Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,404,262	T→C	V260A (GTC→GCC)	yhdH →	acrylyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,404,262	0	T	C	100.0%	147.9 / NA	41	V260A (GTC→GCC)	yhdH	acrylyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/23);  total (18/23)

TGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCAAAAGAGATATCTCTGTCAGAGGCA  >  NC_000913/3404131‑3404392                                                                                                                                    |                                                                                                                                   tGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGc                                                                                                                            <  2:1047986/140‑1 (MQ=255)  gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCa                                                                                                                           <  1:1022887/140‑1 (MQ=255)    cacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCCCGGGCATGCCATTTTTTTTGCGTAATGCCCGGTTTCaaa                                                                                                                         >  2:936433/1‑139 (MQ=255)      ccGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAggg                                                                                                                       <  2:16556/140‑1 (MQ=255)         ttGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTg                                                                                                                    <  2:455657/140‑1 (MQ=255)          tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTgg                                                                                                                   <  1:1097172/140‑1 (MQ=255)          tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTgg                                                                                                                   <  1:314646/140‑1 (MQ=255)          tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTACTCTGCCAACCACGGTCATGCCATTTATTTTGCGTAATGCCCGTTTGCAAGGGGTgg                                                                                                                   >  1:814952/1‑140 (MQ=255)           ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGa                                                                                                                  >  1:891392/1‑140 (MQ=255)                 aaaGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGt                                                                                                            <  1:896730/140‑1 (MQ=255)                         ggCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGc                                                                                                    <  1:273310/140‑1 (MQ=255)                                           aaTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGcac                                                                                  <  2:289138/140‑1 (MQ=255)                                               aaTTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGc                                                                              <  1:730670/140‑1 (MQ=255)                                               aaTTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGc                                                                              <  2:287527/140‑1 (MQ=255)                                                      gcggcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCAAACCCCGGCGTTCCCTTTTTTTTTCGGAATGCCCCTTTTGAAGGGGGGGGTTCCGTTAATAAGCCCCCCGAAACCCCCGCACAAACCCgggca                                                                       >  2:861414/1‑137 (MQ=255)                                                        ggcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCCACCACGGGCATGCCCTTTTTTTTGCGTAATGCCCGTTTGCAAGGGGGGGGTTCAGTAAAGACGCCCCCAGAACGCCGCGCCCAAGCCCGGGAGCg                                                                     >  2:638391/1‑140 (MQ=255)                                                         gcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGGCATGCCATTTATTTTGCGTAATGCCCGTTTGCAAGGGGGGGGTTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGa                                                                    >  1:662290/1‑140 (MQ=255)                                                          cTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGAc                                                                   <  1:1119518/140‑1 (MQ=255)                                                              gTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGt                                                               >  2:220561/1‑140 (MQ=255)                                                                ggcggcCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCg                                                             <  1:220561/140‑1 (MQ=255)                                                                            tCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCgg                                                 >  2:917843/1‑140 (MQ=255)                                                                              tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa                                               <  1:13635/140‑1 (MQ=255)                                                                                gCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATc                                             <  1:638391/140‑1 (MQ=255)                                                                                gCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATc                                             <  1:861414/140‑1 (MQ=255)                                                                                 cGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCa                                            >  2:406652/1‑140 (MQ=255)                                                                                     tggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCt                                        >  2:210095/1‑140 (MQ=255)                                                                                     tggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGGGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCt                                        >  1:379170/1‑140 (MQ=255)                                                                                     gggtTTTTGTCTCCCAACCACGGGCCTGCCATTTATTCTGCGTAATGCCCGTTTGCCAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCt                                        >  2:1126029/2‑140 (MQ=255)                                                                                          ttACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa                                               >  1:138687/1‑128 (MQ=255)                                                                                          ttACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa                                               <  2:138687/128‑1 (MQ=255)                                                                                          ttACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATAcc                                   >  2:11698/1‑140 (MQ=255)                                                                                            aCTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGCTCGCCGATTTACCGGAATCATTCTATACCCa                                 <  2:891392/140‑1 (MQ=255)                                                                                                  ccAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAggcggc                           >  1:418665/1‑140 (MQ=255)                                                                                                  ccAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAggcggc                           >  2:528389/1‑140 (MQ=255)                                                                                                   cAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCa                          <  1:955017/140‑1 (MQ=255)                                                                                                   cAACCACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCa                          <  1:528389/140‑1 (MQ=255)                                                                                                       cACGGTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGCGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCAAAag                      >  2:720866/1‑140 (MQ=255)                                                                                                          ggTCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCAAAAgaga                   >  2:820427/1‑140 (MQ=255)                                                                                                            tCATGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCAAAAGAGata                 <  2:963298/140‑1 (MQ=255)                                                                                                               tGCCATTTATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCAAAAGAGATAtct              >  2:25254/1‑140 (MQ=255)                                                                                                                      tATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCAAAAGAGATATCTCTGTCag       <  2:662290/140‑1 (MQ=255)                                                                                                                      tATTCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCAAAAGAGATATCTCTGTCag       >  2:140461/1‑140 (MQ=255)                                                                                                                         tCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCAAAAGAGATATCTCTGTCAGAgg    >  2:1024522/1‑140 (MQ=255)                                                                                                                         tCTGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCAAAAGAGATATCTCTGTCAGAgg    >  2:185644/1‑140 (MQ=255)                                                                                                                           tGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCAAAAGAGATATCTCTGTCAGAGGCa  <  1:936433/140‑1 (MQ=255)                                                                                                                           tGCGTAATGCCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCAAAAGAGATATCTCTGTCAGAGGCa  >  1:285739/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                   TGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCGGCAAAAGAGATATCTCTGTCAGAGGCA  >  NC_000913/3404131‑3404392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑