BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,364,975	1	.	A	46.5%	392.6 / inf	217	coding (2169/2382 nt)	yhcD	putative fimbrial usher protein YhcD
Reads supporting (aligned to +/- strand): ref base . (53/63); new base A (49/52); total (102/115)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 231 total aligned reads displayed.

ATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGATATAAAAGATGAGCAGCAATCATG  >  NC_000913/3364836‑3365112

 tACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑a                                                                                                                                          >  2:474484/1‑140 (MQ=255)

    cGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑aaaa                                                                                                                                       >  1:496326/1‑140 (MQ=255)

      ccAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑aaaaaa                                                                                                                                     >  1:964222/1‑140 (MQ=255)

        aGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACt                                                                                                                                   <  2:1101226/140‑1 (MQ=255)

         gCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTg                                                                                                                                  >  1:831272/1‑140 (MQ=255)

          cTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑aaaaaaactg                                                                                                                                 >  1:706284/1‑136 (MQ=255)

           ttGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGcc                                                                                                                                <  1:307971/140‑1 (MQ=255)

            tGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGcc                                                                                                                                <  1:900986/140‑1 (MQ=255)

              ccAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCa                                                                                                                              >  2:438889/1‑140 (MQ=255)

                 aaaaaTGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAgg                                                                                                                          <  2:23349/140‑1 (MQ=255)

                     aTGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGt                                                                                                                       <  2:783914/140‑1 (MQ=255)

                     aTGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGG‑AAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTC‑ATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTg                                                                                                                     <  2:383104/140‑1 (MQ=255)

                      tGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑CAAAAACTGCCCAGAGGCGTTg                                                                                                                     <  2:806339/140‑1 (MQ=255)

                      tGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTg                                                                                                                     <  1:241246/140‑1 (MQ=255)

                        tCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTTCTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTg                                                                                                                     >  2:965696/1‑139 (MQ=255)

                           atatCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGAtt                                                                                                                <  2:627802/140‑1 (MQ=255)

                           atatCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGAtt                                                                                                                <  2:831272/140‑1 (MQ=255)

                              tCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGa                                                                                                              <  2:174301/140‑1 (MQ=255)

                              tCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGa                                                                                                              <  1:557563/140‑1 (MQ=255)

                                ggTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGAt                                                                                                                 <  2:640856/135‑1 (MQ=255)

                                 gTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGAt                                                                                                          >  2:1039387/1‑140 (MQ=255)

                                    aCGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAg                                                                                                        >  1:48194/1‑140 (MQ=255)

                                       gCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCGCACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGa                                                                                                     >  1:851231/1‑140 (MQ=255)

                                        cacaAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCGCACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGaa                                                                                                    <  2:851231/140‑1 (MQ=255)

                                        cacaAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGaa                                                                                                    <  1:723495/140‑1 (MQ=255)

                                        cacaAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGaa                                                                                                    >  1:130366/1‑140 (MQ=255)

                                          caAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAgg                                                                                                  >  1:662959/1‑140 (MQ=255)

                                          caAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAgg                                                                                                  >  2:926539/1‑140 (MQ=255)

                                          caAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGc                                                                                                 <  1:317827/140‑1 (MQ=255)

                                           aaaaaTGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGc                                                                                                 >  2:7320/1‑140 (MQ=255)

                                           aaaaaTGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGc                                                                                                 <  1:635558/140‑1 (MQ=255)

                                                     aaaCAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGac                                                                                      <  2:876704/140‑1 (MQ=255)

                                                      aaCAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGac                                                                                      <  1:334044/140‑1 (MQ=255)

                                                       aCAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGacaa                                                                                    <  2:956067/140‑1 (MQ=255)

                                                          aGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGacaaca                                                                                  <  1:157093/140‑1 (MQ=255)

                                                          aGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACAt                                                                                 <  2:207369/140‑1 (MQ=255)

                                                          aGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACAt                                                                                 <  2:386661/140‑1 (MQ=255)

                                                              cGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCa                                                                               <  1:1069180/139‑1 (MQ=255)

                                                                tggtgCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACAt                                                                                 >  1:5846/1‑134 (MQ=255)

                                                                 ggtgCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTgg                                                                           >  2:28423/1‑140 (MQ=255)

                                                                 ggtgCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGa                                                                          >  2:658795/1‑140 (MQ=255)

                                                                  gtgCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGAt                                                                         >  2:911796/1‑140 (MQ=255)

                                                                  gtgCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATATGACAACATCAGTGGAt                                                                         >  2:1137929/1‑140 (MQ=255)

                                                                     cAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGa                                                                       <  2:496326/140‑1 (MQ=255)

                                                                      aGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACg                                                                     <  1:444637/140‑1 (MQ=255)

                                                                        tAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGATGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGa                                                                       <  2:964058/136‑1 (MQ=255)

                                                                             aaaGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGtgttgt                                                              >  2:682913/1‑140 (MQ=255)

                                                                               aGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATGTCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTa                                                             >  1:20358/1‑140 (MQ=255)

                                                                                 tCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTAtt                                                           >  1:689413/1‑140 (MQ=255)

                                                                                    gATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCt                                                        >  1:753167/1‑140 (MQ=255)

                                                                                    gATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGgtg                                                                  <  2:525252/129‑1 (MQ=255)

                                                                                    gATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGgtg                                                                  >  1:525252/1‑129 (MQ=255)

                                                                                     aTTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACAGCAGTGGATGACg                                                                     >  1:795516/1‑126 (MQ=255)

                                                                                           tGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACa                                                >  2:598383/1‑140 (MQ=255)

                                                                                            gCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACa                                                <  2:48194/140‑1 (MQ=255)

                                                                                             cGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATc                                              <  1:378401/140‑1 (MQ=255)

                                                                                                  acacAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAAcc                                         <  1:189365/140‑1 (MQ=255)

                                                                                                   cacaACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCg                                        >  2:421295/1‑140 (MQ=255)

                                                                                                     caACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGa                                      <  1:422813/140‑1 (MQ=255)

                                                                                                     caACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGa                                      >  2:337476/1‑140 (MQ=255)

                                                                                                       aCGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGAc                                     <  1:926539/140‑1 (MQ=255)

                                                                                                       aCGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACa                                    <  1:283822/140‑1 (MQ=255)

                                                                                                       aCGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACa                                    <  2:452040/140‑1 (MQ=255)

                                                                                                       aCGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACa                                    <  2:400420/140‑1 (MQ=255)

                                                                                                       aCGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACa                                    <  1:540194/140‑1 (MQ=255)

                                                                                                       aCGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACa                                    <  1:825777/140‑1 (MQ=255)

                                                                                                        cGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACa                                    <  1:969175/140‑1 (MQ=255)

                                                                                                        cGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACa                                    >  2:1050084/1‑140 (MQ=255)

                                                                                                        cGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACa                                    >  1:1045887/1‑140 (MQ=255)

                                                                                                        cGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACa                                    <  1:741550/140‑1 (MQ=255)

                                                                                                        cGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACAt                                   >  1:651185/1‑140 (MQ=255)

                                                                                                           cGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATgg                                 >  1:999119/1‑140 (MQ=255)

                                                                                                            gTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTg                               <  1:1137929/140‑1 (MQ=255)

                                                                                                            gTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTg                               <  1:598383/140‑1 (MQ=255)

                                                                                                            gTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTg                               <  1:682913/140‑1 (MQ=255)

                                                                                                             tGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTg                               >  2:929491/1‑140 (MQ=255)

                                                                                                             tGCATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTg                               >  1:111758/1‑140 (MQ=255)

                                                                                                             tGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAATCCGGACATGGTGc                              >  1:391456/1‑140 (MQ=255)

                                                                                                             tGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGc                              >  2:615881/1‑140 (MQ=255)

                                                                                                             tGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGc                              <  2:328334/140‑1 (MQ=255)

                                                                                                               cATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCt                             <  2:130366/140‑1 (MQ=255)

                                                                                                               cATTACTCAATATCACACTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCt                             <  1:227351/140‑1 (MQ=255)

                                                                                                               cATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTa                            <  2:1052177/140‑1 (MQ=255)

                                                                                                                     tCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGa                          <  1:457977/136‑1 (MQ=255)

                                                                                                                       aaTATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGATATaaa                    >  1:162660/1‑140 (MQ=255)

                                                                                                                         tatCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACa                                    <  1:398174/122‑1 (MQ=255)

                                                                                                                         tatCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCt                             <  2:462160/129‑1 (MQ=255)

                                                                                                                           tCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGATATAAAAGAt                <  1:911796/140‑1 (MQ=255)

                                                                                                                            cacaCTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTa                            <  2:547911/128‑1 (MQ=255)

                                                                                                                            cacaCTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTa                            >  1:547911/1‑128 (MQ=255)

                                                                                                                            cacaCTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGATATAAAAGATg               >  1:950110/1‑140 (MQ=255)

                                                                                                                            cacaCTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGATATAAAAGATg               >  2:729066/1‑140 (MQ=255)

                                                                                                                              cacTTTCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGATATAAAAGATGa              >  1:1029793/1‑140 (MQ=255)

                                                                                                                              cacTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTg                               <  2:722613/122‑1 (MQ=255)

                                                                                                                                 tttCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGATATAAAAGATGagcag          >  2:795444/1‑140 (MQ=255)

                                                                                                                                   tCCGACGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGATATAAAAGATGagcagc         >  2:1122717/1‑140 (MQ=255)

                                                                                                                                   tCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGATATAAAAGATGagc            <  2:745022/136‑1 (MQ=255)

                                                                                                                                   tCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGATATAAAAGATGAgcagca        >  1:469587/1‑140 (MQ=255)

                                                                                                                                       aCGGCAAAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGATATAAAAGATGAGCAGCAATc     <  2:689413/140‑1 (MQ=255)

                                                                                                                                         ggC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGATATAAAAGATGAGCAGCAATCATg  <  1:100693/140‑1 (MQ=255)

ATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGC‑AAAAAACTGCCCAGAGGCGTTGCGATTGAAGATAGTGAAGGCAACTATCTGACAACATCAGTGGATGACGGTGTTGTATTCCTCAATAACATCAAACCGGACATGGTGCTAGATATAAAAGATGAGCAGCAATCATG  >  NC_000913/3364836‑3365112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`