Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,452,023	A→G	K322K (AAA→AAG)	ytfR →	galactofuranose ABC transporter putative ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,452,023	0	A	G	100.0%	186.8 / NA	50	K322K (AAA→AAG)	ytfR	galactofuranose ABC transporter putative ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/31);  total (19/31)

TGATCTCGAAGTACGCCCCGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAACCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCG  >  NC_000913/4451894‑4452141                                                                                                                                  |                                                                                                                       tGATCTCGAAGTACGCCCCGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAAcc                                                                                                              >  1:890048/1‑140 (MQ=255) tGATCTCGAAGTACGCCCCGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAAcc                                                                                                              >  1:626524/1‑140 (MQ=255)     ctcGAAGTACGCCCCGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCg                                                                                                          <  1:191716/140‑1 (MQ=255)       cGAAGTACGCCCCGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGAt                                                                                                        >  2:425497/1‑140 (MQ=255)         aaGTACGCCCCGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCg                                                                                                      >  2:115331/1‑140 (MQ=255)               gCCCCGGCGGGATGGTCGGTCTGGCTGGATTGGTGGGATCAGGACGTCCCGAAGCCGCCGAAGTGATCTTCGGTATCAAACCTGCTGCCAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACAt                                                                                                <  2:845102/140‑1 (MQ=255)               gCCCCGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACAt                                                                                                <  2:944570/140‑1 (MQ=255)                ccccGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATc                                                                                               >  1:466314/1‑140 (MQ=255)                 cccGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCa                                                                                              <  2:626524/140‑1 (MQ=255)                 cccGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCa                                                                                              <  1:966111/140‑1 (MQ=255)                  ccGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAg                                                                                             >  1:704086/1‑140 (MQ=255)                  ccGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAg                                                                                             >  1:1132455/1‑140 (MQ=255)                   cGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACAt                                                                                                >  2:928212/1‑136 (MQ=255)                   cGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACAt                                                                                                <  1:928212/136‑1 (MQ=255)                    ggCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGc                                                                                           >  1:636517/1‑140 (MQ=255)                     gCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCt                                                                                          >  1:201561/1‑140 (MQ=255)                      ggagaTCGTCGGTCTGGCTGGTTTGCTGGGATCAGGACGTCCCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCtt                                                                                         <  2:201561/139‑1 (MQ=255)                      cGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCtt                                                                                         <  1:438446/140‑1 (MQ=255)                      cGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCtt                                                                                         <  2:890048/140‑1 (MQ=255)                           tggtcgGCCTGGTTGGATTGCTGGAATCGGAACGTCCCGAAACCGCCGAAGTGATCTTCGGTATCAACCCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTa                                                                                    <  2:42113/138‑1 (MQ=255)                             gtcgGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACt                                                                                  <  1:155503/140‑1 (MQ=255)                               cgGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTgg                                                                                >  2:340893/1‑140 (MQ=255)                               cgGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTgg                                                                                >  1:726682/1‑140 (MQ=255)                                  tcgggttggATTGCTGGGATCGGGACGTCCCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCa                                                                             <  2:704086/134‑1 (MQ=255)                                  tCTGGCTGGATTGCTGGGATCAGGACGTCCCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCa                                                                             <  2:1022591/140‑1 (MQ=255)                                  tCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCa                                                                             <  1:1028594/140‑1 (MQ=255)                                      gctggATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTgg                                                                         >  1:741047/1‑140 (MQ=255)                                         ggATTGCTGGGATCGGGACGTCCCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCtt                                                                      <  2:593646/140‑1 (MQ=255)                                         ggATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCtt                                                                      <  1:308456/140‑1 (MQ=255)                                           aTTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGTGCATTGGCTTCt                                                                    <  1:115331/140‑1 (MQ=255)                                            ttGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACt                                                                                  <  2:833008/125‑1 (MQ=255)                                            ttGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACt                                                                                  >  1:833008/1‑125 (MQ=255)                                              gCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGcc                                                                 <  1:1039308/140‑1 (MQ=255)                                                tGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCg                                                               >  2:873878/1‑140 (MQ=255)                                                tGGGACCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAATCCGGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCg                                                               <  2:1020357/140‑1 (MQ=255)                                                     tCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGa                                                          <  1:340893/140‑1 (MQ=255)                                                             tACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGACAGGaaaa                                                  <  2:659535/140‑1 (MQ=255)                                                              aCCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGACAGGAAAAc                                                 >  2:528195/1‑140 (MQ=255)                                                               ccGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGACAGGAAAAcc                                                <  2:741047/140‑1 (MQ=255)                                                                cGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGACAGGAAAACCg                                               <  1:396437/140‑1 (MQ=255)                                                                 gAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGACAGGAAAACCGa                                              <  2:636517/140‑1 (MQ=255)                                                                   aaCCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAg                                                           >  2:613914/1‑125 (MQ=255)                                                                   aaCCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAg                                                           <  1:613914/125‑1 (MQ=255)                                                                                     cGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCt                          <  1:967420/140‑1 (MQ=255)                                                                                          tCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTg                     <  2:1132455/140‑1 (MQ=255)                                                                                                         gCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGc                    >  1:911856/1‑126 (MQ=255)                                                                                                         gCGGCACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGc                    <  2:911856/126‑1 (MQ=255)                                                                                                             cACGGCGTTGATCAAAGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTTGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCg  <  2:726682/140‑1 (MQ=255)                                                                                                                            aGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCg  <  2:798585/125‑1 (MQ=255)                                                                                                                            aGGCAAGCCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCg  >  1:798585/1‑125 (MQ=255)                                                                                                                                  |                                                                                                                       TGATCTCGAAGTACGCCCCGGCGAGATCGTCGGTCTGGCTGGATTGCTGGGATCAGGACGTACCGAAACCGCCGAAGTGATCTTCGGTATCAAACCTGCTGACAGCGGCACGGCGTTGATCAAAGGCAAACCGCAAAACCTGCGATCGCCACATCAGGCTTCGGTACTGGGCATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCG  >  NC_000913/4451894‑4452141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑