Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,454,722	A→G	R296R (CGT→CGC)	fbp ←	fructose‑1,6‑bisphosphatase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,454,722	0	A	G	100.0%	243.9 / NA	65	R296R (CGT→CGC)	fbp	fructose‑1,6‑bisphosphatase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/28);  total (37/28)

TCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGC  >  NC_000913/4454588‑4454857                                                                                                                                       |                                                                                                                                        tcCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGctc                                                                                                                                    >  1:1110017/1‑140 (MQ=255)  ccGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGctct                                                                                                                                   <  1:359012/140‑1 (MQ=255)   cGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCtt                                                                                                                                  <  2:72072/140‑1 (MQ=255)        ggAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCa                                                                                                                             >  1:205063/1‑140 (MQ=255)          agagGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCAtc                                                                                                                           <  1:157739/140‑1 (MQ=255)           gagGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCAtcg                                                                                                                          >  2:745006/1‑140 (MQ=255)           gagGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCAtcg                                                                                                                          <  2:728074/140‑1 (MQ=255)             ggCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCAtcgct                                                                                                                        >  1:330360/1‑140 (MQ=255)             ggCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGAATGATATCCAGAATGCGCTCTTTGCCAtcgct                                                                                                                        >  1:494183/1‑140 (MQ=255)              gcgcAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCAtcgctc                                                                                                                       >  1:678143/1‑140 (MQ=255)                 cAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATcgctcgct                                                                                                                    >  1:354170/1‑140 (MQ=255)                    gagaTTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTa                                                                                                                 >  2:968814/1‑140 (MQ=255)                        ttACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTAccgc                                                                                                             >  2:278576/1‑140 (MQ=255)                              gTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCtt                                                                                                       >  2:929944/1‑140 (MQ=255)                                ccGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGt                                                                                                     >  1:69745/1‑140 (MQ=255)                                 cGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGtt                                                                                                    >  1:575645/1‑140 (MQ=255)                                          aCGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGa                                                                                           >  2:847021/1‑140 (MQ=255)                                           cGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGaa                                                                                          <  1:968814/140‑1 (MQ=255)                                           cGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCACTTTACCGCCCGCTTGTTCCGCCAGGaa                                                                                          <  1:494324/140‑1 (MQ=255)                                                  aGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATc                                                                                   <  2:575645/140‑1 (MQ=255)                                                  aGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATc                                                                                   >  2:1087495/1‑140 (MQ=255)                                                    cGTTCGACATCTTCAACCATATGGTCGTTGCCGAGAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCACTTGTTCCGCCAGGAATGCCATCgg                                                                                 >  2:1007271/1‑140 (MQ=255)                                                    cGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCgg                                                                                 <  1:496652/140‑1 (MQ=255)                                                      ttCGTCATCTTCATCCATATGTTCGTTGCGAACAAGGATTGAAGGGCGTTGGTGCAGGGTTTCCGTGATGATATGCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGt                                                                               <  2:861504/140‑1 (MQ=255)                                                      ttCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATc                                                                                   <  1:1082272/136‑1 (MQ=255)                                                      ttCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATc                                                                                   >  2:1082272/1‑136 (MQ=255)                                                      ttCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGt                                                                               <  1:847021/140‑1 (MQ=255)                                                      ttCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGt                                                                               >  2:496230/1‑140 (MQ=255)                                                        cGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTg                                                                             <  2:652496/140‑1 (MQ=255)                                                                       tatGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCaaa                                                              >  1:882630/1‑140 (MQ=255)                                                                         tGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCa                                                                           <  1:533929/125‑1 (MQ=255)                                                                         tGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCa                                                                           >  2:533929/1‑125 (MQ=255)                                                                          ggTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATAc                                                                    <  2:366325/131‑1 (MQ=255)                                                                          ggTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATAc                                                                    >  1:366325/1‑131 (MQ=255)                                                                              gTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGtt                                                       >  1:23072/1‑140 (MQ=255)                                                                              gTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGtt                                                       >  1:9350/1‑140 (MQ=255)                                                                              gTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGtt                                                       >  2:557526/1‑140 (MQ=255)                                                                                 gCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGc                                                    >  1:277676/1‑140 (MQ=255)                                                                                     aCAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCa                                                          <  1:1113834/130‑1 (MQ=255)                                                                                     aCAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCa                                                          >  2:1113834/1‑130 (MQ=255)                                                                                        aaGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCgg                                             <  2:69745/140‑1 (MQ=255)                                                                                         aGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCggg                                            >  1:72886/1‑140 (MQ=255)                                                                                          gAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGt                                           <  1:566850/140‑1 (MQ=255)                                                                                             tGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggc                                        <  1:745006/140‑1 (MQ=255)                                                                                                aCGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggctgg                                     <  2:9350/140‑1 (MQ=255)                                                                                                aCGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggctgg                                     <  2:882630/140‑1 (MQ=255)                                                                                                aCGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggctgg                                     >  2:118909/1‑140 (MQ=255)                                                                                                aCGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCACTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggctgg                                     <  1:1007271/140‑1 (MQ=255)                                                                                                 cGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggctggc                                    >  2:522863/1‑140 (MQ=255)                                                                                                             aGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTa                        <  1:496230/140‑1 (MQ=255)                                                                                                             aGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTa                        <  2:277676/140‑1 (MQ=255)                                                                                                             aGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTa                        <  2:205063/140‑1 (MQ=255)                                                                                                             aGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTa                        <  2:1040320/140‑1 (MQ=255)                                                                                                             aGGGTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTa                        <  2:1110017/140‑1 (MQ=255)                                                                                                              gggTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGc                               >  2:388736/1‑132 (MQ=255)                                                                                                              gggTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGc                               >  1:157300/1‑132 (MQ=255)                                                                                                              gggTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGc                               <  2:157300/132‑1 (MQ=255)                                                                                                              gggTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGc                               <  1:388736/132‑1 (MQ=255)                                                                                                              gggTTTCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTag                       >  2:685282/1‑140 (MQ=255)                                                                                                                 tttCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAgaga                    >  1:199443/1‑140 (MQ=255)                                                                                                                   tCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATa                  >  1:664099/1‑140 (MQ=255)                                                                                                                   tCCGGGATGATATCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATa                  >  2:953592/1‑140 (MQ=255)                                                                                                                            atatCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGcc         >  2:889139/1‑140 (MQ=255)                                                                                                                             tatCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCt        >  1:837330/1‑140 (MQ=255)                                                                                                                              atCCAGAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCtt       >  1:1121483/1‑140 (MQ=255)                                                                                                                                   gAATGCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTcagc  <  2:504311/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGC  >  NC_000913/4454588‑4454857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑