/1‑140 (MQ=255)                               gCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGcatcat                                                                                                      >  1:166946/1‑140 (MQ=255)                                cGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCAtcatt                                                                                                     <  2:703591/140‑1 (MQ=255)                                 gTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCAtcattc                                                                                                    <  2:408815/140‑1 (MQ=255)                                    gTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATg                                                                                                 >  2:180139/1‑140 (MQ=255)                                             gCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTc                                                                                        >  1:54203/1‑140 (MQ=255)                                              cACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCg                                                                                       <  1:115901/140‑1 (MQ=255)                                               aCCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGt                                                                                      >  2:1031448/1‑140 (MQ=255)                                                 cGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCa                                                                                    <  2:166946/140‑1 (MQ=255)                                                   tCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCAcc                                                                                  >  1:1087603/1‑140 (MQ=255)                                                   tCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑GTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCAcc                                                                                  >  2:455471/1‑140 (MQ=255)                                                                aCCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTc                                                                     <  1:289313/140‑1 (MQ=255)                                                                 ccccGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCa                                                                    >  2:930894/1‑140 (MQ=255)                                                                       cacaTAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGTCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCa                                                              <  2:523631/140‑1 (MQ=255)                                                                               cGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTgg                                                      >  1:759282/1‑140 (MQ=255)                                                                                    tGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTaa                                                 >  1:59912/1‑140 (MQ=255)                                                                                    tGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTaa                                                 <  1:1031448/140‑1 (MQ=255)                                                                                    tGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTaa                                                 <  1:443403/140‑1 (MQ=255)                                                                                      aaTGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACg                                               >  2:724680/1‑140 (MQ=255)                                                                                      aaTGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACg                                               <  2:717581/140‑1 (MQ=255)                                                                                        tGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGca                                             >  1:734790/1‑140 (MQ=255)                                                                                                tCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGc                                     >  2:925642/1‑140 (MQ=255)                                                                                                 cGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCg                                    <  2:636925/140‑1 (MQ=255)                                                                                                   aGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTc                                  >  1:519623/1‑140 (MQ=255)                                                                                                   aGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTc                                  <  1:925642/140‑1 (MQ=255)                                                                                                   aGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTc                                  >  1:937610/1‑140 (MQ=255)                                                                                                     cggcgCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGTATTCCCTGGCGTAACGCATCATCGGCg                                    <  2:308965/136‑1 (MQ=255)                                                                                                     cggcgCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGTATTCCCTGGCGTAACGCATCATCGGCg                                    >  1:308965/1‑136 (MQ=255)                                                                                                     cggcgCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCgg                                >  1:627788/1‑140 (MQ=255)                                                                                                     cggcgCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCATCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCgg                                >  2:1083866/1‑140 (MQ=255)                                                                                                      ggcgCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGt                               <  1:125770/140‑1 (MQ=255)                                                                                                      ggcgCGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGt                               <  2:12982/140‑1 (MQ=255)                                                                                                       gcgcGACGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTc                              <  2:59912/140‑1 (MQ=255)                                                                                                            aCGGCGGGCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTcaccac                         >  1:360515/1‑140 (MQ=255)                                                                                                                  ggCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACg                   <  1:724680/140‑1 (MQ=255)                                                                                                                  ggCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACg                   <  2:54203/140‑1 (MQ=255)                                                                                                                  ggCTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACg                   <  2:734790/140‑1 (MQ=255)                                                                                                                    cTTGTCCATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCa                 <  1:930894/140‑1 (MQ=255)                                                                                                                         ccATGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTGACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGa            <  2:836981/140‑1 (MQ=255)                                                                                                                            tGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAg         >  1:542025/1‑140 (MQ=255)                                                                                                                            tGCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAg         >  2:226921/1‑140 (MQ=255)                                                                                                                             gCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACg                   <  1:350114/129‑1 (MQ=255)                                                                                                                             gCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACg                   >  2:350114/1‑129 (MQ=255)                                                                                                                             gCCCGGAAAGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGa        >  2:434255/1‑140 (MQ=255)                                                                                                                                   aaaGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCCCCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGNCGGTCACCACAATACGCAGGCGATAGACGAGGt  <  1:226921/140‑1 (MQ=255)                                                                                                                                   aaaGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGt  <  2:627788/140‑1 (MQ=255)                                                                                                                                   aaaGCG‑TTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGt  <  2:360515/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGT  >  NC_000913/828807‑829077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑