Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,094	(G)8→9	noncoding (72/87 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,086	1	.	G	98.0%	181.9 / ‑3.2	50	noncoding (80/87 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (21/28);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

CAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTT  >  NC_000913/4605950‑4606219                                                                                                                                          |                                                                                                                                       caAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑ggg                                                                                                                                    >  1:785890/1‑140 (MQ=255) caAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑ggg                                                                                                                                    >  1:921967/1‑140 (MQ=255)    aCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCCGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑gggggg                                                                                                                                 <  2:785890/140‑1 (MQ=255)      aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑gggggggg                                                                                                                               <  1:606730/140‑1 (MQ=255)      aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑gggggggg                                                                                                                               >  1:1054503/1‑140 (MQ=255)      aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑gggggggg                                                                                                                               >  1:241499/1‑140 (MQ=255)      aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑gggggggg                                                                                                                               >  1:355176/1‑140 (MQ=255)        ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑ggggggggga                                                                                                                             >  2:48965/1‑138 (MQ=255)        ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGACAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑ggggggggga                                                                                                                             >  2:390474/1‑138 (MQ=255)                  aCATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAAcccc                                                                                                                    <  1:11737/140‑1 (MQ=255)                  aCATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAAcccc                                                                                                                    >  1:941481/1‑140 (MQ=255)                    aTTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCa                                                                                                                  <  1:792898/140‑1 (MQ=255)                     ttttGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACg                                                                                                                <  2:459758/140‑1 (MQ=255)                        tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGaa                                                                                                                        >  1:1077393/1‑130 (MQ=255)                        tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGaa                                                                                                                        <  2:1077393/130‑1 (MQ=255)                        tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTc                                                                                                              <  2:921967/140‑1 (MQ=255)                        tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTc                                                                                                              >  1:636339/1‑140 (MQ=255)                          cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCg                                                                                                            >  1:149014/1‑140 (MQ=255)                            aCCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTa                                                                                                          <  2:149014/140‑1 (MQ=255)                            aCCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGACAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTa                                                                                                          <  1:390474/140‑1 (MQ=255)                             ccATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTa                                                                                                          >  2:467767/1‑140 (MQ=255)                              cATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                        <  2:868229/140‑1 (MQ=255)                                  gTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGaca                                                                                                    <  2:531408/140‑1 (MQ=255)                                     ccTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCa                                                                                                                  >  2:486279/1‑123 (MQ=255)                                     ccTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCa                                                                                                                  <  1:486279/123‑1 (MQ=255)                                     ccTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                                                                                                 <  1:155128/140‑1 (MQ=255)                                       tGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAac                                                                                               >  2:380870/1‑140 (MQ=255)                                          cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                            <  1:380870/140‑1 (MQ=255)                                                       catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAGCACCTGAAGCTAgcg                                                                                 <  2:151765/138‑1 (MQ=255)                                                       catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                                 >  1:151765/1‑138 (MQ=255)                                                       catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                               >  2:113700/1‑140 (MQ=255)                                                       catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                               <  1:48965/140‑1 (MQ=255)                                                       catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                               >  1:168174/1‑140 (MQ=255)                                                       catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                               <  2:553475/140‑1 (MQ=255)                                                          catGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                                            >  1:517350/1‑140 (MQ=255)                                                           atGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                           >  1:766184/1‑140 (MQ=255)                                                                     tAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                 <  2:636339/140‑1 (MQ=255)                                                                      aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACATTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                >  1:369259/1‑140 (MQ=255)                                                                        tGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                        <  2:440882/130‑1 (MQ=255)                                                                        tGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                        >  1:440882/1‑130 (MQ=255)                                                                                        aTGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCt                                              <  2:517350/140‑1 (MQ=255)                                                                                          gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACATTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTa                                            <  2:369259/140‑1 (MQ=255)                                                                                          gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                                              >  1:976690/1‑122 (MQ=255)                                                                                          gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                                              <  2:976690/122‑1 (MQ=255)                                                                                                tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCt                                              <  2:70745/132‑1 (MQ=255)                                                                                                tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCt                                              >  1:70745/1‑132 (MQ=255)                                                                                                 cAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGTCACCTTCGCACAGTCATCTTACtttttt                                     <  2:941481/140‑1 (MQ=255)                                                                                                  aaGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACttttttt                                    >  2:1123357/1‑140 (MQ=255)                                                                                                   aGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAg                                                    <  2:754821/123‑1 (MQ=255)                                                                                                   aGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAg                                                    >  1:754821/1‑123 (MQ=255)                                                                                                          aaCAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                            <  2:355176/140‑1 (MQ=255)                                                                                                          aaCAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGAACTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                            <  2:1054503/140‑1 (MQ=255)                                                                                                                   ggAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGat                                 >  2:131697/1‑126 (MQ=255)                                                                                                                   ggAAGTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGat                                 <  1:131697/126‑1 (MQ=255)                                                                                                                       gTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCg               <  1:156806/140‑1 (MQ=255)                                                                                                                       gTAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCg               >  1:1065615/1‑140 (MQ=255)                                                                                                                        tAAAGATAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTCCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCATCGTTTGGTGCGa              >  2:909286/1‑140 (MQ=255)                                                                                                                             aTAATTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg         <  1:1123357/140‑1 (MQ=255)                                                                                                                               aaTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggggg       <  2:168174/140‑1 (MQ=255)                                                                                                                                    gTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACtt  <  2:241499/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTT  >  NC_000913/4605950‑4606219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑