Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,273	A→G	T295A (ACG→GCG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,273	0	A	G	96.9%	212.4 / ‑2.6	65	T295A (ACG→GCG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (37/26);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCC  >  NC_000913/23141‑23409                                                                                                                                     |                                                                                                                                         cgcgCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTaaa                                                                                                                                   >  2:445107/1‑140 (MQ=255) cgcgCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTaaa                                                                                                                                   <  1:241857/140‑1 (MQ=255) cgcgCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTaaa                                                                                                                                   <  1:33518/140‑1 (MQ=255)        tctcTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCg                                                                                                                            >  2:1031175/1‑140 (MQ=255)                gCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGa                                                                                                                    >  2:717586/1‑140 (MQ=255)                 cACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAg                                                                                                                   <  2:576660/140‑1 (MQ=255)                  aCCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAgc                                                                                                                  >  1:457786/1‑140 (MQ=255)                   ccAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAg                                                                                                                   >  1:754223/1‑138 (MQ=255)                   ccAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAg                                                                                                                   <  2:754223/138‑1 (MQ=255)                    cAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAgctg                                                                                                                >  2:472358/1‑140 (MQ=255)                      gATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGGAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAgctgct                                                                                                              >  2:564584/1‑140 (MQ=255)                      gATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAgctgct                                                                                                              >  2:116823/1‑140 (MQ=255)                      gATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAgctgct                                                                                                              >  2:151427/1‑140 (MQ=255)                            gACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTAACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCtt                                                                                                        >  1:1034837/1‑140 (MQ=255)                                aTGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTAcc                                                                                                    >  2:903717/1‑140 (MQ=255)                                   cgcTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCAt                                                                                                 <  1:802001/140‑1 (MQ=255)                                        gTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGtt                                                                                            >  2:547077/1‑140 (MQ=255)                                            aGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATg                                                                                        <  2:687537/140‑1 (MQ=255)                                             gATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATgg                                                                                       >  1:304411/1‑140 (MQ=255)                                                 gACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCtt                                                                                   <  2:737320/140‑1 (MQ=255)                                                  aCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTc                                                                                  >  1:741564/1‑140 (MQ=255)                                                  aCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTc                                                                                  >  1:88859/1‑140 (MQ=255)                                                   cGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCg                                                                                 >  1:959987/1‑140 (MQ=255)                                                   cGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCg                                                                                 >  2:276945/1‑140 (MQ=255)                                                       cAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGt                                                                             <  1:151427/140‑1 (MQ=255)                                                       cAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGt                                                                             <  2:1088781/140‑1 (MQ=255)                                                        aGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGtt                                                                            >  2:457082/1‑140 (MQ=255)                                                          gCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTcc                                                                          >  1:1075568/1‑140 (MQ=255)                                                          gCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTcc                                                                          >  1:430339/1‑140 (MQ=255)                                                          gCCGTGATTCTGGCGAAAGATCTGGTGGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTcc                                                                          >  2:783994/1‑140 (MQ=255)                                                               gATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCaa                                                                     <  1:1031175/140‑1 (MQ=255)                                                                   cTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCt                                                                 <  2:1133406/140‑1 (MQ=255)                                                                   cTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCt                                                                 <  2:324256/140‑1 (MQ=255)                                                                    tGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCCTTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc                                                                >  1:10345/1‑140 (MQ=255)                                                                     ggCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCg                                                               <  1:531843/140‑1 (MQ=255)                                                                         aaaGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGa                                                           <  2:457786/140‑1 (MQ=255)                                                                         aaaGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGa                                                           <  1:717586/140‑1 (MQ=255)                                                                         aaaGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGa                                                           <  1:116823/140‑1 (MQ=255)                                                                              tCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACg                                                      <  1:547077/140‑1 (MQ=255)                                                                               cTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGt                                                     <  2:741564/140‑1 (MQ=255)                                                                                 ggTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTa                                                   <  1:585807/140‑1 (MQ=255)                                                                                      aaaGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTg                                              <  1:564584/140‑1 (MQ=255)                                                                                         gCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGAt                                           >  1:324505/1‑140 (MQ=255)                                                                                         gCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGAt                                           <  1:783994/140‑1 (MQ=255)                                                                                            tAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGcc                                        >  2:451155/1‑140 (MQ=255)                                                                                            tAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGcc                                        >  1:149982/1‑140 (MQ=255)                                                                                               tGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGGGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGt                                     >  2:407318/1‑140 (MQ=255)                                                                                                    cGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCgg                                >  1:773975/1‑140 (MQ=255)                                                                                                    cGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCg                                 >  2:480631/1‑139 (MQ=255)                                                                                                    cGTATCGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCg                                 <  1:480631/139‑1 (MQ=255)                                                                                                         cGGCGTGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCg                           >  2:181399/1‑140 (MQ=255)                                                                                                              tGACCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTcac                      >  2:11922/1‑140 (MQ=255)                                                                                                                aCCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCacac                    <  1:472358/140‑1 (MQ=255)                                                                                                                aCCGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGACAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCacac                    <  2:441959/140‑1 (MQ=255)                                                                                                                aCCGATTACACCATTCTCGGCGCGGT‑AAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCAATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTc                        >  2:478994/1‑135 (MQ=255)                                                                                                                aCCGATTACACCATTCTCGGCGCGGT‑AAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCAATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTc                        <  1:478994/135‑1 (MQ=255)                                                                                                                 ccGATTACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATTCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACAcc                   >  2:665604/1‑140 (MQ=255)                                                                                                                     ttACACCATTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCgcgc               >  1:1055730/1‑140 (MQ=255)                                                                                                                            aTTCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCa        <  2:818712/140‑1 (MQ=255)                                                                                                                             ttCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCAc       >  1:446968/1‑140 (MQ=255)                                                                                                                             ttCTCGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCAc       >  1:386520/1‑140 (MQ=255)                                                                                                                               ctcGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACgg     >  1:85339/1‑140 (MQ=255)                                                                                                                               ctcGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACgg     >  1:584827/1‑140 (MQ=255)                                                                                                                                tcGGCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGc    >  2:991653/1‑140 (MQ=255)                                                                                                                                  ggCGCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGccc  <  2:959987/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCC  >  NC_000913/23141‑23409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑