Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,233,478	T→C	L605P (CTG→CCG)	fadH →	2,4‑dienoyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,233,478	0	T	C	100.0%	175.8 / NA	47	L605P (CTG→CCG)	fadH	2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/34);  total (13/34)

GATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCT  >  NC_000913/3233341‑3233612                                                                                                                                          |                                                                                                                                       gATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGc                                                                                                                                      >  2:267513/1‑140 (MQ=255)   tGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCAt                                                                                                                                    >  1:674716/1‑140 (MQ=255)      tCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCAtgtg                                                                                                                                 >  2:575641/1‑140 (MQ=255)        cAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATgtggt                                                                                                                               >  1:223034/1‑140 (MQ=255)                  ccAGCAAACCAGGACAGGGGATAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGc                                                                                                                     <  2:930067/140‑1 (MQ=255)                   cAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCg                                                                                                                    <  1:275008/140‑1 (MQ=255)                   cAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCg                                                                                                                    <  1:302018/140‑1 (MQ=255)                        aaCCAGGACAGGTGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACg                                                                                                               <  2:139046/140‑1 (MQ=255)                        aaCCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACg                                                                                                               <  1:383156/140‑1 (MQ=255)                        aaCCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACg                                                                                                               <  2:304890/140‑1 (MQ=255)                        aaCCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACg                                                                                                               <  1:841529/140‑1 (MQ=255)                        aaCCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACg                                                                                                               <  1:494921/140‑1 (MQ=255)                          ccAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCa                                                                                                             <  2:695343/140‑1 (MQ=255)                               aCAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTAt                                                                                                        <  1:791830/140‑1 (MQ=255)                                  ggggTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAg                                                                                                     <  2:674716/140‑1 (MQ=255)                                   gggTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACg                                                                                                               <  1:245172/129‑1 (MQ=255)                                   gggTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACg                                                                                                               >  2:245172/1‑129 (MQ=255)                                   gggTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGc                                                                                                    <  1:194883/140‑1 (MQ=255)                                   gggTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGc                                                                                                    >  2:536991/1‑140 (MQ=255)                                      ttAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGt                                                                                                 <  1:575641/140‑1 (MQ=255)                                       tAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTg                                                                                                <  2:793957/140‑1 (MQ=255)                                       tAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTg                                                                                                <  1:1072507/140‑1 (MQ=255)                                        aGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTgg                                                                                               >  1:996131/1‑140 (MQ=255)                                        aGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTgg                                                                                               >  2:190675/1‑140 (MQ=255)                                        aGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTgg                                                                                               <  2:193529/140‑1 (MQ=255)                                         ggCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGa                                                                                              <  1:489989/140‑1 (MQ=255)                                         ggCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGa                                                                                              <  1:425552/140‑1 (MQ=255)                                         ggCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGa                                                                                              <  2:223034/140‑1 (MQ=255)                                         ggCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGa                                                                                              <  1:1208/140‑1 (MQ=255)                                           cAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACa                                                                                            <  1:805456/140‑1 (MQ=255)                                               accaccGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCa                                                                                                   <  1:485165/129‑1 (MQ=255)                                               accaccGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCa                                                                                                   >  2:485165/1‑129 (MQ=255)                                                ccaccGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAAtgtg                                                                                       <  2:996131/140‑1 (MQ=255)                                                ccaccGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAAtgtg                                                                                       <  1:701745/140‑1 (MQ=255)                                                   ccGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGc                                                                                                    >  1:859978/1‑124 (MQ=255)                                                   ccGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGc                                                                                                    <  2:859978/124‑1 (MQ=255)                                                     ggCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGAt                                                                                  <  2:592032/140‑1 (MQ=255)                                                     ggCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTAACAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGAt                                                                                  <  2:568279/140‑1 (MQ=255)                                                            tCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCa                                                                           <  2:841891/140‑1 (MQ=255)                                                                tCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGc                                                                       <  1:928118/140‑1 (MQ=255)                                                                tCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGc                                                                       <  1:190675/140‑1 (MQ=255)                                                                                             aaaTGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCg                                          <  1:267513/140‑1 (MQ=255)                                                                                                                  aTCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACg                     <  1:536991/140‑1 (MQ=255)                                                                                                                   tCAGAAGATTGACGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACgg                    >  1:1008239/1‑140 (MQ=255)                                                                                                                              aCGATGACGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAAtt         >  1:7578/1‑140 (MQ=255)                                                                                                                                     cGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCt  >  1:888215/1‑140 (MQ=255)                                                                                                                                     cGGGCCGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCt  <  2:7578/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       GATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCT  >  NC_000913/3233341‑3233612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑