Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,735,938	G→A	A25V (GCC→GTC)	rluD ←	23S rRNA pseudouridine(1911/1915/1917) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,735,938	0	G	A	100.0%	344.7 / NA	97	A25V (GCC→GTC)	rluD	23S rRNA pseudouridine(1911/1915/1917) synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/64);  total (33/64)

TCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGGCCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTGCCGTTTAATATAGTGTG  >  NC_000913/2735801‑2736070                                                                                                                                          |                                                                                                                                     tctcAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGAcc                                                                                                                                    <  1:85228/140‑1 (MQ=255)     aGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAg                                                                                                                                >  2:1093882/1‑140 (MQ=255)     aGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAg                                                                                                                                >  1:481822/1‑140 (MQ=255)     aGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAg                                                                                                                                >  1:358107/1‑140 (MQ=255)      gCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGc                                                                                                                               <  2:839741/140‑1 (MQ=255)      gCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGc                                                                                                                               <  2:656955/140‑1 (MQ=255)      gCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGc                                                                                                                               <  1:372156/140‑1 (MQ=255)       cGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGcc                                                                                                                              <  1:676088/140‑1 (MQ=255)       cGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGcc                                                                                                                              <  2:646427/140‑1 (MQ=255)       cGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGcc                                                                                                                              <  1:419755/140‑1 (MQ=255)        gTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCt                                                                                                                             <  2:481822/140‑1 (MQ=255)        gTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCt                                                                                                                             <  2:240505/140‑1 (MQ=255)           gATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGc                                                                                                                               >  1:667238/1‑135 (MQ=255)           gATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGc                                                                                                                               <  2:667238/135‑1 (MQ=255)           gATGGCAACCTCCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGAt                                                                                                                          >  2:471238/1‑140 (MQ=255)                  aCCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCg                                                                                                                   <  2:1112009/140‑1 (MQ=255)                      gCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGa                                                                                                               >  2:755960/1‑140 (MQ=255)                        tccccccccAATTCCTTTTCCTTCCGCCTAACACAAACCTTTGCGTTAACCCACCCCCGCTGGTCGGAGGTTCATTTCTTTAATCGCGAAAGGGAAAAATCCCGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGAcc                                                                                                             <  2:832417/134‑1 (MQ=255)                            cACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGt                                                                                                         >  2:909756/1‑140 (MQ=255)                              cccAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTg                                                                                                       >  2:294329/1‑140 (MQ=255)                                 aaTACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGtt                                                                                                    >  1:430875/1‑140 (MQ=255)                                       ttttCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGa                                                                                              <  1:755960/140‑1 (MQ=255)                                       ttttCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGa                                                                                              <  2:185867/140‑1 (MQ=255)                                       ttttCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGa                                                                                              <  1:137275/140‑1 (MQ=255)                                        tttctttcGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGac                                                                                             >  2:371636/1‑140 (MQ=255)                                        tttctttcGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGa                                                                                              >  1:829926/1‑139 (MQ=255)                                        tttctttcGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGa                                                                                              <  2:829926/139‑1 (MQ=255)                                                 gCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCa                                                                                    <  1:947344/140‑1 (MQ=255)                                                 gCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCa                                                                                    <  2:1054045/140‑1 (MQ=255)                                                     aTCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGa                                                                                <  1:363615/140‑1 (MQ=255)                                                     aTCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGa                                                                                >  2:134583/1‑140 (MQ=255)                                                      tCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAg                                                                               <  1:909756/140‑1 (MQ=255)                                                      tCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAg                                                                               <  1:385279/140‑1 (MQ=255)                                                           aaCTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTa                                                                          <  1:241113/140‑1 (MQ=255)                                                            aCTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTTATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTAc                                                                         <  1:463/140‑1 (MQ=255)                                                               ttGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCg                                                                      <  1:970249/140‑1 (MQ=255)                                                                tGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGt                                                                     >  1:1040194/1‑140 (MQ=255)                                                                tGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGt                                                                     >  1:857531/1‑140 (MQ=255)                                                                tGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGt                                                                     >  1:129559/1‑140 (MQ=255)                                                                tGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGt                                                                     >  2:730020/1‑140 (MQ=255)                                                                tGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGt                                                                     >  1:660907/1‑140 (MQ=255)                                                                 gCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGtt                                                                    <  2:545171/140‑1 (MQ=255)                                                                 gCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGtt                                                                    <  2:162740/140‑1 (MQ=255)                                                                 gCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGtt                                                                    <  1:264618/140‑1 (MQ=255)                                                                 gCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGtt                                                                    <  1:471238/140‑1 (MQ=255)                                                                 gCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGtt                                                                    <  1:371636/140‑1 (MQ=255)                                                                   cGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTtgt                                                                  <  1:311356/140‑1 (MQ=255)                                                                   cGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTtgt                                                                  <  2:857531/140‑1 (MQ=255)                                                                   cGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTtgt                                                                  <  2:1040194/140‑1 (MQ=255)                                                                   cGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTtgt                                                                  <  2:818278/140‑1 (MQ=255)                                                                   cGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTtgt                                                                  <  1:413068/140‑1 (MQ=255)                                                                     ttAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGtt                                                                    >  1:1114918/1‑136 (MQ=255)                                                                     ttAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGtt                                                                    <  2:1114918/136‑1 (MQ=255)                                                                     ttAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGc                                                                <  1:134583/140‑1 (MQ=255)                                                                      tAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGcc                                                               <  1:527348/140‑1 (MQ=255)                                                                         ccAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCata                                                            <  2:427322/140‑1 (MQ=255)                                                                            gCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGtt                                                                    >  2:247607/1‑129 (MQ=255)                                                                            gCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGtt                                                                    <  1:247607/129‑1 (MQ=255)                                                                             cACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTa                                                                          <  1:57573/122‑1 (MQ=255)                                                                             cACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTa                                                                          >  2:57573/1‑122 (MQ=255)                                                                             cACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCAtatata                                                        <  1:730020/140‑1 (MQ=255)                                                                                  gCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGcc                                                               >  2:987482/1‑128 (MQ=255)                                                                                  gCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGcc                                                               <  1:987482/128‑1 (MQ=255)                                                                                  gCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGct                                                   >  1:337841/1‑140 (MQ=255)                                                                                     ggTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGcttct                                                <  1:643074/140‑1 (MQ=255)                                                                                     ggTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGcttct                                                >  1:649957/1‑140 (MQ=255)                                                                                      gTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGcttctt                                               >  2:126081/1‑140 (MQ=255)                                                                                       tCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCttcttc                                              >  1:507233/1‑140 (MQ=255)                                                                                              tCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATc                                       >  1:1071041/1‑140 (MQ=255)                                                                                              tCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATc                                       >  1:492735/1‑140 (MQ=255)                                                                                                cATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGt                                     <  2:492735/140‑1 (MQ=255)                                                                                                          ttCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTa                           >  1:732011/1‑140 (MQ=255)                                                                                                          ttCGCGAACGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTa                           >  1:485167/1‑140 (MQ=255)                                                                                                                  cGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   <  1:849764/140‑1 (MQ=255)                                                                                                                  cGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   <  1:312807/140‑1 (MQ=255)                                                                                                                  cGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   <  2:1071041/140‑1 (MQ=255)                                                                                                                  cGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   <  2:507233/140‑1 (MQ=255)                                                                                                                  cGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   <  1:201929/140‑1 (MQ=255)                                                                                                                  cGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   <  1:852000/140‑1 (MQ=255)                                                                                                                  cGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   <  2:337841/140‑1 (MQ=255)                                                                                                                  cGTGAATAATCCGGGAACATTTCGACCAAAGCCTGATCTAAGAGTTGACCGAGTTGGTTTTCGGACACCGTTGCACTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   <  2:1055707/140‑1 (MQ=255)                                                                                                                      aataatCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTGCCGt               <  2:649957/140‑1 (MQ=255)                                                                                                                       ataatCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTGCCGtt              <  1:294329/140‑1 (MQ=255)                                                                                                                           tCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   >  1:620143/1‑131 (MQ=255)                                                                                                                           tCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   <  2:620143/131‑1 (MQ=255)                                                                                                                           tCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTGCCGTTTAat          <  1:1093882/140‑1 (MQ=255)                                                                                                                           tCCGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTGCCGTTTAat          >  1:582459/1‑140 (MQ=255)                                                                                                                            ccGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTGCCGTTTAata         <  2:732011/140‑1 (MQ=255)                                                                                                                            ccGGGAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTGCCGTTTAata         <  1:193638/140‑1 (MQ=255)                                                                                                                              gggAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTGCCGTTTAAtata       <  1:1089881/140‑1 (MQ=255)                                                                                                                                gAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   >  1:872809/1‑126 (MQ=255)                                                                                                                                gAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   >  1:911854/1‑126 (MQ=255)                                                                                                                                gAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   <  2:872809/126‑1 (MQ=255)                                                                                                                                gAACATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTg                   <  2:911854/126‑1 (MQ=255)                                                                                                                                  aCATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTGCCGTTTAATATAgtgt   <  2:430875/140‑1 (MQ=255)                                                                                                                                  aCATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTGCCGTTTAATATAgtgt   <  1:126081/140‑1 (MQ=255)                                                                                                                                   cATTTCGACCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTGCCGTTTAATATAGtgtg  <  2:129559/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     TCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGAACGTGAATAATCCGGGAACATTTCGGCCAAAGCCTGATCTAAGCGTTGACCGAGTTGGTTTTCGGACACCGTTGCAGTGAGCTGTACTCGTTGTGCCATATATACTGCTTCTTCGTTTATCGTTGGGGTTTTACGGCTTTGCCGTTTAATATAGTGTG  >  NC_000913/2735801‑2736070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑