Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	542,589	A→G	Q234Q (CAA→CAG)	glxK →	glycerate 2‑kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	542,589	0	A	G	100.0%	158.1 / NA	43	Q234Q (CAA→CAG)	glxK	glycerate 2‑kinase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/24);  total (19/24)

GAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAAT  >  NC_000913/542452‑542718                                                                                                                                          |                                                                                                                                  gAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTacagca                                                                                                                                 <  2:552650/140‑1 (MQ=255)  aaGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCGCGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTAcagcag                                                                                                                                >  2:1103415/1‑140 (MQ=255)  aaGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTAcagcag                                                                                                                                >  1:498947/1‑140 (MQ=255)    gTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCGCGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAgcagca                                                                                                                              <  1:1103415/140‑1 (MQ=255)         aTGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTg                                                                                                                         <  1:610422/140‑1 (MQ=255)          tgtgACGTGGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGa                                                                                                                        >  1:793435/1‑140 (MQ=255)            tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGaaa                                                                                                                      >  1:851833/1‑140 (MQ=255)            tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGaaa                                                                                                                      >  2:414877/1‑140 (MQ=255)                   gATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGt                                                                                                               <  2:851833/140‑1 (MQ=255)                       aCCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGc                                                                                                           <  1:91793/140‑1 (MQ=255)                           gCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGa                                                                                                       >  2:471289/1‑140 (MQ=255)                           gCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGa                                                                                                       >  1:84771/1‑140 (MQ=255)                                   gggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATggcgggc                                                                                               >  1:452758/1‑140 (MQ=255)                                     gCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGgcgggcgg                                                                                             >  1:248520/1‑140 (MQ=255)                                         gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                                                                         >  1:481833/1‑140 (MQ=255)                                         gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                                                                         >  1:498703/1‑140 (MQ=255)                                         gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                                                                         >  2:1122768/1‑140 (MQ=255)                                              gcggcggcgGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgc                                                                                    <  2:84771/140‑1 (MQ=255)                                               cggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcg                                                                                   <  1:414877/140‑1 (MQ=255)                                               cggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcg                                                                                   <  2:481833/140‑1 (MQ=255)                                                ggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg                                                                                  <  1:595711/140‑1 (MQ=255)                                                 gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                                                                                 <  2:376723/140‑1 (MQ=255)                                                 gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                                                                                 <  1:526730/140‑1 (MQ=255)                                                 gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                                                                                 <  1:1122768/140‑1 (MQ=255)                                                 gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                                                                                 <  2:1030769/140‑1 (MQ=255)                                                  cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                                                                                <  2:498947/140‑1 (MQ=255)                                                  cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                                                                                <  2:970129/140‑1 (MQ=255)                                                                ccGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgc                                                                  <  1:940039/140‑1 (MQ=255)                                                                 cGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcg                                                                 >  2:793216/1‑140 (MQ=255)                                                                  gCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcgg                                                                >  1:85560/1‑140 (MQ=255)                                                                  gCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAACAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcgg                                                                >  1:130529/1‑140 (MQ=255)                                                                   cAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcggc                                                               >  2:792262/1‑140 (MQ=255)                                                                     aaaaGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcggcgg                                                             <  1:912074/140‑1 (MQ=255)                                                                          gggCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTa                                                        <  1:102698/140‑1 (MQ=255)                                                                                    ggAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATg                                              >  2:897304/1‑140 (MQ=255)                                                                                     gagaTGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGc                                             <  2:872654/140‑1 (MQ=255)                                                                                      agaTGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCg                                            <  2:248520/140‑1 (MQ=255)                                                                                          ggTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGata                                        >  1:929758/1‑140 (MQ=255)                                                                                           gTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGatat                                       >  1:104168/1‑140 (MQ=255)                                                                                                         aCAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTg                         <  1:792262/140‑1 (MQ=255)                                                                                                         aCAGGGGCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTg                         <  1:793216/140‑1 (MQ=255)                                                                                                             gggCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAAt                     <  2:793435/140‑1 (MQ=255)                                                                                                             gggCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAAt                     <  2:85560/140‑1 (MQ=255)                                                                                                             gggCTGCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAAt                     >  2:436368/1‑140 (MQ=255)                                                                                                                  gCAAAATTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTgtgt                >  1:668367/1‑140 (MQ=255)                                                                                                                      aaTTACGCCCGTGTTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGaa            <  1:436368/140‑1 (MQ=255)                                                                                                                                gtgtTTTACAGCAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAAt  <  2:452758/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  GAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAAT  >  NC_000913/542452‑542718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑