Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,068	C→T	R152H (CGT→CAT)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,068	0	C	T	100.0%	178.2 / NA	50	R152H (CGT→CAT)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/28);  total (22/28)

GGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACA  >  NC_000913/4629932‑4630203                                                                                                                                         |                                                                                                                                        ggATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTa                                                                                                                                      >  1:436655/1‑140 (MQ=255)      cGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCa                                                                                                                                 >  2:259672/1‑140 (MQ=255)          aTCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATccg                                                                                                                             <  2:1055083/140‑1 (MQ=255)          aTCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATccg                                                                                                                             <  1:259672/140‑1 (MQ=255)               ggtggtTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCa                                                                                                                        <  2:261362/140‑1 (MQ=255)                  ggttggtCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGc                                                                                                                     <  1:358133/140‑1 (MQ=255)                   gttggtCGGTTCGTCGAGCAGGAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCt                                                                                                                    >  2:805661/1‑140 (MQ=255)                     tggtCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTcc                                                                                                                  <  1:79271/140‑1 (MQ=255)                      ggtCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCa                                                                                                                 >  1:738593/1‑140 (MQ=255)                       gtCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGc                                                                                                                     <  1:930348/135‑1 (MQ=255)                       gtCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGc                                                                                                                     >  2:930348/1‑135 (MQ=255)                        tCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGc                                                                                                               >  2:465113/1‑140 (MQ=255)                              cgtcgAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACg                                                                                                         >  1:433333/1‑140 (MQ=255)                                tcgAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGtt                                                                                                       <  2:84732/140‑1 (MQ=255)                                tcgAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGtt                                                                                                       <  2:910353/140‑1 (MQ=255)                                       gcagcaTGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTa                                                                                                <  1:348923/140‑1 (MQ=255)                                           caTGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGAc                                                                                            <  1:1128904/140‑1 (MQ=255)                                             tGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCAGCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACcg                                                                                          >  1:871877/1‑140 (MQ=255)                                                cTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACcgtcg                                                                                       <  2:196000/140‑1 (MQ=255)                                                cTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACcgtcg                                                                                       <  2:173180/140‑1 (MQ=255)                                                cTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGAACGTTCAGATTATGACcgtcg                                                                                       <  2:494765/140‑1 (MQ=255)                                                 tGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCAGCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACcgtcgt                                                                                      <  2:871877/140‑1 (MQ=255)                                                     ttttCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGc                                                                                  <  1:805661/140‑1 (MQ=255)                                                      tttCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGcc                                                                                 >  2:646933/1‑140 (MQ=255)                                                         ccAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGc                                                                                  <  1:134779/136‑1 (MQ=255)                                                         ccAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGc                                                                                  >  2:134779/1‑136 (MQ=255)                                                          cagcagcagGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGaa                                                                             <  2:482855/140‑1 (MQ=255)                                                              agcagGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGAt                                                                         <  1:465113/140‑1 (MQ=255)                                                                  ggcggcACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATctct                                                                     >  1:108513/1‑140 (MQ=255)                                                                        acaACGCTACGCGACGCCGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGa                                                               <  2:1018574/140‑1 (MQ=255)                                                                        acaACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGa                                                               <  2:108513/140‑1 (MQ=255)                                                                                 cgcgACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGt                                                      >  1:205479/1‑140 (MQ=255)                                                                                      cgacgTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGc                                                 >  2:458677/1‑140 (MQ=255)                                                                                       gacgTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCg                                                >  1:210709/1‑140 (MQ=255)                                                                                             caccacCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGc                                          <  1:458677/140‑1 (MQ=255)                                                                                             caccacCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGc                                          <  2:433333/140‑1 (MQ=255)                                                                                                   cGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCa                                    >  2:56988/1‑140 (MQ=255)                                                                                                       gagGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAAt                                <  2:499191/140‑1 (MQ=255)                                                                                                        agGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATc                               >  1:802429/1‑140 (MQ=255)                                                                                                            tAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCa                           >  2:675840/1‑140 (MQ=255)                                                                                                            tAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCa                           >  1:128585/1‑140 (MQ=255)                                                                                                             aGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCAt                          >  2:196519/1‑140 (MQ=255)                                                                                                             aGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCAt                          >  2:143475/1‑140 (MQ=255)                                                                                                                     tCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCg                  >  2:165246/1‑140 (MQ=255)                                                                                                                           cccAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTAc            <  1:165246/140‑1 (MQ=255)                                                                                                                            ccAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACa           <  2:210709/140‑1 (MQ=255)                                                                                                                                 ccGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCa      <  2:205479/140‑1 (MQ=255)                                                                                                                                 ccGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCa      <  1:675840/140‑1 (MQ=255)                                                                                                                                  cGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCAt     <  1:196519/140‑1 (MQ=255)                                                                                                                                     cAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATaca  >  1:960379/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACA  >  NC_000913/4629932‑4630203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑