| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,399,552 | A→G | I101V (ATC→GTC) | miaA → | tRNA dimethylallyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,399,552 | 0 | A | G | 97.7% | 151.3 / ‑4.0 | 43 | I101V (ATC→GTC) | miaA | tRNA dimethylallyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (9/33); total (9/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01 | |||||||||||
CGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCA > NC_000913/4399415‑4399688 | cGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTc > 2:213541/1‑140 (MQ=255) gAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTcc < 1:280885/140‑1 (MQ=255) aCGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTa < 2:499856/140‑1 (MQ=255) gCTGAAGAGTCACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGAAATCACCGCGGCGGGGCGGATCCCACTGTTAGt < 1:1058769/140‑1 (MQ=255) tGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTgg < 1:371232/140‑1 (MQ=255) aaGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGGCCCCCCGTTTGTgggg > 2:592532/1‑139 (MQ=255) agagTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCg < 2:992573/140‑1 (MQ=255) gagTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCgg < 2:1103418/140‑1 (MQ=255) gTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTa < 2:507355/140‑1 (MQ=255) gTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTa < 1:435268/140‑1 (MQ=255) ttACTCGCCGCGCCGCACCGATTGTTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGGAc > 1:905893/1‑140 (MQ=255) ttACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGGCCCACAGTTAATGGGGGGGAc > 1:1095313/1‑140 (MQ=255) tACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACg < 1:110146/140‑1 (MQ=255) aCTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGa < 1:413735/140‑1 (MQ=255) aCTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGa < 1:629497/140‑1 (MQ=255) aCTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGGGCCCCTGTTTGTggggggggcga > 1:586237/1‑135 (MQ=255) tCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGAtg < 1:225352/140‑1 (MQ=255) cgccgcgccgCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGGCCCCCCGTTTGTGGGGGGGGCGAtgt > 2:1071933/1‑140 (MQ=255) ccgcgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGAtgttg < 2:577417/140‑1 (MQ=255) cgcgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCCCTGTTTGTGGGGGGGACGAtgttgt > 1:740480/1‑140 (MQ=255) cgcgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGAtgttgt < 1:43819/140‑1 (MQ=255) cgcgccgcACCGATTGCTGGATATTCGCGATCCGTCACAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTa > 1:523500/1‑131 (MQ=255) cgcgccgcACCGATTGCTGGATATTCGCGATCCGTCACAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTa < 2:523500/131‑1 (MQ=255) gcgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGGCCCCCCGTTAGGTGGGGGGGCGAtgttgtt > 2:435268/1‑139 (MQ=255) gccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGGCCCCCCGGTAGTGGGGGGGACCAAGGTGTAtt > 2:43819/1‑140 (MQ=255) aCCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAg < 2:325443/140‑1 (MQ=255) ccGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAgg < 1:213541/140‑1 (MQ=255) ccGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAgg < 1:1117367/140‑1 (MQ=255) ccGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAgg < 2:1123399/140‑1 (MQ=255) gATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGTGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCa < 1:27107/140‑1 (MQ=255) aTTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCAt < 1:1130397/140‑1 (MQ=255) aCTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCa < 2:848182/140‑1 (MQ=255) tCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGa < 1:684748/140‑1 (MQ=255) ctgctgATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGGCCCCCTGTTTGTGGGGGGGACGATGTTGTATTTTAAAGCATTTCTGGGAGGGGTGTCGCCCCCACCGTCGGCAGAcccc > 2:8430/1‑139 (MQ=255) ctgctgATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGGCCCCCTGTTTGTGGGGGGGACGAAGTTGTATTTTAAAGGATTTCTGGGAGGGGTGTTGCCGCCACCCTCGGGAGAaccc > 2:463432/1‑139 (MQ=255) aTTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTa < 1:592532/140‑1 (MQ=255) aTTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTa < 1:463432/140‑1 (MQ=255) ccgcGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCa < 1:8430/140‑1 (MQ=255) aaTGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAggcgg < 2:307482/140‑1 (MQ=255) aaTGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAggcgg < 2:586237/140‑1 (MQ=255) aaTGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAggcgg < 1:768103/140‑1 (MQ=255) aaTGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAggcgg < 2:944983/140‑1 (MQ=255) ccGATATCACCGCGGCGGGGCGGGGCCCCCTGGTTGTGGGGGGGACGGTGTTGTATTTTAAGGGATTGCTGGGAGGGGTGTCGCCGCCACCCTCGGGCGACCCGGGAGTACGGGCCCGAATTGAGCAAAAGGGGGGagag > 2:69824/1‑140 (MQ=255) cGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGc < 2:963594/140‑1 (MQ=255) aCCGCGGCGGGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTg < 1:220640/140‑1 (MQ=255) cgcgGCGGGGCGGGGCCCCCCGTTTGTGGGGGGGACGATGTTGTATTTTAAGGCCTTGCTGGAAGGGGTGTCGCCCCCACCGGCGGCAGACCCGGAAGTACGGGGCAGAAATTAGCAACAAGCGGCAGAGCAAAGGTggg > 2:425602/1‑140 (MQ=255) cggcggGGCGGGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAg < 1:214451/140‑1 (MQ=255) cggGTCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCa < 1:1071933/140‑1 (MQ=255) | CGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCA > NC_000913/4399415‑4399688 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |