Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,749,698	T→C	Y309H (TAT→CAT)	yfjD →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,749,698	0	T	C	100.0%	101.0 / NA	28	Y309H (TAT→CAT)	yfjD	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/17);  total (11/17)

AAAAGAGTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGG  >  NC_000913/2749565‑2749831                                                                                                                                      |                                                                                                                                      aaaaGAGTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGgag                                                                                                                                 <  2:254508/140‑1 (MQ=255)  aaaGAGTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGgaga                                                                                                                                <  2:1102727/140‑1 (MQ=255)  aaaGAGTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGgaga                                                                                                                                <  1:1111509/140‑1 (MQ=255)        ttCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCa                                                                                                                          <  2:530687/140‑1 (MQ=255)            ccAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAgggg                                                                                                                      <  2:887672/140‑1 (MQ=255)                 gAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGt                                                                                                                 <  1:136843/140‑1 (MQ=255)                             cgcgcCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGa                                                                                                     >  1:486881/1‑140 (MQ=255)                             cgcgcCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGa                                                                                                     <  1:864442/140‑1 (MQ=255)                               cgccgcGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGata                                                                                                   >  2:607055/1‑140 (MQ=255)                                 ccgcGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATAtt                                                                                                 <  1:1029886/140‑1 (MQ=255)                                  cgcgGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTc                                                                                                >  1:890393/1‑140 (MQ=255)                                            aTCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAag                                                                                          <  2:591856/136‑1 (MQ=255)                                            aTCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAag                                                                                          >  1:591856/1‑136 (MQ=255)                                                     gtgCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGa                                                                             >  1:739852/1‑140 (MQ=255)                                                     gtgCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGa                                                                             <  1:607055/140‑1 (MQ=255)                                                          ggAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCa                                                                        <  2:545261/140‑1 (MQ=255)                                                                      gcTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGt                                                            >  1:1119307/1‑140 (MQ=255)                                                                       cTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTc                                                           >  1:928747/1‑140 (MQ=255)                                                                             aCGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAac                                                     <  2:486881/140‑1 (MQ=255)                                                                                           gTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAgg                                       >  2:1013551/1‑140 (MQ=255)                                                                                             ttCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTc                                     >  1:330467/1‑140 (MQ=255)                                                                                                      acaaAAAGAAAGTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCaa                            <  1:1013551/140‑1 (MQ=255)                                                                                                                agTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGtt                  <  2:319654/140‑1 (MQ=255)                                                                                                                 gTCGGCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTc                 <  2:890393/140‑1 (MQ=255)                                                                                                                     gCCTGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTg             >  2:668948/1‑140 (MQ=255)                                                                                                                        tGGTCGTCAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGAtt          <  1:406250/140‑1 (MQ=255)                                                                                                                               cAACGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATg   >  1:967116/1‑140 (MQ=255)                                                                                                                                aaCGAGCATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATgg  <  1:668948/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      AAAAGAGTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGG  >  NC_000913/2749565‑2749831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑