Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,054,887	A→G	V131A (GTT→GCT)	glnG ←	DNA‑binding transcriptional dual regulator NtrC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,054,887	0	A	G	100.0%	369.1 / NA	97	V131A (GTT→GCT)	glnG	DNA‑binding transcriptional dual regulator NtrC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/54);  total (43/54)

CAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGAT  >  NC_000913/4054751‑4055022                                                                                                                                         |                                                                                                                                        cAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCAt                                                                                                                                      >  1:279558/1‑140 (MQ=255)    ttCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTAc                                                                                                                                   <  1:529092/140‑1 (MQ=255)      cTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTAcgc                                                                                                                                 <  2:1055546/140‑1 (MQ=255)      cTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTAcgc                                                                                                                                 >  1:1055546/1‑140 (MQ=255)      cTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTAcgc                                                                                                                                 >  1:652317/1‑140 (MQ=255)       tttACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTAcgcg                                                                                                                                <  1:353326/140‑1 (MQ=255)           ccGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGgctg                                                                                                                            <  1:1086745/140‑1 (MQ=255)              gTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGgctgctg                                                                                                                         >  1:878068/1‑140 (MQ=255)              gTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGgctgctg                                                                                                                         >  1:896978/1‑140 (MQ=255)              gTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGgctgctg                                                                                                                         >  2:73853/1‑140 (MQ=255)              gTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGgc                                                                                                                              >  1:504858/1‑135 (MQ=255)              gTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGgc                                                                                                                              <  2:504858/135‑1 (MQ=255)              gTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAACAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGgctgctg                                                                                                                         >  2:88131/1‑140 (MQ=255)               tGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGgctgctgc                                                                                                                        >  2:321284/1‑140 (MQ=255)                gCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGgctgctgct                                                                                                                       <  2:878068/140‑1 (MQ=255)                gCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGgctgctgct                                                                                                                       >  2:566064/1‑140 (MQ=255)                gCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGgctgctgct                                                                                                                       <  1:145397/140‑1 (MQ=255)                gCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGgctgctgct                                                                                                                       <  1:378381/140‑1 (MQ=255)                gCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGgctgctgct                                                                                                                       <  2:285898/140‑1 (MQ=255)                    gATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATTCGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTc                                                                                                                   <  1:88131/140‑1 (MQ=255)                    gATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTc                                                                                                                   <  1:591819/140‑1 (MQ=255)                      ttCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCt                                                                                                                 >  1:281029/1‑140 (MQ=255)                      ttCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCt                                                                                                                 >  1:370772/1‑140 (MQ=255)                        cGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTgg                                                                                                               <  1:73853/140‑1 (MQ=255)                        cGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTgg                                                                                                               <  2:652317/140‑1 (MQ=255)                        cGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTgg                                                                                                               <  2:701111/140‑1 (MQ=255)                          ccGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTa                                                                                                             <  1:336203/140‑1 (MQ=255)                          ccGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTa                                                                                                             <  2:896978/140‑1 (MQ=255)                            gTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAAt                                                                                                           <  1:94379/140‑1 (MQ=255)                              tAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGa                                                                                                         <  2:55876/140‑1 (MQ=255)                                   aGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGAt                                                                                                    >  2:87849/1‑140 (MQ=255)                                     cACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAg                                                                                                  <  2:279558/140‑1 (MQ=255)                                     cACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAg                                                                                                  <  1:915522/140‑1 (MQ=255)                                     cACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAg                                                                                                  <  1:864503/140‑1 (MQ=255)                                        gCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAgcgc                                                                                               >  2:592740/1‑140 (MQ=255)                                          tAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAgcgcgc                                                                                             >  2:288218/1‑140 (MQ=255)                                           aaTAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCt                                                                                            <  2:281029/140‑1 (MQ=255)                                             tAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCa                                                                                          <  1:938321/140‑1 (MQ=255)                                               gaagaaCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAAc                                                                                        >  2:847426/1‑140 (MQ=255)                                                aagaaCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAAcc                                                                                       <  1:566064/140‑1 (MQ=255)                                                  gaaCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAg                                                                                     >  2:1000290/1‑140 (MQ=255)                                                  gaaCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAg                                                                                     <  1:39079/140‑1 (MQ=255)                                                  gaaCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAg                                                                                     >  1:104733/1‑140 (MQ=255)                                                   aaCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAgc                                                                                    >  1:1029881/1‑140 (MQ=255)                                                    aCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCt                                                                                            >  1:400503/1‑131 (MQ=255)                                                    aCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCt                                                                                            <  2:400503/131‑1 (MQ=255)                                                    aCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAgcg                                                                                   >  1:811994/1‑140 (MQ=255)                                                    aCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAgcg                                                                                   >  2:982872/1‑140 (MQ=255)                                                    aCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAgcg                                                                                   >  2:1137922/1‑140 (MQ=255)                                                     cgcgAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAgcgc                                                                                  <  2:335604/140‑1 (MQ=255)                                                      gcgAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGcc                                                                                 >  1:610074/1‑140 (MQ=255)                                                      gcgAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGcc                                                                                 >  1:776735/1‑140 (MQ=255)                                                       cgAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCa                                                                                <  2:370772/140‑1 (MQ=255)                                                             ccgaccgaTAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCtt                                                                          >  2:674570/1‑140 (MQ=255)                                                                  cgaTAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTtcgtcg                                                                     <  1:653685/140‑1 (MQ=255)                                                                  cgaTAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTtcgtcg                                                                     <  2:104733/140‑1 (MQ=255)                                                                  cgaTAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTtcgtcg                                                                     <  1:321284/140‑1 (MQ=255)                                                                        taCGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCa                                                               <  2:886793/140‑1 (MQ=255)                                                                         aCGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCaa                                                              <  2:584016/140‑1 (MQ=255)                                                                         aCGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCaa                                                              <  1:288218/140‑1 (MQ=255)                                                                         aCGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCaa                                                              <  1:1137922/140‑1 (MQ=255)                                                                            gAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCaa                                                              <  1:291823/137‑1 (MQ=255)                                                                            gAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCaa                                                              >  2:291823/1‑137 (MQ=255)                                                                              acacGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGt                                                         >  1:267173/1‑140 (MQ=255)                                                                              acacGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGt                                                         <  2:1104579/140‑1 (MQ=255)                                                                               cacGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGtt                                                        >  2:229656/1‑140 (MQ=255)                                                                               cacGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGtt                                                        >  2:658004/1‑140 (MQ=255)                                                                               cacGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGtt                                                        >  2:746302/1‑140 (MQ=255)                                                                                  gTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTgg                                                     >  1:766930/1‑140 (MQ=255)                                                                                  gTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTgg                                                     <  1:262331/140‑1 (MQ=255)                                                                                   tCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTggg                                                    <  1:87849/140‑1 (MQ=255)                                                                                      tGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAg                                                 >  1:549602/1‑140 (MQ=255)                                                                                             ctggcGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCa                                          >  1:852628/1‑140 (MQ=255)                                                                                                gcgcTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAAc                                       <  1:658004/140‑1 (MQ=255)                                                                                                  gcTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATaa                                             <  1:221494/132‑1 (MQ=255)                                                                                                  gcTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATaa                                             >  2:221494/1‑132 (MQ=255)                                                                                                  gcTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGc                                     <  1:1000290/140‑1 (MQ=255)                                                                                                  gcTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGc                                     <  2:85376/140‑1 (MQ=255)                                                                                                   cTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGcc                                    <  1:674570/140‑1 (MQ=255)                                                                                                     tCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGcccc                                  <  2:907639/140‑1 (MQ=255)                                                                                                      cGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCt                                 >  2:724708/1‑140 (MQ=255)                                                                                                      cGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCt                                 >  1:681822/1‑140 (MQ=255)                                                                                                       gCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCtt                                >  2:219188/1‑140 (MQ=255)                                                                                                                  cGGTCGTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGgcg                     <  1:847426/140‑1 (MQ=255)                                                                                                                       gTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGa                 >  2:684174/1‑139 (MQ=255)                                                                                                                       gTTGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGa                 <  1:684174/139‑1 (MQ=255)                                                                                                                        ttGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACg               >  2:1053726/1‑140 (MQ=255)                                                                                                                         tGGGCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACgg              <  1:592740/140‑1 (MQ=255)                                                                                                                          gggCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGgc             <  2:549602/140‑1 (MQ=255)                                                                                                                           ggCCGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGgca            <  2:383217/140‑1 (MQ=255)                                                                                                                             ccGTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGgcagc          >  1:884136/1‑140 (MQ=255)                                                                                                                               gTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCTTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCAt        <  2:776735/140‑1 (MQ=255)                                                                                                                               gTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCAt        >  1:21984/1‑140 (MQ=255)                                                                                                                               gTTAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCAt        >  1:1106531/1‑140 (MQ=255)                                                                                                                                ttAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCCCTGCTTCGTCGATATCAACCTGTTATGTGTGTTAGTCACACGCCCATTGCTGTTAGGCGCTGTCGGCAGCGTc       <  2:349810/140‑1 (MQ=255)                                                                                                                                 tAAGCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATcc      <  1:746302/140‑1 (MQ=255)                                                                                                                                    gCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGa   <  2:450464/140‑1 (MQ=255)                                                                                                                                    gCTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGa   <  2:852628/140‑1 (MQ=255)                                                                                                                                     cTGAGCATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGAt  >  1:612644/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGAT  >  NC_000913/4054751‑4055022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑