Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,155,841 T→C F163L (TTC→CTC)  yqhD → NADPH‑dependent aldehyde reductase YqhD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,155,8410TC100.0% 187.9 / NA 50F163L (TTC→CTC) yqhDNADPH‑dependent aldehyde reductase YqhD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/25);  total (25/25)

CATTCTGCAAACGGGCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTA  >  NC_000913/3155708‑3155962
                                                                                                                                     |                                                                                                                         
caTTCTGCAAACGGGCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCAtt                                                                                                                     <  2:368685/140‑1 (MQ=255)
caTTCTGCAAACGGGCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGAGCTCCAtt                                                                                                                     >  2:884895/1‑140 (MQ=255)
    cTGCAAACGGGCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGc                                                                                                                 <  1:159823/140‑1 (MQ=255)
     tGCAAACGGGTGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGcc                                                                                                                >  2:168106/1‑140 (MQ=255)
          aCGGGCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGt                                                                                                           >  2:546808/1‑140 (MQ=255)
            gggCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTc                                                                                                         >  2:605724/1‑140 (MQ=255)
               cGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGc                                                                                                      <  2:1127064/140‑1 (MQ=255)
               cGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGCAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGc                                                                                                      >  2:646950/1‑140 (MQ=255)
                        gaTTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTg                                                                                             >  2:138056/1‑140 (MQ=255)
                               aGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCt                                                                                      <  1:138056/140‑1 (MQ=255)
                                  gcCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGa                                                                                   >  2:910822/1‑140 (MQ=255)
                                   ccATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGAt                                                                                  >  2:914787/1‑140 (MQ=255)
                                    cATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATc                                                                                 <  1:513281/140‑1 (MQ=255)
                                    cATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATc                                                                                 <  1:919358/140‑1 (MQ=255)
                                    cATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATc                                                                                 <  1:168106/140‑1 (MQ=255)
                                         cGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGAGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGtt                                                                            <  1:884895/140‑1 (MQ=255)
                                                 tgtgtgCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTa                                                                    >  2:735860/1‑140 (MQ=255)
                                                  gtgtgCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTac                                                                   >  2:84411/1‑140 (MQ=255)
                                                           cGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTgccg                                                          <  1:84411/140‑1 (MQ=255)
                                                             cTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTgccgcc                                                        >  2:123809/1‑140 (MQ=255)
                                                               gCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCcgccgc                                                      >  2:91310/1‑140 (MQ=255)
                                                               gCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCcgccgc                                                      <  1:735860/140‑1 (MQ=255)
                                                                  aGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTc                                                   <  1:123809/140‑1 (MQ=255)
                                                                      aCCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGt                                               >  2:215299/1‑140 (MQ=255)
                                                                          gTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCt                                           >  2:584318/1‑140 (MQ=255)
                                                                            tCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTaa                                         <  1:296956/140‑1 (MQ=255)
                                                                                     aaCGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGt                                >  1:921516/1‑140 (MQ=255)
                                                                                     aaCGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGt                                >  1:59659/1‑140 (MQ=255)
                                                                                     aaCGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGt                                >  2:334054/1‑140 (MQ=255)
                                                                                     aaCGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGt                                >  1:205781/1‑140 (MQ=255)
                                                                                     aaCGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGATGGCTAACGGCGTAGt                                >  2:1019191/1‑140 (MQ=255)
                                                                                       cGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTgg                              >  1:623064/1‑140 (MQ=255)
                                                                                          aGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACg                           <  2:623064/140‑1 (MQ=255)
                                                                                          aGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACg                           <  1:646950/140‑1 (MQ=255)
                                                                                          aGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACg                           <  2:205781/140‑1 (MQ=255)
                                                                                          aGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCATGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACg                           <  1:675209/140‑1 (MQ=255)
                                                                                            gcgcGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGcc                         >  2:1113291/1‑140 (MQ=255)
                                                                                            gcgcGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGcc                         <  1:910822/140‑1 (MQ=255)
                                                                                            gcgcGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGcc                         <  1:893057/140‑1 (MQ=255)
                                                                                                  tGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTa                   <  2:79380/140‑1 (MQ=255)
                                                                                                    aTCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTaca                 <  1:584318/140‑1 (MQ=255)
                                                                                                         ccGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCg            <  1:605724/140‑1 (MQ=255)
                                                                                                           gTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTg          >  1:1095496/1‑140 (MQ=255)
                                                                                                            tAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTgg         >  2:624928/1‑140 (MQ=255)
                                                                                                              aaaCCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGaa       <  2:1095496/140‑1 (MQ=255)
                                                                                                               aaCCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAAc      <  2:930429/140‑1 (MQ=255)
                                                                                                                aCCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACa     >  1:375805/1‑140 (MQ=255)
                                                                                                                aCCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACa     <  1:215299/140‑1 (MQ=255)
                                                                                                                   acaGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTa  <  2:375805/140‑1 (MQ=255)
                                                                                                                   acaGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTa  >  2:976114/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                         
CATTCTGCAAACGGGCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTA  >  NC_000913/3155708‑3155962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑