| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,950,940 | T→C | T508A (ACG→GCG) | recD ← | exodeoxyribonuclease V subunit RecD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,950,940 | 0 | T | C | 97.5% | 138.7 / ‑4.7 | 40 | T508A (ACG→GCG) | recD | exodeoxyribonuclease V subunit RecD |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (20/19); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAATCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTG > NC_000913/2950804‑2951072 | aaaaTCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGcccc < 2:1007695/140‑1 (MQ=255)aaaaTCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGcccc < 2:103224/140‑1 (MQ=255)aaaaTCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGcccc < 1:55758/140‑1 (MQ=255) aaTCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCt < 1:1110706/140‑1 (MQ=255) gcATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATc < 1:485723/140‑1 (MQ=255) gcATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATc < 2:685918/140‑1 (MQ=255) tCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAgcgc < 2:868953/140‑1 (MQ=255) aCTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATa < 1:1092779/140‑1 (MQ=255) aTCCCTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGACCCAGCGCAATACCGata > 2:165786/1‑140 (MQ=255) tCCCTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGatat < 1:1030985/140‑1 (MQ=255) tCCCTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGatat < 1:516018/140‑1 (MQ=255) ccTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGGACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCg > 2:1014620/1‑140 (MQ=255) cTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGc < 2:658477/140‑1 (MQ=255) gCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCa < 2:479107/140‑1 (MQ=255) gTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAg > 1:100153/1‑140 (MQ=255) gTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAg > 2:765643/1‑140 (MQ=255) gTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAg > 1:286135/1‑140 (MQ=255) cATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAgcg < 1:552612/140‑1 (MQ=255) tCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAgcgcg < 1:765643/140‑1 (MQ=255) cGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCAt > 1:55682/1‑140 (MQ=255) cGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCAt > 2:1048590/1‑140 (MQ=255) cGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCAt > 1:679881/1‑140 (MQ=255) gCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAatc > 1:102286/1‑140 (MQ=255) aCTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGAc < 2:100153/140‑1 (MQ=255) cTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGAcc > 2:684626/1‑140 (MQ=255) tCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCt < 1:684626/140‑1 (MQ=255) tCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCt < 2:55682/140‑1 (MQ=255) tCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCt < 2:679881/140‑1 (MQ=255) ttGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGt < 1:77341/140‑1 (MQ=255) aGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAAc < 2:1103939/140‑1 (MQ=255) aCTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACga > 1:621251/1‑140 (MQ=255) gCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGAt > 1:909794/1‑140 (MQ=255) gCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGAt > 1:772795/1‑140 (MQ=255) gCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGAt > 1:580691/1‑140 (MQ=255) gCCGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGAt > 1:379936/1‑140 (MQ=255) ccGTCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGTGGATg > 1:735362/1‑140 (MQ=255) ccGTCCGGCATCACAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGCTCTCGCCATTAAATAACCCCAGTGCGCTGTCATTACGGGCAATCATCACCGGACGACCTTCGTACTAACGAGAGTGCggcta > 2:790667/1‑137 (MQ=255) tCCGGCATCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACg < 2:580691/140‑1 (MQ=255) tCGCAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAAtt > 2:91401/1‑140 (MQ=255) cAAACCAGACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTg < 1:1014620/140‑1 (MQ=255) gACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCt < 2:286135/140‑1 (MQ=255) gACGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCt > 1:720386/1‑140 (MQ=255) aCGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTg > 2:344865/1‑140 (MQ=255) aCGCGCGCCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTg > 1:545068/1‑140 (MQ=255) | AAAATCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGTAGTTTCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTG > NC_000913/2950804‑2951072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |