Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,589,904	A→G	V459A (GTC→GCC)	btsT ←	pyruvate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,589,904	0	A	G	100.0%	215.7 / NA	58	V459A (GTC→GCC)	btsT	pyruvate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/26);  total (32/26)

TCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAGCTGCGCCATGATGATCGGCGCGT  >  NC_000913/4589773‑4590033                                                                                                                                    |                                                                                                                                   tCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGc                                                                                                                            <  1:694063/140‑1 (MQ=255)           cAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGt                                                                                                                  >  1:543906/1‑140 (MQ=255)                cTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTc                                                                                                             >  2:68139/1‑140 (MQ=255)                   cGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTcgc‑g                                                                                                         <  2:864409/140‑1 (MQ=255)                   cGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTcgc‑g                                                                                                         >  1:35795/1‑140 (MQ=255)                   cGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTcgc‑g                                                                                                         >  2:486690/1‑140 (MQ=255)                   cGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCCCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTcgc‑g                                                                                                         <  2:790453/140‑1 (MQ=255)                    gAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑gg                                                                                                        <  1:626578/140‑1 (MQ=255)                    aaaCAGAATCCCAAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGTGCAGAAGGCTCACCAATGTCTTTCGC‑gg                                                                                                        <  2:255518/139‑1 (MQ=255)                        aGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTg                                                                                                    <  2:543906/140‑1 (MQ=255)                               cGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATc                                                                                             >  1:758998/1‑140 (MQ=255)                                  aGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGc                                                                                          >  2:691867/1‑140 (MQ=255)                                    tGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGctc                                                                                        >  2:868201/1‑140 (MQ=255)                                    tGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGctc                                                                                        >  2:416451/1‑140 (MQ=255)                                    tGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGctc                                                                                        >  1:37493/1‑140 (MQ=255)                                     gtatccAGAGTCCCATGTCGGCTATCGGTAGAACTTTTTGTAACACGGGCACCGTCCCGACCGCCAGCGTCGGCGCGCCCCCGGCACGGTTCAGGGCGGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTActct                                                                                       <  2:1104054/136‑1 (MQ=255)                                      aTACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTg                                                                                      >  2:389840/1‑140 (MQ=255)                                        aCCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGc                                                                                    >  2:488971/1‑140 (MQ=255)                                        aCCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCACCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGc                                                                                    >  2:48028/1‑140 (MQ=255)                                           aGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGaa                                                                                 >  1:883312/1‑140 (MQ=255)                                            gAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGaaa                                                                                >  1:762447/1‑140 (MQ=255)                                                cccATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGTTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCAc                                                                            <  2:1013206/140‑1 (MQ=255)                                                cccATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGAGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCAc                                                                            >  2:1057198/1‑140 (MQ=255)                                                  cATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACg                                                                           <  2:960341/139‑1 (MQ=255)                                                  cATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACg                                                                           >  1:960341/1‑139 (MQ=255)                                                  cATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGa                                                                          <  2:758998/140‑1 (MQ=255)                                                  cATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGa                                                                          >  2:639859/1‑140 (MQ=255)                                                  cATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGa                                                                          <  1:389840/140‑1 (MQ=255)                                                   aTGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGaa                                                                         >  1:441407/1‑140 (MQ=255)                                                             tCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAg                                                               >  2:620440/1‑140 (MQ=255)                                                               ggCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAg                                                                  >  2:172995/1‑135 (MQ=255)                                                               ggCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAg                                                                  <  1:172995/135‑1 (MQ=255)                                                                  agcaCTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGAc                                                          <  1:741327/140‑1 (MQ=255)                                                                         tgtgGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCt                                                   >  1:84934/1‑140 (MQ=255)                                                                           tgGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGc                                                 >  2:203613/1‑140 (MQ=255)                                                                            ggAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAGGCCCCAGGAGCTGACGGTCGCTGcc                                                <  1:416451/140‑1 (MQ=255)                                                                            ggAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGcc                                                <  1:891454/140‑1 (MQ=255)                                                                            ggAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGcc                                                <  1:868201/140‑1 (MQ=255)                                                                            ggAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGcc                                                <  2:84934/140‑1 (MQ=255)                                                                              aaCACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCcgc                                              <  2:441407/140‑1 (MQ=255)                                                                              aaCACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCcgc                                              >  2:355336/1‑140 (MQ=255)                                                                                cacGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGcc                                                >  1:627486/1‑136 (MQ=255)                                                                                cacGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGcc                                                <  2:627486/136‑1 (MQ=255)                                                                                cacGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGcc                                                >  1:3513/1‑136 (MQ=255)                                                                                cacGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGcc                                                <  2:3513/136‑1 (MQ=255)                                                                                   gTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGtgtg                                         <  1:488971/140‑1 (MQ=255)                                                                                   gTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGtgtg                                         >  1:89237/1‑140 (MQ=255)                                                                                   gTGCGCGATACCTACCACCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGtgtg                                         <  1:48028/140‑1 (MQ=255)                                                                                      cgcgATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCgg                                      <  2:89237/140‑1 (MQ=255)                                                                                        cgATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGcc                                                <  1:54125/128‑1 (MQ=255)                                                                                        cgATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGcc                                                >  2:54125/1‑128 (MQ=255)                                                                                        cgATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTa                                    <  1:1057198/140‑1 (MQ=255)                                                                                           tacctaccGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACg                                 <  2:1112358/140‑1 (MQ=255)                                                                                                   gCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAg                         >  2:90988/1‑140 (MQ=255)                                                                                                       gCGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAGCTgc                     >  2:524399/1‑140 (MQ=255)                                                                                                        cGTCGGCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAGCTgcg                    <  1:68139/140‑1 (MQ=255)                                                                                                            ggCGCGCCACCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGCGGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAGCTGCGCCa                 >  1:95561/1‑140 (MQ=255)                                                                                                                    aCCTGCACGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAGCTGCGCCATGATGATCg        >  2:639501/1‑140 (MQ=255)                                                                                                                          aCGGTTCAGGGCAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGTCGCGTGTGCGGTAACGTCTTTCAGCTGCGCCATGATGATCGGCGCGt  >  1:78833/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                   TCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGC‑GGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAGCTGCGCCATGATGATCGGCGCGT  >  NC_000913/4589773‑4590033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑