Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,937,868	T→C	F134S (TTC→TCC)	fucK →	L‑fuculokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,937,868	0	T	C	100.0%	216.0 / NA	57	F134S (TTC→TCC)	fucK	L‑fuculokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/34);  total (23/34)

AGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCACTAC  >  NC_000913/2937734‑2937988                                                                                                                                       |                                                                                                                         aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATa                                                                                                                     <  2:970669/140‑1 (MQ=255) aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATa                                                                                                                     <  2:842641/140‑1 (MQ=255) aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATa                                                                                                                     <  2:218017/140‑1 (MQ=255)  gATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATAc                                                                                                                    <  2:706900/140‑1 (MQ=255)   aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACg                                                                                                                   <  1:697106/140‑1 (MQ=255)       gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTtat                                                                                                               <  1:982517/140‑1 (MQ=255)       gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTtat                                                                                                               <  1:535940/140‑1 (MQ=255)       gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGCTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTtat                                                                                                               <  2:839255/140‑1 (MQ=255)        cAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTtata                                                                                                              <  1:858238/140‑1 (MQ=255)         aaGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATa                                                                                                                     <  1:966263/132‑1 (MQ=255)         aaGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATa                                                                                                                     >  2:966263/1‑132 (MQ=255)         aaGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTtatat                                                                                                             >  2:450668/1‑140 (MQ=255)            gCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAg                                                                                                          <  1:251053/140‑1 (MQ=255)                tCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCaa                                                                                                                       >  2:652973/1‑123 (MQ=255)                tCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCaa                                                                                                                       <  1:652973/123‑1 (MQ=255)                     tctATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATATGTTGGTGTgg                                                                                                 <  2:535903/140‑1 (MQ=255)                      ctATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGt                                                                                                <  1:591881/140‑1 (MQ=255)                      ctATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGt                                                                                                <  1:1076505/140‑1 (MQ=255)                            gATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTgaaag                                                                                          >  2:648661/1‑140 (MQ=255)                              ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTgaaagaa                                                                                        >  2:818137/1‑140 (MQ=255)                              ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTgaaagaa                                                                                        >  2:926928/1‑140 (MQ=255)                              ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTgaaagaa                                                                                        >  1:200091/1‑140 (MQ=255)                                   aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAatca                                                                                   >  2:989689/1‑140 (MQ=255)                                   aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAatca                                                                                   >  1:704164/1‑140 (MQ=255)                                   aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAatca                                                                                   >  1:266535/1‑140 (MQ=255)                                        gAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                                                                              <  1:573962/140‑1 (MQ=255)                                        gAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                                                                              <  2:272215/140‑1 (MQ=255)                                        gAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                                                                              <  1:450668/140‑1 (MQ=255)                                              tCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAActgc                                                                        >  2:1090146/1‑140 (MQ=255)                                                cgcgAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAActgctg                                                                      >  1:384152/1‑140 (MQ=255)                                                   gAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACaa                                                                            >  1:501006/1‑131 (MQ=255)                                                   gAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACaa                                                                            <  2:501006/131‑1 (MQ=255)                                                    aaCAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAc                                                                  <  2:200091/140‑1 (MQ=255)                                                         cagcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgcgcg                                                             <  2:678375/140‑1 (MQ=255)                                                         cagcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgcgcg                                                             <  1:534194/140‑1 (MQ=255)                                                         cagcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgcgcg                                                             <  2:576283/140‑1 (MQ=255)                                                         cagcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgcgcg                                                             <  2:266535/140‑1 (MQ=255)                                                              gTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGc                                                        <  1:989689/140‑1 (MQ=255)                                                                 aTGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTg                                                     <  2:704164/140‑1 (MQ=255)                                                                         tatTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgcgcg                                                             >  2:813004/1‑124 (MQ=255)                                                                         tatTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgcgcg                                                             <  1:813004/124‑1 (MQ=255)                                                                         tatTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTa                                             >  1:1079571/1‑140 (MQ=255)                                                                         tatTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTa                                             >  2:105982/1‑140 (MQ=255)                                                                           ttGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTAtt                                           <  1:648661/140‑1 (MQ=255)                                                                                 ggTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTtcgtcg                                     <  2:596875/140‑1 (MQ=255)                                                                                   ttAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCt                                   >  1:91653/1‑140 (MQ=255)                                                                                   ttAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCt                                   >  1:1058486/1‑140 (MQ=255)                                                                                   ttAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCt                                   >  1:634102/1‑140 (MQ=255)                                                                                          ccGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAac                            <  2:389893/140‑1 (MQ=255)                                                                                           cGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAacc                           >  1:221853/1‑140 (MQ=255)                                                                                             cacaGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAaccac                         >  2:335621/1‑140 (MQ=255)                                                                                                 gCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGtt                     >  1:674650/1‑140 (MQ=255)                                                                                                    gTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTaa                  <  1:335621/140‑1 (MQ=255)                                                                                                             tATTTCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAAt         <  2:384152/140‑1 (MQ=255)                                                                                                               tttCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGa           >  2:982045/1‑136 (MQ=255)                                                                                                               tttCTGGCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGa           <  1:982045/136‑1 (MQ=255)                                                                                                                    ggCGTCGGAGCCTTTAGTTCCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCactac  <  2:1079571/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                         AGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCACTAC  >  NC_000913/2937734‑2937988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑