Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	78,395	A→G	T259A (ACG→GCG)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	78,395	0	A	G	100.0%	317.7 / NA	84	T259A (ACG→GCG)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/39);  total (45/39)

AGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAG  >  NC_000913/78258‑78528                                                                                                                                          |                                                                                                                                      aGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCg                                                                                                                                     >  1:973138/1‑140 (MQ=255) aGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCg                                                                                                                                     >  1:348488/1‑140 (MQ=255)   gATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGggcggc                                                                                                                                   <  1:326959/140‑1 (MQ=255)     tAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAg                                                                                                                                 <  2:115186/140‑1 (MQ=255)          aCGTACGGATGTTATTTGTCGTCTCGACGTTAATGTGGGCCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGctgga                                                                                                                            <  2:813726/140‑1 (MQ=255)             tACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTTCTGATGGGGGCGGCAGctgggctg                                                                                                                         >  1:7012/1‑140 (MQ=255)             tACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGctggactg                                                                                                                         >  2:571327/1‑140 (MQ=255)               cGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGctggactgga                                                                                                                       >  1:663790/1‑140 (MQ=255)               cGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGctggactgga                                                                                                                       <  2:348488/140‑1 (MQ=255)               cGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGctggactgga                                                                                                                       >  2:137342/1‑140 (MQ=255)               cGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGctggactgga                                                                                                                       >  2:868202/1‑140 (MQ=255)                ggATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGaa                                                                                                                      >  1:848381/1‑140 (MQ=255)                  aTGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAAt                                                                                                                    <  2:973138/140‑1 (MQ=255)                      tATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAAt                                                                                                                    >  1:828243/1‑136 (MQ=255)                      tATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAAt                                                                                                                    <  2:828243/136‑1 (MQ=255)                       aTTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGctgga                                                                                                                            >  2:121709/1‑127 (MQ=255)                       aTTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGctgga                                                                                                                            <  1:121709/127‑1 (MQ=255)                            tCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATg                                                                                                          >  2:225315/1‑140 (MQ=255)                            tCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGGTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATg                                                                                                          >  2:206588/1‑140 (MQ=255)                              gCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGAt                                                                                                        >  2:1077734/1‑140 (MQ=255)                              gCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGAt                                                                                                        >  2:269252/1‑140 (MQ=255)                               ccTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGAtt                                                                                                       >  2:309502/1‑140 (MQ=255)                                   gACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTctgg                                                                                                   <  1:304140/140‑1 (MQ=255)                                     cGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTctggct                                                                                                 <  1:269252/140‑1 (MQ=255)                                     cGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTctggct                                                                                                 <  2:797199/140‑1 (MQ=255)                                     cGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTctggct                                                                                                 <  1:821530/140‑1 (MQ=255)                                           tgtgGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGctac                                                                                           <  2:325727/140‑1 (MQ=255)                                            gtgGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGctact                                                                                          <  1:38368/140‑1 (MQ=255)                                               gACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCGGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATg                                                                                       >  2:653994/1‑140 (MQ=255)                                               gACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATg                                                                                       >  1:657869/1‑140 (MQ=255)                                               gACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATg                                                                                       >  2:365271/1‑140 (MQ=255)                                                   tGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCaa                                                                                   <  2:767392/140‑1 (MQ=255)                                                    gCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCaaa                                                                                  >  2:363098/1‑140 (MQ=255)                                                     cAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGctact                                                                                          >  1:674275/1‑131 (MQ=255)                                                     cAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGctact                                                                                          <  2:674275/131‑1 (MQ=255)                                                            aTGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATgg                                                                          >  2:725579/1‑140 (MQ=255)                                                             tGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGt                                                                         >  2:99488/1‑140 (MQ=255)                                                               tACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTaa                                                                       <  2:657869/140‑1 (MQ=255)                                                                 cATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAgc                                                                     >  2:380841/1‑140 (MQ=255)                                                                  attattGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAgcg                                                                    <  2:568795/140‑1 (MQ=255)                                                                       tGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGaa                                                               >  1:1020599/1‑140 (MQ=255)                                                                         atatGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGaacg                                                             <  1:571327/140‑3 (MQ=255)                                                                         atatGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAatg                                                             <  1:946115/140‑1 (MQ=255)                                                                         atatGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAatg                                                             <  1:375852/140‑1 (MQ=255)                                                                         atatGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAatg                                                             <  1:206588/140‑1 (MQ=255)                                                                         atatGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAatg                                                             <  2:328804/140‑1 (MQ=255)                                                                         atatGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAatg                                                             >  2:1037119/1‑140 (MQ=255)                                                                         atatGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAatg                                                             >  2:375852/1‑140 (MQ=255)                                                                         atatGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATATCAAACGTTATGGTAAGCGGCGAatg                                                             >  1:796147/1‑140 (MQ=255)                                                                            tGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAatgatg                                                          >  2:187799/1‑140 (MQ=255)                                                                              ccGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGGTGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGt                                                        >  1:404209/1‑140 (MQ=255)                                                                              ccGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGt                                                        >  2:1009730/1‑140 (MQ=255)                                                                                gTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGGAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCa                                                      >  2:233719/1‑140 (MQ=255)                                                                                 ttGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCAt                                                     <  1:868202/140‑1 (MQ=255)                                                                                   gtgGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAg                                                   <  1:363098/140‑1 (MQ=255)                                                                                   gtgGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAg                                                   >  1:177354/1‑140 (MQ=255)                                                                                     ggATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCa                                                 <  1:380841/140‑1 (MQ=255)                                                                                         cAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTgg                                             >  2:787909/1‑140 (MQ=255)                                                                                            tAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTggcgg                                          <  1:481798/140‑1 (MQ=255)                                                                                            tAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTggcgg                                          >  1:100019/1‑140 (MQ=255)                                                                                             aGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTggcggc                                         <  1:187799/140‑1 (MQ=255)                                                                                              gCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCa                                        >  1:106041/1‑140 (MQ=255)                                                                                                 aGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAggg                                     >  2:91992/1‑139 (MQ=255)                                                                                                 aGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGa                                     >  2:375076/1‑140 (MQ=255)                                                                                                 aGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACATTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAg                                       >  2:187942/1‑138 (MQ=255)                                                                                                 aGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACATTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAg                                       <  1:187942/138‑1 (MQ=255)                                                                                                  gTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAg                                    <  1:1009730/140‑1 (MQ=255)                                                                                                  gTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAg                                    <  2:663790/140‑1 (MQ=255)                                                                                                   ttAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGt                                   >  1:786343/1‑140 (MQ=255)                                                                                                    tAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTa                                  <  2:1020599/140‑1 (MQ=255)                                                                                                    tAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTa                                  <  2:744523/140‑1 (MQ=255)                                                                                                    tAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTa                                  <  1:1077734/140‑1 (MQ=255)                                                                                                    tAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTa                                  <  1:309502/140‑1 (MQ=255)                                                                                                     aGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTAc                                 <  1:787909/140‑1 (MQ=255)                                                                                                     aGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTAc                                 >  1:212736/1‑140 (MQ=255)                                                                                                     aGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGGAc                                 >  1:347668/1‑140 (MQ=255)                                                                                                      ggATTGCCAGACAAACTGGCGGGTTTCCTGTTGGGGGCGGCAGCTGGACTGGAAAAAACAGGAATGATTCTGGCTGGCTACTATGTCAAACCTTATGGGAAGCGGCCAATTATGGTCATAGCAGTGGCGGCAAGAGGACt                                >  2:419546/1‑140 (MQ=255)                                                                                                           gCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGtttt                           >  2:298713/1‑140 (MQ=255)                                                                                                           gCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGGACTGtttt                           >  2:180870/1‑140 (MQ=255)                                                                                                           gCCAGACAAACTGGCGGGGTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGgttt                           >  1:946899/1‑140 (MQ=255)                                                                                                            ccAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGGGGACTGTTTTa                          >  2:183445/1‑140 (MQ=255)                                                                                                            ccAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTa                          >  2:1029546/1‑140 (MQ=255)                                                                                                             cAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGGTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGGGGACTGttttta                         >  2:458840/1‑138 (MQ=255)                                                                                                                   aaCTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGga                   <  2:848381/140‑1 (MQ=255)                                                                                                                    aCTGGCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGgat                  <  1:72872/140‑1 (MQ=255)                                                                                                                       ggCGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGgattga               <  1:137342/140‑1 (MQ=255)                                                                                                                         cGGGTTTCCTGATGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGgattgatt             <  1:1037119/140‑1 (MQ=255)                                                                                                                                    aTGGGGGCGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAg  <  1:365271/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      AGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAG  >  NC_000913/78258‑78528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑