Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,299,839	T→C	E26G (GAA→GGA)	yhbP ←	putative FMN binding protein YhbP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,299,839	0	T	C	100.0%	223.6 / NA	60	E26G (GAA→GGA)	yhbP	putative FMN binding protein YhbP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/37);  total (23/37)

GCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGA  >  NC_000913/3299705‑3299969                                                                                                                                       |                                                                                                                                   gCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCt                                                                                                                               <  2:783599/140‑1 (MQ=255) gCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCt                                                                                                                               <  1:894255/140‑1 (MQ=255)  cTGACCGTTTACCGTTCCGGCAACCGCAGCCCGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCtt                                                                                                                              >  1:298102/1‑140 (MQ=255)     aCCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCct                                                                                                                           >  1:688182/1‑140 (MQ=255)      ccGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCctg                                                                                                                          <  1:805930/140‑1 (MQ=255)      ccGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCctg                                                                                                                          <  1:651830/140‑1 (MQ=255)      ccGTTTACCGTTCCGGCAACCGCAGCCCGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCctg                                                                                                                          <  2:298102/140‑1 (MQ=255)               gTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGcacaca                                                                                                                 >  2:949339/1‑140 (MQ=255)               gTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGcacaca                                                                                                                 <  1:137307/140‑1 (MQ=255)               gTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGcacaca                                                                                                                 >  1:344278/1‑140 (MQ=255)                ttCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCctg                                                                                                                          <  2:184259/130‑1 (MQ=255)                ttCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCctg                                                                                                                          >  1:184259/1‑130 (MQ=255)                ttCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCacacac                                                                                                                >  2:543403/1‑140 (MQ=255)                   cGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCaa                                                                                                             >  1:214100/1‑140 (MQ=255)                    ggCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAg                                                                                                            <  1:543403/140‑1 (MQ=255)                    ggCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAg                                                                                                            <  1:949339/140‑1 (MQ=255)                       aaCCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCctg                                                                                                                          >  1:614293/1‑123 (MQ=255)                       aaCCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCctg                                                                                                                          <  2:614293/123‑1 (MQ=255)                       aaCCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGa                                                                                                         <  1:753535/140‑1 (MQ=255)                         ccGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGaca                                                                                                       <  1:53982/140‑1 (MQ=255)                         ccGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGaca                                                                                                       <  2:944928/140‑1 (MQ=255)                                       ccACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTgc                                                                                         <  2:214100/140‑1 (MQ=255)                                         aCTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTgcca                                                                                       >  2:452280/1‑140 (MQ=255)                                         aCTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTgcca                                                                                       <  2:688182/140‑1 (MQ=255)                                         aCTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTgcca                                                                                       >  1:914269/1‑140 (MQ=255)                                          cTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTgccag                                                                                      >  2:620795/1‑140 (MQ=255)                                             aTCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTgccagcca                                                                                   <  1:270240/140‑1 (MQ=255)                                              tCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCaa                                                                                  >  2:1029465/1‑140 (MQ=255)                                              tCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCaa                                                                                  <  2:502923/140‑1 (MQ=255)                                                tGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCTAGTGACAACATGTTGTTTTGCCAGCCAACg                                                                                >  2:694335/1‑140 (MQ=255)                                                  cgcgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGc                                                                              <  1:865160/140‑1 (MQ=255)                                                  cgcgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGc                                                                              >  1:1012101/1‑140 (MQ=255)                                                    cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTgcca                                                                                       <  1:384627/129‑1 (MQ=255)                                                    cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTgcca                                                                                       >  2:384627/1‑129 (MQ=255)                                                             ttttttCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATg                                                                   >  1:903778/1‑140 (MQ=255)                                                                        gTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCAt                                                        >  2:877095/1‑140 (MQ=255)                                                                             aaTGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTg                                                   <  1:460538/140‑1 (MQ=255)                                                                               tGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGtt                                                 <  1:694335/140‑1 (MQ=255)                                                                               tGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGtt                                                 <  1:398685/140‑1 (MQ=255)                                                                               tGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGtt                                                 <  1:40998/140‑1 (MQ=255)                                                                               tGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGtt                                                 <  1:620795/140‑1 (MQ=255)                                                                               tGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGtt                                                 <  2:914269/140‑1 (MQ=255)                                                                               tGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGtt                                                 <  2:193595/140‑1 (MQ=255)                                                                               tGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGtt                                                 <  1:1029465/140‑1 (MQ=255)                                                                               tGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGtt                                                 <  1:1128762/140‑1 (MQ=255)                                                                                       gCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTta                                         >  2:256372/1‑140 (MQ=255)                                                                                         gACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTtct                                               >  1:699662/1‑132 (MQ=255)                                                                                         gACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCCGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTtct                                               <  2:699662/132‑1 (MQ=255)                                                                                              tCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACtgtg                                  >  1:663992/1‑140 (MQ=255)                                                                                                 gcgcATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGc                               <  1:877095/140‑1 (MQ=255)                                                                                                 gcgcATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGc                               <  2:663992/140‑1 (MQ=255)                                                                                                  cgcATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGcc                              <  2:1113231/140‑1 (MQ=255)                                                                                                       cAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACCTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGAcc                         <  2:903778/140‑1 (MQ=255)                                                                                                       cAAAAAGATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGAcc                         >  2:838055/1‑140 (MQ=255)                                                                                                             gATAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACAAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGAGGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACa                   <  2:152448/140‑1 (MQ=255)                                                                                                               tAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATg                 <  1:426937/140‑1 (MQ=255)                                                                                                               tAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATg                 >  2:247988/1‑140 (MQ=255)                                                                                                               tAAAAGGCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATg                 >  2:1000757/1‑140 (MQ=255)                                                                                                                    ggCATTAGCGCACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACg            <  1:1098804/140‑1 (MQ=255)                                                                                                                              cACCAGAGTCCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGa  <  1:425279/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   GCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATCAAAAAGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCCTGCTGCACACACCAAGTGACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGA  >  NC_000913/3299705‑3299969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑