Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,225,837	A→G	H39R (CAC→CGC)	ebgC →	DUF386 domain‑containing evolved beta‑D‑galactosidase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,225,837	0	A	G	100.0%	354.6 / NA	93	H39R (CAC→CGC)	ebgC	DUF386 domain‑containing evolved beta‑D‑galactosidase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (50/43);  total (50/43)

ATTTGCACAGCGAGAATAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCACTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAG  >  NC_000913/3225700‑3225973                                                                                                                                          |                                                                                                                                           aTTTGCACAGCGAGAATAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCt                                                                                                                                          >  1:377417/1‑140 (MQ=255)     gCACAGCGAGAATAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCAt                                                                                                                                      <  2:525450/140‑1 (MQ=255)           cGAGAATAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAACCTGGCGTCGCCCGCTCCATCGGTGa                                                                                                                                <  2:383518/140‑1 (MQ=255)             agaATAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACt                                                                                                                              <  1:705852/140‑1 (MQ=255)               aaTAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCa                                                                                                                            <  2:141752/140‑1 (MQ=255)                   aGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGa                                                                                                                        >  2:646339/1‑140 (MQ=255)                        tGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTAc                                                                                                                   >  1:1086688/1‑140 (MQ=255)                        tGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTAc                                                                                                                   >  2:202744/1‑140 (MQ=255)                         gAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTAcc                                                                                                                  >  1:208976/1‑140 (MQ=255)                          aGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACcg                                                                                                                 >  2:348546/1‑140 (MQ=255)                            gATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAACTATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACcgcg                                                                                                               <  2:784326/140‑1 (MQ=255)                            gATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACcgcg                                                                                                               >  2:760197/1‑140 (MQ=255)                            gATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACcgcg                                                                                                               >  1:784326/1‑140 (MQ=255)                                atcGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACtt                                                                                                                     <  1:338703/130‑1 (MQ=255)                                atcGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACtt                                                                                                                     >  2:338703/1‑130 (MQ=255)                                atcGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑ga                                                                                                         >  2:729345/1‑140 (MQ=255)                                    aTAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑Gagaca                                                                                                     >  2:709610/1‑140 (MQ=255)                                    aTAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑Gagaca                                                                                                     <  1:729345/140‑1 (MQ=255)                                        cTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACt                                                                                                 >  2:222220/1‑140 (MQ=255)                                                 gTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCg                                                                                        <  2:395131/140‑1 (MQ=255)                                                  ttCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGa                                                                                       >  1:698226/1‑140 (MQ=255)                                                  ttCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGa                                                                                       >  1:910256/1‑140 (MQ=255)                                                  ttCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGa                                                                                       >  1:411170/1‑140 (MQ=255)                                                  ttCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGa                                                                                       >  1:314827/1‑140 (MQ=255)                                                  ttCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGa                                                                                       >  1:1030464/1‑140 (MQ=255)                                                   tCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGAt                                                                                      >  2:357633/1‑140 (MQ=255)                                                   tCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGAt                                                                                      >  2:361172/1‑140 (MQ=255)                                                   tCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGAt                                                                                      >  2:270200/1‑140 (MQ=255)                                                   tCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGAt                                                                                      <  2:148323/140‑1 (MQ=255)                                                    ccGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATg                                                                                     <  1:646339/140‑1 (MQ=255)                                                    ccGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATg                                                                                     <  2:411170/140‑1 (MQ=255)                                                    ccGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATg                                                                                     <  2:1090881/140‑1 (MQ=255)                                                     cGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATgc                                                                                    <  2:454539/140‑1 (MQ=255)                                                     cGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATgc                                                                                    <  2:910256/140‑1 (MQ=255)                                                      gCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATgcg                                                                                   <  2:377417/140‑1 (MQ=255)                                                      gCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATgcg                                                                                   <  1:202744/140‑1 (MQ=255)                                                          gATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTAt                                                                               >  2:977471/1‑140 (MQ=255)                                                             ttACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTa                                                                            <  1:760197/140‑1 (MQ=255)                                                                     ctGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAGAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGGG‑‑GAGACAGACTCCCCGACCGATGCGCTATTTACCAGGCAt                                                                    >  2:1004372/1‑140 (MQ=255)                                                                     ctGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCAt                                                                    >  1:358286/1‑140 (MQ=255)                                                                     ctGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCAt                                                                    >  1:37802/1‑140 (MQ=255)                                                                      tGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATc                                                                   >  2:472526/1‑140 (MQ=255)                                                                          aagaagTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACg                                                               <  2:314827/140‑1 (MQ=255)                                                                          aagaagTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACg                                                               <  1:709610/140‑1 (MQ=255)                                                                          aagaagTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACg                                                               <  1:415596/140‑1 (MQ=255)                                                                          aagaagTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACg                                                               <  2:1086688/140‑1 (MQ=255)                                                                          aagaagTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACg                                                               <  1:856184/140‑1 (MQ=255)                                                                               gTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTAtt                                                          <  1:348546/140‑1 (MQ=255)                                                                                  gCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTg                                                       >  1:856123/1‑140 (MQ=255)                                                                                  gCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTg                                                       >  1:574001/1‑140 (MQ=255)                                                                                  gCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTg                                                       <  1:363284/140‑1 (MQ=255)                                                                                  gCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTg                                                       >  1:97043/1‑140 (MQ=255)                                                                                  gCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTGACCGGGCATCGACGCTATTTTg                                                       >  1:803888/1‑140 (MQ=255)                                                                                    aaCGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGaa                                                     <  2:856123/140‑1 (MQ=255)                                                                                    aaCGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGaa                                                     <  1:357633/140‑1 (MQ=255)                                                                                    aaCGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGaa                                                     <  2:97043/140‑1 (MQ=255)                                                                                     aCGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAg                                                    <  2:698226/140‑1 (MQ=255)                                                                                     aCGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAg                                                    <  2:226070/140‑1 (MQ=255)                                                                                       gCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTg                                                  >  2:781190/1‑140 (MQ=255)                                                                                       gCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTg                                                  >  1:432211/1‑140 (MQ=255)                                                                                       gCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTg                                                  <  2:803888/140‑1 (MQ=255)                                                                                         tGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCa                                                <  1:388921/140‑1 (MQ=255)                                                                                         tGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCa                                                >  2:879863/1‑140 (MQ=255)                                                                                         tGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCa                                                >  2:52406/1‑140 (MQ=255)                                                                                           cGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTGGAGAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCa                                                >  2:18044/1‑140 (MQ=255)                                                                                              tGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATtact                                           <  1:222220/140‑1 (MQ=255)                                                                                                    gATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGc                                     <  2:37802/140‑1 (MQ=255)                                                                                                     aTTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCa                                    >  2:30263/1‑140 (MQ=255)                                                                                                     aTTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCa                                    >  2:177591/1‑140 (MQ=255)                                                                                                     aTTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCa                                    >  1:883318/1‑140 (MQ=255)                                                                                                       tGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAg                                  >  1:900825/1‑140 (MQ=255)                                                                                                               tCGACAACATTCAGCCTGGCGTCGCTCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaa                          >  2:851585/1‑140 (MQ=255)                                                                                                                cGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaaa                         >  1:368931/1‑140 (MQ=255)                                                                                                                cGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaaa                         >  2:1074645/1‑140 (MQ=255)                                                                                                                 gACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaaaa                        <  1:1004372/140‑1 (MQ=255)                                                                                                                   caacaTTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaaaaaa                      <  2:358286/140‑1 (MQ=255)                                                                                                                      caTTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaaa                         <  1:162469/134‑1 (MQ=255)                                                                                                                      caTTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaaa                         >  2:162469/1‑134 (MQ=255)                                                                                                                        ttCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGaa                 >  1:1051841/1‑140 (MQ=255)                                                                                                                         tCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAACAAAAAATTGAat                >  1:241559/1‑140 (MQ=255)                                                                                                                           aGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAatat              >  2:399710/1‑140 (MQ=255)                                                                                                                                ggCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGcc         >  2:882018/1‑140 (MQ=255)                                                                                                                                 gCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATgcg           <  1:882472/137‑1 (MQ=255)                                                                                                                                 gCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATgcg           >  2:882472/1‑137 (MQ=255)                                                                                                                                 gCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCg        >  1:625173/1‑140 (MQ=255)                                                                                                                                 gCGTCGCCCGCTCCATCGGGGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCg        >  2:523558/1‑140 (MQ=255)                                                                                                                                   gTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGaa      <  2:432211/140‑1 (MQ=255)                                                                                                                                   gTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGaa      <  2:1051841/140‑1 (MQ=255)                                                                                                                                   gTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGaa      >  1:768209/1‑140 (MQ=255)                                                                                                                                     cGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAACAAAAAATTGAATATGCGCCGAAag    <  2:241559/140‑1 (MQ=255)                                                                                                                                       cccGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAagag  <  1:879863/140‑1 (MQ=255)                                                                                                                                       cccGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAagag  <  1:130145/140‑1 (MQ=255)                                                                                                                                       cccGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAagag  <  1:882018/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                           ATTTGCACAGCGAGAATAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCACTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAG  >  NC_000913/3225700‑3225973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑