Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,148,684	A→G	N263S (AAC→AGC)	gpr →	L‑glyceraldehyde 3‑phosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,148,684	0	A	G	100.0%	275.3 / NA	74	N263S (AAC→AGC)	gpr	L‑glyceraldehyde 3‑phosphate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/38);  total (36/38)

GGCTGTATTGCCTTTACTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAACCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAAC  >  NC_000913/3148548‑3148818                                                                                                                                         |                                                                                                                                       ggCTGTATTGCCTTTACTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCt                                                                                                                                     >  1:386809/1‑140 (MQ=255)   cTGTATTGCCTTTACTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCa                                                                                                                                   <  1:80452/140‑1 (MQ=255)      tATTGCCTTTACTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACa                                                                                                                                >  2:983460/1‑140 (MQ=255)      tATTGCCTTTACTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACa                                                                                                                                >  2:699452/1‑140 (MQ=255)        tcgCCTTTACTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGc                                                                                                                              <  2:795844/138‑1 (MQ=255)           ccTTTACTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTg                                                                                                                           >  2:902786/1‑140 (MQ=255)           ccTTTACTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTg                                                                                                                           >  2:223977/1‑140 (MQ=255)               tACTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCt                                                                                                                       <  2:338999/140‑1 (MQ=255)                aCTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCtt                                                                                                                      >  1:1006219/1‑140 (MQ=255)                aCTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCtt                                                                                                                      >  2:517509/1‑140 (MQ=255)                aCTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCtt                                                                                                                      >  1:1132170/1‑140 (MQ=255)                  tcctcTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTAt                                                                                                                    <  1:1014373/140‑1 (MQ=255)                      ctGGCTCAGGGATTGCTGACCGGAAAATATCTCAATGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATtgaa                                                                                                                >  1:109018/1‑140 (MQ=255)                      ctGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATtgaa                                                                                                                <  2:259355/140‑1 (MQ=255)                      ctGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATtgaa                                                                                                                <  1:517509/140‑1 (MQ=255)                      ctGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATtgaa                                                                                                                <  2:694015/140‑1 (MQ=255)                       tGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATtgaat                                                                                                               >  2:341519/1‑140 (MQ=255)                         gCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATtg                                                                                                                  >  2:403808/1‑135 (MQ=255)                         gCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATtg                                                                                                                  <  1:403808/135‑1 (MQ=255)                           tCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGaaa                                                                                                           <  1:223977/140‑1 (MQ=255)                            cAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTAtt                                                                                                                   <  1:25657/131‑1 (MQ=255)                            cAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTAtt                                                                                                                   >  2:25657/1‑131 (MQ=255)                               ggATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGc                                                                                                       >  1:810032/1‑140 (MQ=255)                                   tGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGCTTCACGGATGCATCGTGAAGGGAATAAAGTACGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCAcag                                                                                                   >  2:150402/1‑140 (MQ=255)                                   tGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCAcag                                                                                                   >  1:352435/1‑140 (MQ=255)                                    gCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCAcagc                                                                                                  >  1:741413/1‑140 (MQ=255)                                      tGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCAcagcag                                                                                                <  2:723066/140‑1 (MQ=255)                                       gACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACagcagc                                                                                               <  1:549690/140‑1 (MQ=255)                                       gACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACagcagc                                                                                               <  1:73892/140‑1 (MQ=255)                                         ccGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGt                                                                                             <  2:386809/140‑1 (MQ=255)                                             aaaaTATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGAc                                                                                         <  2:352435/140‑1 (MQ=255)                                             aaaaTATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGAc                                                                                         >  2:822830/1‑140 (MQ=255)                                                           gCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCaa                                                                           >  2:671515/1‑140 (MQ=255)                                                           gCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCaa                                                                           >  1:354703/1‑140 (MQ=255)                                                            cATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCaaa                                                                          <  2:1132170/140‑1 (MQ=255)                                                            cATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCaaa                                                                          >  2:1077640/1‑140 (MQ=255)                                                                 cGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCg                                                                     >  1:730028/1‑140 (MQ=255)                                                                   cAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGtt                                                                   <  1:83089/140‑1 (MQ=255)                                                                      gATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAg                                                                <  2:741413/140‑1 (MQ=255)                                                                      gATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAg                                                                >  1:288849/1‑140 (MQ=255)                                                                       aTTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGc                                                               <  2:1114277/140‑1 (MQ=255)                                                                         tCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTg                                                             <  1:341519/140‑1 (MQ=255)                                                                          cACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTgg                                                            <  2:801320/140‑1 (MQ=255)                                                                          cACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGATTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGTCAATCAATGGCGCAAATGGCGTTAAGCTgg                                                            <  2:109018/140‑1 (MQ=255)                                                                           aCGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCTAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGt                                                           >  1:122302/1‑140 (MQ=255)                                                                              gATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGc                                                        <  2:288849/140‑1 (MQ=255)                                                                                tGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTg                                                      <  2:354703/140‑1 (MQ=255)                                                                                 gCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGa                                                     >  1:738095/1‑140 (MQ=255)                                                                                  cATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGaa                                                    >  1:102423/1‑140 (MQ=255)                                                                                  cATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGaa                                                    >  2:735084/1‑140 (MQ=255)                                                                                  cATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGaa                                                    >  1:787971/1‑140 (MQ=255)                                                                                      gTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAgat                                                <  1:902786/140‑1 (MQ=255)                                                                                       tGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAgatg                                               >  1:138105/1‑140 (MQ=255)                                                                                              aaTAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAACCAATGGCGCAAATGGCGTTAAGCTGGTTGc                                                        <  1:743798/124‑1 (MQ=255)                                                                                              aaTAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAACCAATGGCGCAAATGGCGTTAAGCTGGTTGc                                                        >  2:743798/1‑124 (MQ=255)                                                                                               aTAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTg                                       >  2:604983/1‑140 (MQ=255)                                                                                                tAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGa                                      >  2:107473/1‑140 (MQ=255)                                                                                                 aaaGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGAc                                     <  2:1119297/140‑1 (MQ=255)                                                                                                  aaGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACg                                    <  1:181567/140‑1 (MQ=255)                                                                                                  aaGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACg                                    <  1:604983/140‑1 (MQ=255)                                                                                                   aGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGt                                   <  2:654391/140‑1 (MQ=255)                                                                                                   aGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGt                                   <  1:822830/140‑1 (MQ=255)                                                                                                    gTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTc                                  <  1:1077640/140‑1 (MQ=255)                                                                                                     ttCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCg                                 <  1:630187/140‑1 (MQ=255)                                                                                                      tCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCgg                                >  1:857575/1‑140 (MQ=255)                                                                                                        gTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTa                              >  1:1069872/1‑140 (MQ=255)                                                                                                        gTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTa                              >  1:588455/1‑140 (MQ=255)                                                                                                        gTGGTCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTa                              <  1:455310/140‑1 (MQ=255)                                                                                                            tCTGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTattga                          >  2:704216/1‑140 (MQ=255)                                                                                                              tGACACCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTattgatt                        <  2:810032/140‑1 (MQ=255)                                                                                                                  acCGAAAATGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTg                    >  2:57917/1‑140 (MQ=255)                                                                                                                      aaaaTGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAg                >  2:655609/1‑140 (MQ=255)                                                                                                                          tGCTTACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCcgc            <  1:107473/140‑1 (MQ=255)                                                                                                                              tACCGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCgg        <  2:787971/140‑1 (MQ=255)                                                                                                                                ccGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAg      <  2:730028/140‑1 (MQ=255)                                                                                                                                 cGAAGCCAGCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGc     <  1:735084/140‑1 (MQ=255)                                                                                                                                    agccagccTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAAc  >  1:861854/1‑140 (MQ=255)                                                                                                                                    agccagccTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAAc  <  1:704216/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GGCTGTATTGCCTTTACTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAACCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAAC  >  NC_000913/3148548‑3148818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑