Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,208,223	T→C	I67T (ATA→ACA)	ttdT →	L‑tartrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,208,223	0	T	C	100.0%	170.3 / NA	46	I67T (ATA→ACA)	ttdT	L‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/17);  total (29/17)

CTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTT  >  NC_000913/3208087‑3208358                                                                                                                                         |                                                                                                                                        ctACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACAct                                                                                                                                      <  2:161284/140‑1 (MQ=255)  tACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACActc                                                                                                                                     <  1:163388/140‑1 (MQ=255)      tCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACtctctc                                                                                                                                 <  2:27601/140‑1 (MQ=255)         ggTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCt                                                                                                                              <  1:22975/140‑1 (MQ=255)            tCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGgc                                                                                                                           >  2:1021083/1‑140 (MQ=255)                   gggCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGtt                                                                                                                    <  2:588410/140‑1 (MQ=255)                   gggCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGtt                                                                                                                    <  1:475944/140‑1 (MQ=255)                   gggCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTACTGtt                                                                                                                    <  2:408355/140‑1 (MQ=255)                        ggAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGcc                                                                                                               >  1:109094/1‑140 (MQ=255)                         gagaACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGccc                                                                                                              >  1:672028/1‑140 (MQ=255)                                aTACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGagcag                                                                                                       <  1:261414/140‑1 (MQ=255)                                        ctctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCa                                                                                               >  1:720057/1‑140 (MQ=255)                                           tACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGcc                                                                                            <  1:1021083/140‑1 (MQ=255)                                           tACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGcc                                                                                            <  2:315630/140‑1 (MQ=255)                                             cTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAg                                                                                          <  2:109094/140‑1 (MQ=255)                                               ttGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGc                                                                                        <  2:720057/140‑1 (MQ=255)                                                 gCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGc                                                                                             <  1:67923/133‑1 (MQ=255)                                                 gCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGc                                                                                             >  2:67923/1‑133 (MQ=255)                                                 gCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCtt                                                                                      >  2:678720/1‑140 (MQ=255)                                                       tttACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAAtt                                                                                >  2:959919/1‑140 (MQ=255)                                                          aCTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCAc                                                                             >  1:610518/1‑140 (MQ=255)                                                               cGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCaa                                                                        <  1:678720/140‑1 (MQ=255)                                                                 tGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCaaaa                                                                      >  1:1127/1‑140 (MQ=255)                                                                   aTCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATc                                                                    >  2:402069/1‑140 (MQ=255)                                                                        tGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCctct                                                               >  2:1127138/1‑140 (MQ=255)                                                                               aTCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGc                                                        >  1:101470/1‑140 (MQ=255)                                                                               aTCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGc                                                        >  2:724879/1‑140 (MQ=255)                                                                               aTCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGc                                                        >  2:383311/1‑140 (MQ=255)                                                                                            tGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGtttt                                           >  1:814381/1‑140 (MQ=255)                                                                                                     ccGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCg                                  >  2:515867/1‑140 (MQ=255)                                                                                                      cGTGGTGGCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCgg                                 >  2:118111/1‑140 (MQ=255)                                                                                                             gCGATGGTGGGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTg                          >  2:552437/1‑140 (MQ=255)                                                                                                                  ggtggGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGa                     >  1:1025519/1‑140 (MQ=255)                                                                                                                    tggGTATATCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGAtt                   >  2:419025/1‑140 (MQ=255)                                                                                                                         atatCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTcgc              <  2:101470/140‑1 (MQ=255)                                                                                                                         atatCCATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTcgc              >  2:134096/1‑140 (MQ=255)                                                                                                                             ccATCATCGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCgccgcc          >  1:197949/1‑140 (MQ=255)                                                                                                                                tcatcGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCttt       >  2:682332/1‑140 (MQ=255)                                                                                                                                tcatcGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGA‑TTTCGCCGCCTTTa      >  2:855003/1‑140 (MQ=255)                                                                                                                                 catcGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTa      >  2:555011/1‑140 (MQ=255)                                                                                                                                  atcGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTAt     >  2:16383/1‑140 (MQ=255)                                                                                                                                  atcGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTAt     >  2:1054296/1‑140 (MQ=255)                                                                                                                                  atcGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTAt     >  2:146759/1‑140 (MQ=255)                                                                                                                                   tcGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATg    <  1:383311/140‑1 (MQ=255)                                                                                                                                    cGCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGt   <  2:627126/140‑1 (MQ=255)                                                                                                                                     gCCACACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGtt  >  2:454624/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTT  >  NC_000913/3208087‑3208358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑