Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	361,790	(T)6→7	coding (71/612 nt)	lacA ←	galactoside O‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	361,784	1	.	T	100.0%	292.8 / NA	82	R26K (AGA→AAA)	lacA	galactoside O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (43/39);  total (43/39)

AGGCGGTTCTACCCAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑C‑‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGA  >  NC_000913/361660‑361915                                                                                                                               |                                                                                                                                     aGGCGGTTCTACCCAGGCGTTTTTCCCTACCCTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTTAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAg                                                                                                                       >  1:1006982/1‑140 (MQ=255)         cTACCCAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGca                                                                                                               <  1:791844/140‑1 (MQ=255)           aCCCAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGcaca                                                                                                             <  1:915279/140‑1 (MQ=255)           aCCCAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGcaca                                                                                                             <  1:877336/140‑1 (MQ=255)           aCCCAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGcaca                                                                                                             <  1:178966/140‑1 (MQ=255)             ccAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACata                                                                                                           <  1:757401/140‑1 (MQ=255)              cAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACatat                                                                                                          >  2:33185/1‑140 (MQ=255)               aGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATc                                                                                                         <  1:543616/140‑1 (MQ=255)                 gCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACata                                                                                                           >  2:421577/1‑136 (MQ=255)                 gCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACata                                                                                                           <  1:421577/136‑1 (MQ=255)                 gCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCgg                                                                                                       >  1:768074/1‑140 (MQ=255)                   gTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACACTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTa                                                                                                     <  1:336352/140‑1 (MQ=255)                      ttCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAAt                                                                                                  >  1:787585/1‑140 (MQ=255)                      ttCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAAt                                                                                                  >  1:287596/1‑140 (MQ=255)                        ccccTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAg                                                                                                <  1:33185/140‑1 (MQ=255)                             aCCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGc                                                                                           <  2:118093/140‑1 (MQ=255)                             aCCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGc                                                                                           >  1:443556/1‑140 (MQ=255)                              ccGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGcc                                                                                          <  1:135394/140‑1 (MQ=255)                              ccGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGcc                                                                                          <  2:654406/140‑1 (MQ=255)                              ccGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGcc                                                                                          >  2:147446/1‑140 (MQ=255)                              ccGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGcc                                                                                          <  2:548694/140‑1 (MQ=255)                               cGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCt                                                                                         >  1:367280/1‑140 (MQ=255)                               cGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCt                                                                                         >  1:669227/1‑140 (MQ=255)                               cGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCt                                                                                         >  2:628623/1‑140 (MQ=255)                                 tGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGc                                                                                       >  1:338502/1‑140 (MQ=255)                                 tGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGc                                                                                       >  2:970054/1‑140 (MQ=255)                                 tGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGc                                                                                       >  1:326084/1‑140 (MQ=255)                                 tGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGc                                                                                       >  2:577450/1‑140 (MQ=255)                                   gCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGctc                                                                                     >  1:757408/1‑140 (MQ=255)                                      aaCATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTa                                                                                  <  2:443556/140‑1 (MQ=255)                                            tCTTTAATGCGGTTTTCTCTTATTTCAACTTCTGATGGATGCGAGTGATTAAAATCATACAATAACGTTTTTCCACGAAGTAT‑‑TTTTTTCCGGTAAGCCTTCGCATATATCGGTAAATAGCTTGCCTGCTCTTATTCttt                                                                            <  2:749510/140‑1 (MQ=255)                                            tCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCttt                                                                            <  2:367280/140‑1 (MQ=255)                                                   tCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCAt                                                                     >  2:592940/1‑140 (MQ=255)                                                    cAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCa                                                                      <  2:613821/138‑1 (MQ=255)                                                    cAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCa                                                                      >  1:613821/1‑138 (MQ=255)                                                       gCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGc                                                                 >  1:1118698/1‑140 (MQ=255)                                                       gCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGc                                                                 >  2:283721/1‑140 (MQ=255)                                                           tctcTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGt                                                             >  2:1047678/1‑140 (MQ=255)                                                            ctctTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGtt                                                            >  2:660135/1‑140 (MQ=255)                                                               ttttttCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCaa                                                         <  1:147446/140‑1 (MQ=255)                                                                 ttttCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATg                                                              <  1:1058188/133‑1 (MQ=255)                                                                 ttttCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATg                                                              >  2:1058188/1‑133 (MQ=255)                                                                     cAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGAt                                                   <  2:287596/140‑1 (MQ=255)                                                                     cAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGAt                                                   <  2:669227/140‑1 (MQ=255)                                                                       aCTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCa                                                 <  2:338502/140‑1 (MQ=255)                                                                       aCTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCa                                                 <  1:970054/140‑1 (MQ=255)                                                                         ttCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACt                                               <  2:787585/140‑1 (MQ=255)                                                                         ttCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACt                                               <  2:326084/140‑1 (MQ=255)                                                                         ttCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACt                                               <  2:730992/140‑1 (MQ=255)                                                                            tGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCg                                            <  1:731057/140‑1 (MQ=255)                                                                                   tgCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACt                                               <  1:933428/130‑1 (MQ=255)                                                                                   tgCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACt                                               >  2:933428/1‑130 (MQ=255)                                                                                   tgCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGat                                     >  1:992417/1‑140 (MQ=255)                                                                                     cGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGatat                                   >  2:711107/1‑140 (MQ=255)                                                                                     cGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATAGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGatat                                   >  2:477635/1‑140 (MQ=255)                                                                                      gAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATg                                  >  2:1044839/1‑140 (MQ=255)                                                                                       aGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGt                                 >  2:1031844/1‑140 (MQ=255)                                                                                        gTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGtt                                >  2:1086161/1‑140 (MQ=255)                                                                                           aTTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGt                             <  1:711107/140‑1 (MQ=255)                                                                                           aTTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGt                             <  1:1031844/140‑1 (MQ=255)                                                                                           aTTAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGt                             >  1:909662/1‑140 (MQ=255)                                                                                            ttAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTc                            <  2:992417/140‑1 (MQ=255)                                                                                             tAAACTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCg                           >  2:449694/1‑140 (MQ=255)                                                                                              aaaCTCATACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCgg                          >  1:806500/1‑140 (MQ=255)                                                                                                    aTACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAgg                    >  1:1031739/1‑140 (MQ=255)                                                                                                     tACATTAACGTTTTCCCACGAAGT‑CTTTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGt                                 >  2:429624/1‑127 (MQ=255)                                                                                                     tACATTAACGTTTTCCCACGAAGT‑CTTTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGt                                 <  1:429624/127‑1 (MQ=255)                                                                                                     tACATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGgc                   >  1:1048267/1‑140 (MQ=255)                                                                                                      aCATTAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGgcg                  >  2:446565/1‑140 (MQ=255)                                                                                                         ttAACGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCCCTCCGTTATGATATGTTGGTCGGATAAGGCGCTc               <  2:1006982/140‑1 (MQ=255)                                                                                                           aaCGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAgg                    >  2:156974/1‑133 (MQ=255)                                                                                                           aaCGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAgg                    <  1:156974/133‑1 (MQ=255)                                                                                                           aaCGTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTcgc             >  1:1111141/1‑140 (MQ=255)                                                                                                            aCGTTTTCCCACCAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTcgcg            >  2:1013891/1‑140 (MQ=255)                                                                                                              gTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGcgcc          <  2:19539/140‑1 (MQ=255)                                                                                                              gTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGcgcc          <  1:678486/140‑1 (MQ=255)                                                                                                              gTTTTCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGcgcc          >  1:19539/1‑140 (MQ=255)                                                                                                                 ttCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCTGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCa       <  2:1031739/140‑1 (MQ=255)                                                                                                                 ttCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCa       <  1:446565/140‑1 (MQ=255)                                                                                                                  tCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCAt      >  2:1113202/1‑140 (MQ=255)                                                                                                                  tCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCAt      >  2:348143/1‑140 (MQ=255)                                                                                                                  tCCCACGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCAt      <  2:909662/140‑1 (MQ=255)                                                                                                                      aCGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGa  <  1:348143/140‑1 (MQ=255)                                                                                                                      aCGAAGT‑CT‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTAATTGGCATGTTCAATGCGATCACTCCGTTGTGATATGTTGGTCGGATAAGTCGCTCGCTCCGCATCCGa  <  2:757408/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                     AGGCGGTTCTACCCAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGT‑C‑‑TTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGA  >  NC_000913/361660‑361915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑