Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,201,912	G→A	G8S (GGT→AGT)	cca →	fused tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase and phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,201,912	0	G	A	100.0%	180.6 / NA	52	G8S (GGT→AGT)	cca	fused tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase and phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/18);  total (34/18)

CCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCA  >  NC_000913/3201780‑3202048                                                                                                                                     |                                                                                                                                         ccGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGt                                                                                                                                   >  2:485252/1‑140 (MQ=255) ccGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGt                                                                                                                                   >  2:662657/1‑140 (MQ=255)         gATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATg                                                                                                                           <  2:680958/140‑1 (MQ=255)           tCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCa                                                                                                                         <  1:550113/140‑1 (MQ=255)                    gcaaacgcaaaGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTTTCTGGTCGGTAGTGCTGTTCGGGATGc                                                                                                                          <  2:535559/130‑1 (MQ=255)                    gcaaacgcaaaGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGc                                                                                                                          >  1:535559/1‑130 (MQ=255)                    gcaaacgcaaaGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAggg                                                                                                                <  1:605464/140‑1 (MQ=255)                     caaacgcaaaGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCggg                                                                                                                              <  2:82501/125‑1 (MQ=255)                     caaacgcaaaGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCggg                                                                                                                              >  1:82501/1‑125 (MQ=255)                              aGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCa                                                                                                      <  2:707031/140‑1 (MQ=255)                              aGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCa                                                                                                      <  1:662657/140‑1 (MQ=255)                                    cTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACa                                                                                                >  2:305952/1‑140 (MQ=255)                                                 aTCGCCTTCTTCGTTGCACTGTCTTATTATTGGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGggtggt                                                                                   >  1:241315/1‑140 (MQ=255)                                                 aTCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGAATTTGACGAAGGGATGATGGCGTGAAGATTTGTCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGggtggt                                                                                   >  2:699618/1‑140 (MQ=255)                                                            cGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTa                                                                        <  1:563902/140‑1 (MQ=255)                                                               tGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGc                                                                     >  2:1012490/1‑140 (MQ=255)                                                                      tCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGga                                                              >  2:253484/1‑140 (MQ=255)                                                                      tCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGga                                                              <  2:970841/140‑1 (MQ=255)                                                                      tCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGga                                                              >  1:562588/1‑140 (MQ=255)                                                                      tCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGga                                                              >  2:867390/1‑140 (MQ=255)                                                                      tCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGAAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGga                                                              >  2:1014427/1‑140 (MQ=255)                                                                                aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  2:480998/1‑140 (MQ=255)                                                                                aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  1:786439/1‑140 (MQ=255)                                                                                aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  1:258287/1‑140 (MQ=255)                                                                                aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  1:143136/1‑140 (MQ=255)                                                                                aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  2:45117/1‑140 (MQ=255)                                                                                aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  2:446829/1‑140 (MQ=255)                                                                                aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  2:244575/1‑140 (MQ=255)                                                                                 ggAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAcg                                                   <  1:305952/140‑1 (MQ=255)                                                                                    aTTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCgg                                                >  1:314320/1‑140 (MQ=255)                                                                                      tgtgTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGc                                              >  2:452425/1‑140 (MQ=255)                                                                                      tgtgTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGc                                              >  1:1124203/1‑140 (MQ=255)                                                                                      tgtgTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGc                                              <  1:1012490/140‑1 (MQ=255)                                                                                      tgtgTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGc                                              <  1:867390/140‑1 (MQ=255)                                                                                       gtgtATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCt                                             <  2:385818/140‑1 (MQ=255)                                                                                        tgtATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTa                                            >  1:646484/1‑140 (MQ=255)                                                                                        tgtATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTa                                            >  1:239138/1‑140 (MQ=255)                                                                                           aTTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACca                                         <  2:241315/140‑1 (MQ=255)                                                                                             ttGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACcagc                                       >  2:445620/1‑140 (MQ=255)                                                                                             ttGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACcagc                                       >  2:111064/1‑140 (MQ=255)                                                                                                aCGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAgg                                    >  2:520083/1‑140 (MQ=255)                                                                                                 cGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGt                                   <  1:485252/140‑1 (MQ=255)                                                                                                    aggaatgatgGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAgg                                >  2:1062288/5‑140 (MQ=255)                                                                                                    aGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAgg                                >  2:1069460/1‑140 (MQ=255)                                                                                                    aGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAgg                                >  1:133811/1‑140 (MQ=255)                                                                                                         tgatgGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCcgcg                           <  2:562588/140‑1 (MQ=255)                                                                                                              gCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGAtttt                      >  2:313080/1‑140 (MQ=255)                                                                                                               cGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTc                     >  2:559261/1‑140 (MQ=255)                                                                                                                 tGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCt                   <  1:253484/140‑1 (MQ=255)                                                                                                                 tGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCt                   >  1:124846/1‑140 (MQ=255)                                                                                                                  gAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCtg                  >  1:475217/1‑140 (MQ=255)                                                                                                                                tCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCg    <  2:314320/140‑1 (MQ=255)                                                                                                                                  ggtagtGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCa  <  1:480998/140‑1 (MQ=255)                                                                                                                                  ggtagtGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCa  <  1:699618/140‑1 (MQ=255)                                                                                                                                  ggtagtGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCa  <  2:258287/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCA  >  NC_000913/3201780‑3202048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑