Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,448,745	T→A	D18E (GAT→GAA)	chpB →	endoribonuclease toxin ChpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,448,745	0	T	A	100.0%	227.5 / NA	65	D18E (GAT→GAA)	chpB	endoribonuclease toxin ChpB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (33/32);  total (33/32)

GCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTT  >  NC_000913/4448611‑4448880                                                                                                                                       |                                                                                                                                        gctgGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGc                                                                                                                                    >  2:894097/1‑140 (MQ=255)    ggCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAg                                                                                                                                 <  1:714826/140‑1 (MQ=255)    ggCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAg                                                                                                                                 <  1:894097/140‑1 (MQ=255)         aGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGcc                                                                                                                            <  1:33417/140‑1 (MQ=255)         aGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGcc                                                                                                                            <  1:960901/140‑1 (MQ=255)         aGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGcc                                                                                                                            <  1:842636/140‑1 (MQ=255)            gtgACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATg                                                                                                                         >  2:485081/1‑140 (MQ=255)             tgACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGa                                                                                                                        >  1:1040540/1‑140 (MQ=255)                   gAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCa                                                                                                                  >  1:817103/1‑140 (MQ=255)                   gAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCa                                                                                                                  <  2:377193/140‑1 (MQ=255)                   gAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCa                                                                                                                  <  1:847427/140‑1 (MQ=255)                   gAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCa                                                                                                                  <  1:68759/140‑1 (MQ=255)                     aCGCCGCGGAACTTAGCGAGCGGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAg                                                                                                                >  2:806373/1‑140 (MQ=255)                     aCGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATTCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGc                                                                                                                             <  2:573546/127‑1 (MQ=255)                     aCGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGc                                                                                                                             >  1:573546/1‑127 (MQ=255)                     aCGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAg                                                                                                                >  1:604257/1‑140 (MQ=255)                     aCGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAg                                                                                                                >  2:38480/1‑140 (MQ=255)                     aCGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAg                                                                                                                >  1:438582/1‑140 (MQ=255)                      cgccgcGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAgg                                                                                                               <  1:485081/140‑1 (MQ=255)                       gccgcGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCa                                                                                                                  <  2:408817/136‑1 (MQ=255)                       gccgcGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCa                                                                                                                  >  1:408817/1‑136 (MQ=255)                         cgcgGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGc                                                                                                            <  2:438582/140‑1 (MQ=255)                             gAACTTAGCGAGTAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGt                                                                                                        <  2:6186/140‑1 (MQ=255)                             gAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGt                                                                                                        <  2:163266/140‑1 (MQ=255)                               aCTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCg                                                                                                      <  2:342208/140‑1 (MQ=255)                                  tAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGAcc                                                                                                   <  2:385505/140‑1 (MQ=255)                                        gCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCt                                                                                             >  2:883927/1‑140 (MQ=255)                                           ggATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTtgt                                                                                          <  1:780376/140‑1 (MQ=255)                                           ggATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTtgt                                                                                          >  1:516458/1‑140 (MQ=255)                                                   ggggTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCg                                                                                  >  2:799625/1‑140 (MQ=255)                                                      gTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTc                                                                               <  1:266006/140‑1 (MQ=255)                                                      gTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTc                                                                               >  2:178249/1‑140 (MQ=255)                                                       tAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCa                                                                              <  1:690466/140‑1 (MQ=255)                                                          aTCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCTAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGc                                                                           >  2:78489/1‑140 (MQ=255)                                                          aTCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGc                                                                           >  2:78315/1‑140 (MQ=255)                                                          aTCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGc                                                                           <  2:1079692/140‑1 (MQ=255)                                                                   tGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCa                                                                  >  2:871143/1‑140 (MQ=255)                                                                      gggTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACt                                                               <  1:883927/140‑1 (MQ=255)                                                                       ggTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTg                                                              >  1:452210/1‑140 (MQ=255)                                                                       ggTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCATTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTg                                                              >  2:785090/1‑140 (MQ=255)                                                                                atatGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACg                                                     <  1:78315/140‑1 (MQ=255)                                                                                   tGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCtg                                                  <  1:799625/140‑1 (MQ=255)                                                                                      tAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCtggtg                                               >  1:956230/1‑140 (MQ=255)                                                                                        aaGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCTAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGc                                             <  1:78489/140‑1 (MQ=255)                                                                                        aaGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGc                                             <  2:817103/140‑1 (MQ=255)                                                                                        aaGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGc                                             <  1:871143/140‑1 (MQ=255)                                                                                        aaGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGc                                             >  1:824750/1‑140 (MQ=255)                                                                                        aaGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGc                                             <  2:604257/140‑1 (MQ=255)                                                                                         aGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGcc                                            >  2:154440/1‑140 (MQ=255)                                                                                         aGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGcc                                            >  1:459835/1‑140 (MQ=255)                                                                                              aGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCAt                                       >  2:740699/1‑140 (MQ=255)                                                                                                tGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTa                                     >  1:1135347/1‑140 (MQ=255)                                                                                                                 aCATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAAtttt                    <  2:75186/140‑1 (MQ=255)                                                                                                                 aCATTGTGCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAAtttt                    <  1:678871/140‑1 (MQ=255)                                                                                                                     tgtgCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGccc                >  1:667550/1‑140 (MQ=255)                                                                                                                       tgCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGt              >  2:754527/1‑140 (MQ=255)                                                                                                                       tgCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGt              >  2:207984/1‑140 (MQ=255)                                                                                                                        gCTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGtt             <  2:1045495/140‑1 (MQ=255)                                                                                                                         cTGGTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTa            >  2:418894/1‑140 (MQ=255)                                                                                                                            gTTGGCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGc         >  1:464567/1‑140 (MQ=255)                                                                                                                               ggCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTTCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCgg      >  2:263671/1‑140 (MQ=255)                                                                                                                               ggCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCgg      <  2:1135347/140‑1 (MQ=255)                                                                                                                                gCTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGa     <  2:516458/140‑1 (MQ=255)                                                                                                                                 cTTTGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGAt    >  2:693164/1‑140 (MQ=255)                                                                                                                                   ttGAACCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGAttt  >  2:82037/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        GCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTT  >  NC_000913/4448611‑4448880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑