Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,546,408	T→C	intergenic (+209/‑151)	nfuA → / → gntT	iron‑sulfur cluster carrier protein NfuA/high‑affinity gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,546,408	0	T	C	100.0%	302.7 / NA	80	intergenic (+209/‑151)	nfuA/gntT	iron‑sulfur cluster carrier protein NfuA/high‑affinity gluconate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/42);  total (38/42)

AGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAA  >  NC_000913/3546272‑3546544                                                                                                                                         |                                                                                                                                         aGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCAcc                                                                                                                                       <  2:112411/140‑1 (MQ=255)     gTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAAc                                                                                                                                   <  2:152400/140‑1 (MQ=255)      ttGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACa                                                                                                                                  <  2:329591/140‑1 (MQ=255)       tGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCAcc                                                                                                                                       >  1:138082/1‑134 (MQ=255)       tGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCAcc                                                                                                                                       <  2:138082/134‑1 (MQ=255)       tGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTATCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACAt                                                                                                                                 >  1:1119985/1‑140 (MQ=255)        ggCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTTTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATa                                                                                                                                <  1:652528/140‑1 (MQ=255)        ggCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATa                                                                                                                                <  2:390135/140‑1 (MQ=255)          cgcAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACt                                                                                                                              <  2:72262/140‑1 (MQ=255)            cagcaTTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTcc                                                                                                                            <  1:953088/140‑1 (MQ=255)             agcaTTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCt                                                                                                                           <  1:1036496/140‑1 (MQ=255)                  tGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTGCCCGTATCATTCACATGGGCACCAAACATACTCCTGACAt                                                                                                                      <  2:1007117/140‑1 (MQ=255)                       tGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGAc                                                                                                                 >  2:943010/1‑140 (MQ=255)                        gTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACt                                                                                                                <  2:876198/140‑1 (MQ=255)                         tCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTa                                                                                                               >  1:339755/1‑140 (MQ=255)                         tCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTa                                                                                                               <  1:659846/140‑1 (MQ=255)                          cAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTAc                                                                                                              <  2:629618/140‑1 (MQ=255)                           aaaTCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGTCACCAAACATACTCCTGACATCTGACTACa                                                                                                             <  1:216882/140‑1 (MQ=255)                            aaTCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACaa                                                                                                            >  2:683872/1‑140 (MQ=255)                                cTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACaataat                                                                                                        >  2:707212/1‑140 (MQ=255)                                     ttCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGtt                                                                                                   <  1:986452/140‑1 (MQ=255)                                     ttCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGtt                                                                                                   <  1:519855/140‑1 (MQ=255)                                       cGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGtttt                                                                                                 <  2:174658/140‑1 (MQ=255)                                        gTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTa                                                                                                <  1:778666/140‑1 (MQ=255)                                         tttCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAg                                                                                               <  1:868138/140‑1 (MQ=255)                                         tttCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAg                                                                                               >  1:933460/1‑140 (MQ=255)                                              ccGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGt                                                                                          <  2:933460/140‑1 (MQ=255)                                                   gtaataTGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAg                                                                                     >  2:346359/2‑140 (MQ=255)                                                   ataataTGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAg                                                                                     >  1:315922/1‑140 (MQ=255)                                                          gACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCgtggt                                                                              <  1:385629/140‑1 (MQ=255)                                                            ccAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGc                                                                            >  2:599903/1‑140 (MQ=255)                                                            ccAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGc                                                                            <  1:346359/140‑1 (MQ=255)                                                            ccAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTGAGTGGGTATCAGTCGTGGTGc                                                                            >  2:1033956/1‑140 (MQ=255)                                                              aaCCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCgtggt                                                                              >  2:1023843/1‑136 (MQ=255)                                                              aaCCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCgtggt                                                                              <  1:1023843/136‑1 (MQ=255)                                                              aaCCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCg                                                                          >  1:330787/1‑140 (MQ=255)                                                               aCCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGc                                                                         >  1:260389/1‑140 (MQ=255)                                                                ccTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCa                                                                        >  2:1112115/1‑140 (MQ=255)                                                                ccTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCa                                                                        >  1:933417/1‑140 (MQ=255)                                                                ccTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCa                                                                        >  1:404442/1‑140 (MQ=255)                                                                  tctcATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAat                                                                      >  1:1095304/1‑140 (MQ=255)                                                                    tcATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAatat                                                                    >  2:5565/1‑140 (MQ=255)                                                                      aTAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATAtct                                                                  <  1:125564/140‑1 (MQ=255)                                                                           ttaaatttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGtt                                                             >  1:981277/1‑140 (MQ=255)                                                                            taaatttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATTCTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTc                                                            <  2:1080816/140‑1 (MQ=255)                                                                              aatttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAatat                                                                    <  2:660570/130‑1 (MQ=255)                                                                              aatttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAatat                                                                    >  1:660570/1‑130 (MQ=255)                                                                                tttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGa                                                        <  2:330787/140‑1 (MQ=255)                                                                                tttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGa                                                        <  2:723855/140‑1 (MQ=255)                                                                                 ttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGAt                                                       <  2:404442/140‑1 (MQ=255)                                                                                    ccccGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTgg                                                    <  1:388214/140‑1 (MQ=255)                                                                                    ccccGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTgg                                                    <  2:1119985/140‑1 (MQ=255)                                                                                    ccccGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTgg                                                    >  2:388214/1‑140 (MQ=255)                                                                                      ccGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGa                                                  <  2:1095304/140‑1 (MQ=255)                                                                                         ctctGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATaa                                               >  1:624796/1‑140 (MQ=255)                                                                                              gTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTaga                                          >  1:873369/1‑140 (MQ=255)                                                                                                   tctcAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTgg                                                    >  1:592874/1‑125 (MQ=255)                                                                                                   tctcAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTgg                                                    <  2:592874/125‑1 (MQ=255)                                                                                                    ctcAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTgt                                    >  1:1120934/1‑140 (MQ=255)                                                                                                      cAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTgtcg                                  >  1:245514/1‑140 (MQ=255)                                                                                                        aaCGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTgtcgtc                                >  1:1110682/1‑140 (MQ=255)                                                                                                        aaCGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTgtcgtc                                >  1:849740/1‑140 (MQ=255)                                                                                                               gacgTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAAt                         <  2:981277/137‑1 (MQ=255)                                                                                                               gATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAAt                         <  2:849740/140‑1 (MQ=255)                                                                                                                    tACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACg                    <  2:339755/140‑1 (MQ=255)                                                                                                                     aCCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGt                   >  2:85749/1‑140 (MQ=255)                                                                                                                     aCCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGt                   >  2:1011782/1‑140 (MQ=255)                                                                                                                      cccGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGtt                  <  2:58811/140‑1 (MQ=255)                                                                                                                          tATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTAccc              >  1:979735/1‑140 (MQ=255)                                                                                                                          tATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTAccc              >  1:650057/1‑140 (MQ=255)                                                                                                                           aTCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGaaaa                          <  2:941931/127‑1 (MQ=255)                                                                                                                           aTCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGaaaa                          >  1:941931/1‑127 (MQ=255)                                                                                                                            tCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCAt            <  2:933417/140‑1 (MQ=255)                                                                                                                               ttCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGtt                  <  2:783566/131‑1 (MQ=255)                                                                                                                               ttCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGtt                  >  1:783566/1‑131 (MQ=255)                                                                                                                               ttCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAAc         >  1:545357/1‑140 (MQ=255)                                                                                                                               ttCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGACGTCAGAAAATTGACGTTACCCATAAc         <  2:624796/140‑1 (MQ=255)                                                                                                                                    aTGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATg    >  1:72299/1‑140 (MQ=255)                                                                                                                                     tGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGa   <  2:537639/140‑1 (MQ=255)                                                                                                                                      gggCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGaa  >  1:611154/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         AGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAA  >  NC_000913/3546272‑3546544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑