Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,574,808	T→C	E24G (GAG→GGG)	asd ←	aspartate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,574,808	0	T	C	100.0%	251.8 / NA	68	E24G (GAG→GGG)	asd	aspartate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/34);  total (34/34)

TATCGAGGGCCTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTT  >  NC_000913/3574677‑3574943                                                                                                                                    |                                                                                                                                        tatCGAGGGCCTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAAcc                                                                                                                                 >  2:301296/1‑140 (MQ=255)    cGAGGGCCTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATg                                                                                                                              <  1:301296/140‑1 (MQ=255)    cGAGGGCCTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATg                                                                                                                              <  1:224579/140‑1 (MQ=255)        ggCCTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGtt                                                                                                                          >  2:847885/1‑140 (MQ=255)          ccTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGc                                                                                                                        >  1:769226/1‑140 (MQ=255)            tttAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCAt                                                                                                                      <  1:682669/140‑1 (MQ=255)            tttAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCAt                                                                                                                      <  1:847885/140‑1 (MQ=255)                 cgcCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGaa                                                                                                                 <  2:769226/140‑1 (MQ=255)                   ccTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACg                                                                                                               >  1:305282/1‑140 (MQ=255)                    cTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGc                                                                                                                             >  2:899119/1‑125 (MQ=255)                    cTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGc                                                                                                                             <  1:899119/125‑1 (MQ=255)                    cTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACgg                                                                                                              >  2:52780/1‑140 (MQ=255)                     tCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGa                                                                                                             <  2:9239/140‑1 (MQ=255)                     tCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGa                                                                                                             >  1:231204/1‑140 (MQ=255)                     tCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGa                                                                                                             >  1:277531/1‑140 (MQ=255)                              aaGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGACTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCAt                                                                                                    >  2:556488/1‑140 (MQ=255)                                    tCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGa                                                                                                        >  2:941226/1‑130 (MQ=255)                                    tCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGa                                                                                                        <  1:941226/130‑1 (MQ=255)                                    tCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACcgcg                                                                                              >  2:80959/1‑140 (MQ=255)                                     ccTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGc                                                                                                           >  2:510880/1‑126 (MQ=255)                                     ccTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGc                                                                                                           <  1:510880/126‑1 (MQ=255)                                              tgCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATa                                                                                    >  2:747359/1‑140 (MQ=255)                                               gCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACTATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATaa                                                                                   >  2:1128335/1‑140 (MQ=255)                                                   gTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAAcc                                                                               >  1:1040311/1‑140 (MQ=255)                                                       ttCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACa                                                                           <  1:406901/140‑1 (MQ=255)                                                        tCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACAt                                                                          >  1:1050853/1‑140 (MQ=255)                                                             cAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTc                                                                     >  2:893397/1‑140 (MQ=255)                                                                       cgcAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGttt                                                           >  1:93745/1‑140 (MQ=255)                                                                       cgcAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGttt                                                           >  1:537909/1‑140 (MQ=255)                                                                          aGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGtttttt                                                        <  1:1029607/140‑1 (MQ=255)                                                                          aGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGtttttt                                                        <  1:1113315/140‑1 (MQ=255)                                                                          aGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGtttttt                                                        <  2:537909/140‑1 (MQ=255)                                                                                 ccAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCa                                                 >  2:1129349/1‑140 (MQ=255)                                                                                     gCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGa                                             >  1:499145/1‑140 (MQ=255)                                                                                     gCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGa                                             >  1:1002517/1‑140 (MQ=255)                                                                                      cTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAt                                            >  1:968517/1‑140 (MQ=255)                                                                                      cTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAt                                            <  2:372677/140‑1 (MQ=255)                                                                                      cTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAt                                            >  2:10437/1‑140 (MQ=255)                                                                                         agaAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtg                                         <  1:1129349/140‑1 (MQ=255)                                                                                         agaAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtg                                         <  1:893397/140‑1 (MQ=255)                                                                                           aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACTATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  1:1128335/140‑1 (MQ=255)                                                                                           aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  1:728775/140‑1 (MQ=255)                                                                                           aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  1:495565/140‑1 (MQ=255)                                                                                           aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  2:93745/140‑1 (MQ=255)                                                                                           aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  2:305282/140‑1 (MQ=255)                                                                                           aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  1:52780/140‑1 (MQ=255)                                                                                           aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  1:80959/140‑1 (MQ=255)                                                                                           aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  2:1040311/140‑1 (MQ=255)                                                                                                   aagaagACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       >  1:759490/1‑132 (MQ=255)                                                                                                   aagaagACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  2:415764/132‑1 (MQ=255)                                                                                                   aagaagACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  2:759490/132‑1 (MQ=255)                                                                                                   aagaagACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       >  1:415764/1‑132 (MQ=255)                                                                                                     gaagaCAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAAt                             >  2:470190/1‑140 (MQ=255)                                                                                                        gaCAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGGGCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAAtgtg                          <  2:277531/140‑1 (MQ=255)                                                                                                        gaCAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAAtgtg                          >  2:667673/1‑140 (MQ=255)                                                                                                        gaCAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAAtgtg                          >  1:121349/1‑140 (MQ=255)                                                                                                            gggCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCgg                      >  1:646686/1‑140 (MQ=255)                                                                                                             ggCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGt                     >  1:470041/1‑140 (MQ=255)                                                                                                             ggCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGt                     <  2:646686/140‑1 (MQ=255)                                                                                                             ggCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGt                     <  2:121349/140‑1 (MQ=255)                                                                                                             ggCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGt                     >  1:67562/1‑140 (MQ=255)                                                                                                                  aTGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTctcct                <  1:10437/140‑1 (MQ=255)                                                                                                                   tGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTctcctc               <  1:156707/140‑1 (MQ=255)                                                                                                                        tCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGc          <  2:67562/140‑1 (MQ=255)                                                                                                                         cGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGca         <  2:1050853/140‑1 (MQ=255)                                                                                                                         cGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGca         <  1:667673/140‑1 (MQ=255)                                                                                                                           aaGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGcaca       <  2:394441/140‑1 (MQ=255)                                                                                                                                gcgcCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCttt  >  2:748816/1‑140 (MQ=255)                                                                                                                                gcgcCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCttt  >  2:394176/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        TATCGAGGGCCTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTT  >  NC_000913/3574677‑3574943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑