Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	425,133	T→C	G41G (GGT→GGC)	queA →	tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	425,133	0	T	C	100.0%	270.9 / NA	72	G41G (GGT→GGC)	queA	tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/37);  total (35/37)

TCTGAGTCAGTGTCATGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGTACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTC  >  NC_000913/424997‑425268                                                                                                                                         |                                                                                                                                        tCTGAGTCAGTGTCATGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACt                                                                                                                                      >  1:376201/1‑140 (MQ=255)     aGTCAGTGTCATGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCa                                                                                                                                  >  1:390620/1‑140 (MQ=255)      gTCAGTGTCATGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCAc                                                                                                                                 <  2:71041/140‑1 (MQ=255)         aGTGTCATGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGa                                                                                                                              <  1:588714/140‑1 (MQ=255)               aTGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACt                                                                                                                        >  1:734275/1‑140 (MQ=255)               aTGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACt                                                                                                                        >  2:399436/1‑140 (MQ=255)                tGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTa                                                                                                                          >  1:1057984/1‑137 (MQ=255)                tGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTa                                                                                                                          <  2:1057984/137‑1 (MQ=255)                tGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACtt                                                                                                                       >  1:468155/1‑140 (MQ=255)                tGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACtt                                                                                                                       >  1:464408/1‑140 (MQ=255)                 gcgcGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTAc                                                                                                                         >  2:892854/1‑137 (MQ=255)                 gcgcGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTAc                                                                                                                         <  1:892854/137‑1 (MQ=255)                   gcgTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGAt                                                                                                                    <  1:1003744/140‑1 (MQ=255)                   gcgTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGAt                                                                                                                    <  2:468155/140‑1 (MQ=255)                   gcgTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGAt                                                                                                                    <  1:160654/140‑1 (MQ=255)                   gcgTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGAt                                                                                                                    <  2:734275/140‑1 (MQ=255)                    cgTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATa                                                                                                                   >  1:256316/1‑140 (MQ=255)                      ttACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAg                                                                                                                 >  2:226477/1‑140 (MQ=255)                       tACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGc                                                                                                                <  2:138006/140‑1 (MQ=255)                       tACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGc                                                                                                                >  1:668472/1‑140 (MQ=255)                        aCCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCt                                                                                                               <  1:302281/140‑1 (MQ=255)                              ttCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAAccc                                                                                                         >  1:220711/1‑140 (MQ=255)                              ttCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAAccc                                                                                                         >  1:524801/1‑140 (MQ=255)                              ttCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAAccc                                                                                                         >  1:695501/1‑140 (MQ=255)                                  ccTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGc                                                                                                     <  2:695501/140‑1 (MQ=255)                                  ccTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGc                                                                                                     <  2:220711/140‑1 (MQ=255)                                      tGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGAtc                                                                                                 >  1:814442/1‑140 (MQ=255)                                        aaTTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGAtctt                                                                                               <  2:314310/140‑1 (MQ=255)                                        aaTTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGAtctt                                                                                               >  1:146866/1‑140 (MQ=255)                                        aaTTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGAtctt                                                                                               >  2:872274/1‑140 (MQ=255)                                         aTTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGAtcttc                                                                                              <  1:532797/140‑1 (MQ=255)                                           tGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTg                                                                                            <  2:668472/140‑1 (MQ=255)                                              ccGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGtt                                                                                         <  1:515201/140‑1 (MQ=255)                                              ccGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGtt                                                                                         <  2:524801/140‑1 (MQ=255)                                               cGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGttt                                                                                        <  1:226477/140‑1 (MQ=255)                                               cGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGttt                                                                                        <  2:1114379/140‑1 (MQ=255)                                                       tGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTaataat                                                                                <  1:526458/140‑1 (MQ=255)                                                            gCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCcg                                                                           >  1:401593/1‑140 (MQ=255)                                                              ccACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATGCCcgcg                                                                         <  2:350404/140‑1 (MQ=255)                                                                       cATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCgg                                                                >  1:102520/1‑140 (MQ=255)                                                                        aTGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGgc                                                               <  2:256316/140‑1 (MQ=255)                                                                         tGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGgcg                                                              >  2:436290/1‑140 (MQ=255)                                                                         tGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGgcg                                                              >  1:613432/1‑140 (MQ=255)                                                                           ccTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATAcc                                                                             >  2:1031968/1‑123 (MQ=255)                                                                           ccTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATAcc                                                                             <  1:1031968/123‑1 (MQ=255)                                                                            cTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCcgcg                                                                         <  1:92857/126‑1 (MQ=255)                                                                            cTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCcgcg                                                                         >  2:92857/1‑126 (MQ=255)                                                                            cTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGgcgcgc                                                           >  2:152393/1‑140 (MQ=255)                                                                            cTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGgcgcgc                                                           <  2:613432/140‑1 (MQ=255)                                                                            cTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGgcaacg                                                           >  1:428043/1‑136 (MQ=255)                                                                             tGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGcc                                                          <  2:401593/140‑1 (MQ=255)                                                                              gAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGgcgcgc                                                           >  2:938547/1‑138 (MQ=255)                                                                              gAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGgcgcgc                                                           <  1:938547/138‑1 (MQ=255)                                                                              gAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGgcaacgtg                                                         <  2:428043/140‑7 (MQ=255)                                                                               aaCGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTg                                                        >  2:100210/1‑140 (MQ=255)                                                                                   cAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTg                                                    <  1:399436/140‑1 (MQ=255)                                                                                     gTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTggg                                                  >  1:154623/1‑140 (MQ=255)                                                                                              gTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGcc                                         >  1:307055/1‑140 (MQ=255)                                                                                                      tCGCTGGACGGGCCGACGGGCGCGCTGACGCATGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGcggcgg                                 >  2:850400/1‑140 (MQ=255)                                                                                                      tCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATCCCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGcggcgg                                 <  2:845168/140‑1 (MQ=255)                                                                                                      tCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGcggcgg                                 <  1:100210/140‑1 (MQ=255)                                                                                                      tCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGcggcgg                                 <  2:146866/140‑1 (MQ=255)                                                                                                      tCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGcggcgg                                 <  1:152393/140‑1 (MQ=255)                                                                                                       cGCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCggcggc                                <  1:559706/140‑1 (MQ=255)                                                                                                        gCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTTAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCa                               >  1:997328/1‑140 (MQ=255)                                                                                                        gCTGGACGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCa                               >  1:140524/1‑140 (MQ=255)                                                                                                              cGGGCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTg                         <  2:390620/140‑1 (MQ=255)                                                                                                                ggCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGaa                       >  1:611744/1‑140 (MQ=255)                                                                                                                 gCCGACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAg                      >  1:1127152/1‑140 (MQ=255)                                                                                                                    gACGGGCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGtcc                   <  2:307055/140‑3 (MQ=255)                                                                                                                        ggCGCGCTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGt               <  2:376201/140‑1 (MQ=255)                                                                                                                           gcgcTGACGCACGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGa            >  1:900526/1‑140 (MQ=255)                                                                                                                                     aCGGCACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTc  <  2:611744/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCTGAGTCAGTGTCATGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGTACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTC  >  NC_000913/424997‑425268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑