Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,793,716	G→A	G328R (GGG→AGG)	gabT →	4‑aminobutyrate aminotransferase GabT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,793,716	0	G	A	100.0%	183.4 / NA	52	G328R (GGG→AGG)	gabT	4‑aminobutyrate aminotransferase GabT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/24);  total (28/24)

CGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAA  >  NC_000913/2793582‑2793847                                                                                                                                       |                                                                                                                                    cgGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAg                                                                                                                                <  2:272567/140‑1 (MQ=255)  ggAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGa                                                                                                                               <  2:1009236/140‑1 (MQ=255)        aaTGGATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGa                                                                                                                         <  1:303834/140‑1 (MQ=255)              tGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACg                                                                                                                   >  1:604674/1‑140 (MQ=255)              tGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACg                                                                                                                   <  1:980519/140‑1 (MQ=255)              tGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAATTGAAAGACg                                                                                                                   >  1:779878/1‑140 (MQ=255)               gCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACgg                                                                                                                  >  1:416891/1‑140 (MQ=255)                ccGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGa                                                                                                                 >  2:935456/1‑140 (MQ=255)                ccGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGa                                                                                                                 >  2:615040/1‑140 (MQ=255)                 cGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGAt                                                                                                                >  1:847318/1‑140 (MQ=255)                 cGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGAt                                                                                                                >  2:572062/1‑140 (MQ=255)                           ggCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGAt                                                                                                      >  1:677101/1‑140 (MQ=255)                                     gcggcACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGaaaaa                                                                                            <  1:572062/140‑1 (MQ=255)                                      cggcACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAac                                                                                           >  2:834720/1‑140 (MQ=255)                                         cACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACAcc                                                                                        >  1:500718/1‑140 (MQ=255)                                           ccTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCg                                                                                      <  2:500718/140‑1 (MQ=255)                                             tATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGga                                                                                    <  2:630208/140‑1 (MQ=255)                                                 cGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATc                                                                                <  1:415267/140‑1 (MQ=255)                                                   ggTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAacac                                                                                         <  1:777786/129‑1 (MQ=255)                                                   ggTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAacac                                                                                         >  2:777786/1‑129 (MQ=255)                                                      aaCCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGa                                                                           <  2:604674/140‑1 (MQ=255)                                                      aaCCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGa                                                                           >  1:1133247/1‑140 (MQ=255)                                                        cccGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACg                                                                         >  2:514476/1‑140 (MQ=255)                                                            aTTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcg                                                                     <  2:1133247/140‑1 (MQ=255)                                                            aTTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcg                                                                     <  2:3284/140‑1 (MQ=255)                                                            aTTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcg                                                                     <  1:834720/140‑1 (MQ=255)                                                            aTTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcg                                                                     <  1:359222/140‑1 (MQ=255)                                                            aTTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcg                                                                     <  2:847318/140‑1 (MQ=255)                                                            aTTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcg                                                                     <  2:877969/140‑1 (MQ=255)                                                             ttGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcgc                                                                    >  1:71337/1‑140 (MQ=255)                                                                ccTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCggg                                                                 >  1:1083670/1‑140 (MQ=255)                                                                  tGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCt                                                               <  1:935456/140‑1 (MQ=255)                                                                   gCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTg                                                              >  1:1087259/1‑140 (MQ=255)                                                                           gcgcTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCgat                                                      <  2:416891/140‑1 (MQ=255)                                                                              cTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCgatgat                                                   >  2:949085/1‑140 (MQ=255)                                                                              cTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCgatgat                                                   <  2:864007/140‑1 (MQ=255)                                                                                           aGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAg                                         <  1:471442/137‑1 (MQ=255)                                                                                           aGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAg                                         >  2:471442/1‑137 (MQ=255)                                                                                           aGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTg                                      >  1:179596/1‑140 (MQ=255)                                                                                            ggTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGt                                     >  1:364926/1‑140 (MQ=255)                                                                                                tttGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGa                                 <  2:1087259/140‑1 (MQ=255)                                                                                                 ttGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGaa                                >  1:459609/1‑140 (MQ=255)                                                                                                      cAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACgg                           <  1:188913/140‑1 (MQ=255)                                                                                                       aGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGc                          >  2:262359/1‑140 (MQ=255)                                                                                                         gAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGa                        >  2:1003285/1‑140 (MQ=255)                                                                                                          aaaaTCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGAt                       >  1:729417/1‑140 (MQ=255)                                                                                                                  ctgcAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCAcaacaa               >  1:408313/1‑140 (MQ=255)                                                                                                                   tgcAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGAGATCACAACAAg              >  1:1105706/1‑140 (MQ=255)                                                                                                                       aaaaGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCgg          <  1:949085/140‑1 (MQ=255)                                                                                                                          aGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACg       >  1:762135/1‑140 (MQ=255)                                                                                                                            ccAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGcc     <  1:257617/140‑1 (MQ=255)                                                                                                                               aCGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGTTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCaaa  >  2:237110/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                    CGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAA  >  NC_000913/2793582‑2793847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑