Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,828,224	G→A	G163E (GGG→GAG)	srlD →	sorbitol‑6‑phosphate 2‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,828,224	0	G	A	100.0%	302.8 / NA	85	G163E (GGG→GAG)	srlD	sorbitol‑6‑phosphate 2‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/40);  total (45/40)

CTGTGTGCGCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAA  >  NC_000913/2828088‑2828357                                                                                                                                         |                                                                                                                                      cTGTGTGCGCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCt                                                                                                                                    <  2:641792/140‑1 (MQ=255)  tgtgtgCGCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTg                                                                                                                                   <  1:507583/140‑1 (MQ=255)  tgtgtgCGCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTg                                                                                                                                   >  1:836970/1‑140 (MQ=255)    tgtgCGCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGAc                                                                                                                                 >  2:895339/1‑140 (MQ=255)    tgtgCGCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGAc                                                                                                                                 >  1:215355/1‑140 (MQ=255)      tgCGCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTc                                                                                                                               >  2:212388/1‑140 (MQ=255)        cgcgTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCaa                                                                                                                             >  2:103790/1‑140 (MQ=255)        cgcgTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCaa                                                                                                                             >  1:179088/1‑140 (MQ=255)        cgcgTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCaa                                                                                                                             >  2:746319/1‑140 (MQ=255)        cgcgTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCaa                                                                                                                             >  2:764606/1‑140 (MQ=255)         gcgTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAAt                                                                                                                            >  2:1099372/1‑140 (MQ=255)          cgTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATc                                                                                                                           >  2:840572/1‑140 (MQ=255)          cgTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATc                                                                                                                           >  1:667397/1‑140 (MQ=255)            tGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCAc                                                                                                                         <  1:212388/140‑1 (MQ=255)            tGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCAc                                                                                                                         <  1:1125447/140‑1 (MQ=255)              aaTTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCAc                                                                                                                         >  1:493748/1‑138 (MQ=255)              aaTTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCAc                                                                                                                         <  2:493748/138‑1 (MQ=255)                      cgTTTAATGATCGGCGACGGGATTCAGGGGCGCATTATTAAGATAACCTCGAAACCCGGC‑AAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGAt                                                                                                              <  1:1111394/140‑1 (MQ=255)                        tttGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATc                                                                                                             <  2:812605/140‑1 (MQ=255)                          tgatgaTCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTg                                                                                                           >  1:931835/1‑140 (MQ=255)                           gatgatCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTgg                                                                                                          <  1:103790/140‑1 (MQ=255)                                 ccGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGCGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGt                                                                                                    >  1:100604/1‑140 (MQ=255)                                  cgcgACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTa                                                                                                   <  1:734162/140‑1 (MQ=255)                                  cgcgACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTa                                                                                                   <  2:575589/140‑1 (MQ=255)                                   gcgACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCt                                                                                                                  <  1:562075/124‑1 (MQ=255)                                   gcgACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCt                                                                                                                  >  2:562075/1‑124 (MQ=255)                                      aCGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGc                                                                                               >  2:555902/1‑140 (MQ=255)                                       cGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCa                                                                                              >  2:307306/1‑140 (MQ=255)                                       cGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCa                                                                                              <  2:995968/140‑1 (MQ=255)                                        gggATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCAt                                                                                             <  2:179088/140‑1 (MQ=255)                                          gATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTa                                                                                           >  2:739614/1‑140 (MQ=255)                                           aTTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTAc                                                                                          <  1:746319/140‑1 (MQ=255)                                           aTTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTAc                                                                                          <  1:840572/140‑1 (MQ=255)                                             tCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACgg                                                                                        <  1:307306/140‑1 (MQ=255)                                              cAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGt                                                                                       <  2:931835/140‑1 (MQ=255)                                               aGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTAc                                                                                          <  1:213851/136‑1 (MQ=255)                                               aGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTAc                                                                                          >  2:213851/1‑136 (MQ=255)                                                     gcATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCa                                                                                >  2:1093142/1‑140 (MQ=255)                                                          attCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGAt                                                                           <  2:215355/140‑1 (MQ=255)                                                                aTCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGa                                                                            >  2:296705/1‑133 (MQ=255)                                                                aTCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGa                                                                            <  1:296705/133‑1 (MQ=255)                                                                aTCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCgg                                                                     >  2:443113/1‑140 (MQ=255)                                                                aTCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCgg                                                                     >  2:337377/1‑140 (MQ=255)                                                                 tCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGt                                                                    >  1:503395/1‑140 (MQ=255)                                                                 tCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGCGCATTCACTGATGCTCGGt                                                                    >  2:1025365/1‑140 (MQ=255)                                                                  cAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTa                                                                   <  1:337377/140‑1 (MQ=255)                                                                     cTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAAcc                                                                >  2:53613/1‑140 (MQ=255)                                                                     cTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAAcc                                                                >  2:149800/1‑140 (MQ=255)                                                                     cTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAAcc                                                                >  2:724164/1‑140 (MQ=255)                                                                     cTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAAcc                                                                >  1:238643/1‑140 (MQ=255)                                                                     cTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAAcc                                                                >  1:852879/1‑140 (MQ=255)                                                                     cTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAAcc                                                                >  1:640271/1‑140 (MQ=255)                                                                              cGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAAt                                                       <  2:667397/140‑1 (MQ=255)                                                                                gCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCg                                                     >  2:112988/1‑140 (MQ=255)                                                                                   aaGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCg                                                  <  1:112988/140‑1 (MQ=255)                                                                                   aaGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCg                                                  <  1:764606/140‑1 (MQ=255)                                                                                       gggCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGt                                              <  1:443113/140‑1 (MQ=255)                                                                                           agcaAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATg                                               <  1:859518/135‑1 (MQ=255)                                                                                           agcaAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATg                                               >  2:859518/1‑135 (MQ=255)                                                                                             caAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGt                                        >  1:781563/1‑140 (MQ=255)                                                                                              aaaCACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTc                                       <  1:739614/140‑1 (MQ=255)                                                                                               aaCACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCa                                      <  1:1093142/140‑1 (MQ=255)                                                                                                  acaACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTg                                   <  2:503395/140‑1 (MQ=255)                                                                                                   caACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGt                                  <  2:424140/140‑1 (MQ=255)                                                                                                              aCAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCca                             <  1:836897/134‑1 (MQ=255)                                                                                                              aCAGCGCAGCGAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCca                             >  2:836897/1‑134 (MQ=255)                                                                                                                agcgcagcgAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATAcgc                     >  2:553441/1‑140 (MQ=255)                                                                                                                  cgcagcgAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGa                   >  2:910017/1‑140 (MQ=255)                                                                                                                  cgcagcgAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGa                   >  2:1009894/1‑140 (MQ=255)                                                                                                                  cgcagcgAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGa                   >  1:552935/1‑140 (MQ=255)                                                                                                                   gcagcgAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGAc                  <  1:696250/140‑1 (MQ=255)                                                                                                                     agcgAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCa                >  1:269368/1‑140 (MQ=255)                                                                                                                     agcgAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCa                >  2:747124/1‑140 (MQ=255)                                                                                                                     agcgAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCa                >  1:509891/1‑140 (MQ=255)                                                                                                                     agcgAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCa                <  2:552935/140‑1 (MQ=255)                                                                                                                     agcgAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGAACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCa                >  2:557969/1‑140 (MQ=255)                                                                                                                      gcgAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCaa               <  1:1099372/140‑1 (MQ=255)                                                                                                                       cgAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAg              >  2:710448/1‑140 (MQ=255)                                                                                                                       cgAAATTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGAACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAg              <  1:557969/140‑1 (MQ=255)                                                                                                                         aaaTTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCt            <  2:1132029/140‑1 (MQ=255)                                                                                                                          aaTTTGGTGGCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTg           <  2:238643/140‑1 (MQ=255)                                                                                                                               ggtggCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTAt      <  1:747124/140‑1 (MQ=255)                                                                                                                                   gCGTCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCaaa  <  1:555902/140‑1 (MQ=255)                                                                                                                                      tCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAGCCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCt            <  1:563517/127‑1 (MQ=255)                                                                                                                                      tCGAGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAGCCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCt            >  2:563517/1‑127 (MQ=255)                                                                                                                                         |                                                                                                                                      CTGTGTGCGCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAA  >  NC_000913/2828088‑2828357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑