| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,326,450 | 0 | T | G | 26.1% | 95.1 / 20.0 | 46 | G114G (GGT→GGG) | atoB | acetyl‑CoA acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/33); new base G (12/0); total (13/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.34e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATA > NC_000913/2326315‑2326580 | aCTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATg‑g < 1:1010937/140‑1 (MQ=255) tAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAat < 2:656129/140‑1 (MQ=255) ggggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGtt < 1:851523/139‑1 (MQ=255) cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGtt < 1:1103553/140‑1 (MQ=255) cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTT‑GAAAATATggggt > 1:1104476/1‑136 (MQ=255) cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTG‑GAAAAAATGAGtt > 2:1103553/1‑140 (MQ=255) cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GGAAAAATGAGtt > 2:1094570/1‑140 (MQ=255) cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GAAAATATGAGtt > 1:983994/1‑140 (MQ=255) ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTa < 1:1014417/140‑1 (MQ=255) tGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGcc < 2:983994/140‑1 (MQ=255) tGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGcc < 1:361305/140‑1 (MQ=255) gggCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGcc < 1:1047955/139‑1 (MQ=255) cAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGccc > 2:38926/1‑138 (MQ=255) cAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGccc < 1:38926/138‑1 (MQ=255) aCGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GAAAAAATTAGTTTAGCCCCCcactt > 2:947239/1‑140 (MQ=255) gtgtGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCtacttact < 2:702523/140‑1 (MQ=255) tgtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GGAAATATGCGGTTAACCCCCTCATTACTc > 2:660776/1‑140 (MQ=255) tgtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GAAAATATGAGTTTAGCCCCCTACTTACTc > 1:164950/1‑140 (MQ=255) tgtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GAAAAAATTAGGTTTGCCCCCCACTTtctc > 2:1047955/1‑140 (MQ=255) tgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGa < 1:89492/140‑1 (MQ=255) aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGc < 1:472490/140‑1 (MQ=255) gTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACg < 1:237835/140‑1 (MQ=255) agacagaaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACg < 1:219864/134‑1 (MQ=255) tCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGc < 1:354007/140‑1 (MQ=255) aaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctc < 2:546212/140‑1 (MQ=255) aaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctc > 2:940190/1‑140 (MQ=255) aTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGTAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctct < 2:791089/140‑1 (MQ=255) aaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTgg < 1:358457/140‑1 (MQ=255) aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GGAAATATGAGTTTTGCCCCCCACTTACTCGATGCAAAAGCAAGCTCTGGTTAtt > 2:354007/1‑139 (MQ=255) aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GGAAAAATGAGTTTAGCCCCCCACTTACTCGATGCAAAAACACGCTCTgggtttc > 2:383446/1‑135 (MQ=255) gtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GGAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGt > 1:1077450/1‑140 (MQ=255) gtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGt > 1:416921/1‑140 (MQ=255) ttCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GGAAAAATGAGTTTTGCCCCCCACTTACTCGATGCAAAAGAACGCCCTGGGTTTCGTCTTg > 2:167297/1‑140 (MQ=255) cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GAAAAAATGAGTTTAGCCCCCCACTTACTCGATGCAAAAACACGCTCTGGTTATCGTCtttgg > 2:16924/1‑137 (MQ=255) gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgag < 1:539546/140‑1 (MQ=255) gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgag < 2:416921/140‑1 (MQ=255) gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgag < 1:153743/140‑1 (MQ=255) gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTG‑GGAAAAATTAGTTTTGCCCCCCCCTTTCTCGATGCAAAAACACGCCCTGGTTATCGTCTTgggg > 2:539546/1‑138 (MQ=255) gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTG‑GAAAAAATGAGTTTAGCCCCCTACTTACTCGATGCAAAAACACGCTCTGGTTATCGGCTTgggg > 1:391531/1‑138 (MQ=255) ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGa < 1:940190/140‑1 (MQ=255) tAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc < 1:16924/140‑1 (MQ=255) tAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGCTTG‑GGAAATATCAGGTTATCCCCCTCCTTACTCTACGGCAAAGCACGCTCTGGCTATTGTCTTGGAGGCCGAc > 2:131893/1‑140 (MQ=255) tgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGAGAGAACAATGGCTTTGCCCCCCACCCACTCAACGGAAAAGCAACCCCTGGTTGTACTCTTGGTGAGGGACAggagtt > 2:1114682/1‑136 (MQ=255) ccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATc < 2:486970/140‑1 (MQ=255) gccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCt < 1:167297/140‑1 (MQ=255) cAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTgcg < 1:131893/140‑1 (MQ=255) cAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTgcg < 1:239392/140‑1 (MQ=255) ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGcgcg < 1:1094570/140‑1 (MQ=255) ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGcgcg < 1:602868/140‑1 (MQ=255) tCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATG‑GAAAATATGAGGTTAGGCCCCCCCCTACTCGAATCAAAATCACGCTCTGGGTTTCGTCTTCGAGACGGACAAGGTTATTACGGAATCCCTCGCGATGGcc > 2:762820/1‑140 (MQ=255) tCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTgcg < 1:365619/140‑1 (MQ=255) cAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCa < 1:91015/140‑1 (MQ=255) gagCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATg < 2:932501/140‑1 (MQ=255) gagCATTGTGGCGGGGGGGGTG‑GGAAAAATGAGGTTAGCCCCCCACTTACTCGATGCAAAAAAACGCTCTGGTTATCGTCTTTGGGACGGGCAGGGTTTTGACGTAAACCTGCGCGGTGGGCTGATGTGCGCCCCCCCTg > 2:1120873/1‑140 (MQ=255) gagCATTGTGGCGGGGGGGATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGGTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATg > 1:789557/1‑140 (MQ=255) gagCATTGTGGCGGGGGGGATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAACACGCTCTGGTTATCGTCTTGGAGACGGACAGGGTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCCCCCCTg > 1:304070/1‑140 (MQ=255) gagCATTGTGGCGGGGGGGATG‑GAAAATATGAGTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCTCTGGGTATCGTCTTGGAGACGGACAGGTTTATGAAGTAAACCTGCGCGGTGGGCTGATGTGCGCCACCCCTg > 2:91015/1‑140 (MQ=255) gagCATTGTGGCGGGGGGGATG‑GAAAAAATGAGGTTTGACCCCTACTTACTCGGTGCAAAAACAACCTCTGGTTATCGTCTTGGAGGCGGAGAGGTTTATTAAGTAATCCTGCGCGATGGCCTGATGTGAGCCACCCATg > 2:494640/1‑140 (MQ=255) agCATTGTGGCGGGGGGGGTG‑GGAAAAATGAGTTTAGCCCCCCACTTACTCG‑‑‑‑‑‑‑AGCACGCTCTCGGTATCGTCTTGGAGGCGGACAGGGTTTTGACGGAATCCTGCGCGGTGGCCTGATGTGCGCCACCCCTGGGTATCat > 2:841522/1‑140 (MQ=255) ggCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCata < 2:61100/140‑1 (MQ=255) | ACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATG‑GAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATA > NC_000913/2326315‑2326580 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |