Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,168,469	(G)5→6	intergenic (‑163/‑243)	yegR ← / → yegS	uncharacterized protein YegR/lipid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,168,464	1	.	G	100.0%	233.3 / NA	62	intergenic (‑158/‑248)	yegR/yegS	uncharacterized protein YegR/lipid kinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (39/23);  total (39/23)

ATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCAT‑GGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTG  >  NC_000913/2168325‑2168597                                                                                                                                             |                                                                                                                                      aTACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCAt                                                                                                                                        >  2:498823/1‑140 (MQ=255) aTACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCAt                                                                                                                                        >  2:333759/1‑140 (MQ=255)   aCCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCAT‑gg                                                                                                                                     <  1:417833/140‑1 (MQ=255)     cAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCAT‑gggg                                                                                                                                   <  1:413650/140‑1 (MQ=255)       aCGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCAT‑gggggg                                                                                                                                 >  2:888304/1‑139 (MQ=255)       aCGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCAT‑gggggg                                                                                                                                 <  1:888304/140‑2 (MQ=255)                 ctAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAatata                                                                                                                        <  1:48188/140‑1 (MQ=255)                 ctAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAatata                                                                                                                        <  2:149425/140‑1 (MQ=255)                   aGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAAtatatt                                                                                                                      >  2:942400/1‑140 (MQ=255)                   aGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCAT‑gggg                                                                                                                                   <  1:559395/126‑1 (MQ=255)                   aGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCAT‑gggg                                                                                                                                   >  2:559395/1‑126 (MQ=255)                        ttAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTa                                                                                                                             >  1:3216/1‑128 (MQ=255)                        ttAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTa                                                                                                                             <  2:3216/128‑1 (MQ=255)                         tAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTg                                                                                                                >  1:695612/1‑140 (MQ=255)                          aaaTATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGGCATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGa                                                                                                               >  2:439339/1‑140 (MQ=255)                          aaaTATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGa                                                                                                               <  1:733950/140‑1 (MQ=255)                          aaaTATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGa                                                                                                               >  1:1087433/1‑140 (MQ=255)                                aataatGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGc                                                                                                         <  1:373303/140‑1 (MQ=255)                                     tGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTg                                                                                                    >  2:1066772/1‑140 (MQ=255)                                       gCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGtt                                                                                                  <  2:133529/140‑1 (MQ=255)                                          tGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACAGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCa                                                                                                       >  1:515279/1‑132 (MQ=255)                                          tGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACAGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCa                                                                                                       <  2:515279/132‑1 (MQ=255)                                            attattATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGtt                                                                                                  <  1:677715/135‑1 (MQ=255)                                            attattATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGtt                                                                                                  >  2:677715/1‑135 (MQ=255)                                             ttattaTAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTg                                                                                            <  2:1076120/140‑1 (MQ=255)                                                      ttGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGtt                                                                                             >  2:344362/1‑130 (MQ=255)                                                      ttGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGtt                                                                                             <  1:344362/130‑1 (MQ=255)                                                      ttGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAgg                                                                                   >  2:434039/1‑140 (MQ=255)                                                      ttGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAgg                                                                                   >  1:812906/1‑140 (MQ=255)                                                      ttGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAgg                                                                                   >  1:236038/1‑140 (MQ=255)                                                           tAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTa                                                                                     <  2:489460/133‑1 (MQ=255)                                                           tAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTa                                                                                     >  1:489460/1‑133 (MQ=255)                                                                        aaTGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGaa                                                                 >  2:98411/1‑140 (MQ=255)                                                                             tCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTa                                                            >  1:730747/1‑140 (MQ=255)                                                                                 tACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCgg                                                        >  1:773303/1‑140 (MQ=255)                                                                                 tACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCgg                                                        >  1:215362/1‑140 (MQ=255)                                                                                    aacaTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTgg                                                     <  2:236038/140‑1 (MQ=255)                                                                                     acaTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGc                                                    <  1:98411/140‑1 (MQ=255)                                                                                      caTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCa                                                   >  1:225616/1‑140 (MQ=255)                                                                                      caTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCa                                                   <  2:812906/140‑1 (MQ=255)                                                                                         ttCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGcc                                                >  2:1029456/1‑140 (MQ=255)                                                                                         ttCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGcc                                                >  2:619563/1‑140 (MQ=255)                                                                                           ccTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGt                                              >  1:457500/1‑140 (MQ=255)                                                                                           ccTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGt                                              >  2:507502/1‑140 (MQ=255)                                                                                             tACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTa                                            >  1:871084/1‑140 (MQ=255)                                                                                                   aGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTc                                      >  1:39173/1‑140 (MQ=255)                                                                                                    gTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCg                                               <  2:749010/130‑1 (MQ=255)                                                                                                    gTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCg                                               >  1:749010/1‑130 (MQ=255)                                                                                                    gTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCg                                     >  2:865490/1‑140 (MQ=255)                                                                                                    gTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCg                                     >  1:522574/1‑140 (MQ=255)                                                                                                         aaTTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTc                                <  1:333759/140‑1 (MQ=255)                                                                                                         aaTTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTc                                <  1:498823/140‑1 (MQ=255)                                                                                                             aCGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCa                            >  1:82318/1‑140 (MQ=255)                                                                                                             aCGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCa                            >  2:548347/1‑140 (MQ=255)                                                                                                              cGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCAt                           >  2:688159/1‑140 (MQ=255)                                                                                                              cGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCAt                           >  2:1088089/1‑140 (MQ=255)                                                                                                               ggACATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCAtt                          >  1:1071689/1‑140 (MQ=255)                                                                                                                  cATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCa                       <  2:215362/140‑1 (MQ=255)                                                                                                                  cATTGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCa                       <  1:1029456/140‑1 (MQ=255)                                                                                                                     tGAGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGc                    >  2:120266/1‑140 (MQ=255)                                                                                                                       aGGCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTc                  <  1:1088089/140‑1 (MQ=255)                                                                                                                        ggCCCTTCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTcc                 >  1:531968/1‑140 (MQ=255)                                                                                                                             ttCAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCtggtg            >  1:578342/1‑140 (MQ=255)                                                                                                                               cAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTc                  <  1:79230/132‑1 (MQ=255)                                                                                                                               cAGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTc                  >  2:79230/1‑132 (MQ=255)                                                                                                                                aGGGTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTa         <  1:193454/140‑1 (MQ=255)                                                                                                                                 gggTAACTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTaa        <  2:730747/140‑1 (MQ=255)                                                                                                                                      aCTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACt   >  2:58672/1‑140 (MQ=255)                                                                                                                                       cTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTg  >  2:375220/1‑140 (MQ=255)                                                                                                                                       cTCCATGGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTg  >  2:768320/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                      ATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCAT‑GGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTG  >  NC_000913/2168325‑2168597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑