Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	548,703	A→G	N116S (AAC→AGC)	ylbE →	DUF1116 domain‑containing protein YlbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	548,703	0	A	G	100.0%	238.1 / NA	64	N116S (AAC→AGC)	ylbE	DUF1116 domain‑containing protein YlbE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/28);  total (36/28)

GGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCG  >  NC_000913/548568‑548837                                                                                                                                        |                                                                                                                                        gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTg                                                                                                                                     >  2:475383/1‑140 (MQ=255)  ggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGa                                                                                                                                    <  2:273346/140‑1 (MQ=255)               gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCg                                                                                                                       >  2:557522/1‑140 (MQ=255)                  cAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGc                                                                                                                    <  1:330559/140‑1 (MQ=255)                   aGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCa                                                                                                                           >  1:615885/1‑132 (MQ=255)                   aGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCa                                                                                                                           <  2:615885/132‑1 (MQ=255)                          ggCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCGGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCa                                                                                                            >  2:886632/1‑140 (MQ=255)                           gCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCaa                                                                                                           <  1:557522/140‑1 (MQ=255)                            cAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAAc                                                                                                          <  2:982681/140‑1 (MQ=255)                               tACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTc                                                                                                       >  2:651429/1‑140 (MQ=255)                                  tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAAc                                                                                                    >  1:6863/1‑140 (MQ=255)                                   ggAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACg                                                                                                   <  1:405366/140‑1 (MQ=255)                                     aGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGGGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGaa                                                                                                 >  1:710121/1‑140 (MQ=255)                                     aGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGaa                                                                                                 >  1:1071536/1‑140 (MQ=255)                                         gggggAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAAc                                                                                                          <  1:982779/127‑1 (MQ=255)                                         gggggAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAAc                                                                                                          >  2:982779/1‑127 (MQ=255)                                         gggggAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTa                                                                                             >  1:678037/1‑140 (MQ=255)                                         gggggAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTa                                                                                             >  2:1001966/1‑140 (MQ=255)                                         gggggAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTa                                                                                             >  1:441715/1‑140 (MQ=255)                                          ggggAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTAt                                                                                            >  2:527205/1‑140 (MQ=255)                                                     cTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGa                                                                                 >  1:319916/1‑140 (MQ=255)                                                        cATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGc                                                                              >  1:650052/1‑140 (MQ=255)                                                               tGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTgg                                                                      >  2:973061/1‑140 (MQ=255)                                                               tGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTgg                                                                      >  2:12208/1‑140 (MQ=255)                                                                 tCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTG‑‑ACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCt                                                                  <  2:39863/140‑1 (MQ=255)                                                                  cACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGgcgc                                                                   >  1:983490/1‑140 (MQ=255)                                                                       tgtgAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACg                                                              >  1:210794/1‑140 (MQ=255)                                                                       tgtgAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACg                                                              >  1:759689/1‑140 (MQ=255)                                                                         tgAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGc                                                            >  1:689835/1‑140 (MQ=255)                                                                           aaTGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTTGGCGCTTACGGCg                                                           >  1:416189/1‑140 (MQ=255)                                                                           aaTGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGa                                                          >  1:869649/1‑140 (MQ=255)                                                                            aTGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGgcg                                                                    <  1:1072587/129‑1 (MQ=255)                                                                            aTGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGgcg                                                                    >  2:1072587/1‑129 (MQ=255)                                                                            aTGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGaa                                                         <  2:755127/140‑1 (MQ=255)                                                                            aTGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGaa                                                         <  2:678037/140‑1 (MQ=255)                                                                               ccGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGAt                                                      >  2:163884/1‑140 (MQ=255)                                                                                cGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATg                                                     >  2:376339/1‑140 (MQ=255)                                                                                  tCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTc                                                   >  1:692095/1‑140 (MQ=255)                                                                                  tCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTc                                                   >  1:1103296/1‑140 (MQ=255)                                                                                  tCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTc                                                   <  1:651429/140‑1 (MQ=255)                                                                                  tCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTc                                                   >  2:75280/1‑140 (MQ=255)                                                                                         aaTGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTc                                            <  2:1030484/140‑1 (MQ=255)                                                                                               cGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATc                                      <  2:210794/140‑1 (MQ=255)                                                                                                 gTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGc                                    <  2:869649/140‑1 (MQ=255)                                                                                                       cTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATg                              <  2:675368/140‑1 (MQ=255)                                                                                                         tCTGCCAGTATGCCGATGCTGGTGGTTGGGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATgcg                            <  2:710121/140‑1 (MQ=255)                                                                                                         tCTGCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATgcg                            <  1:1001966/140‑1 (MQ=255)                                                                                                            gCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGa                         >  1:32410/1‑140 (MQ=255)                                                                                                            gCCAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGa                         >  1:207492/1‑140 (MQ=255)                                                                                                             ccAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGAt                        <  2:983490/140‑1 (MQ=255)                                                                                                             ccAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGAt                        <  2:32410/140‑1 (MQ=255)                                                                                                              cAGTATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGAtg                       <  1:653823/140‑1 (MQ=255)                                                                                                                 tATGCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATgtgt                    >  2:572806/1‑140 (MQ=255)                                                                                                                    gCCGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTaa                 <  1:12208/140‑1 (MQ=255)                                                                                                                      cGATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATg               <  2:759689/140‑1 (MQ=255)                                                                                                                       gATGCTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGc              <  1:163884/140‑1 (MQ=255)                                                                                                                           cTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGt          >  1:1029701/1‑140 (MQ=255)                                                                                                                           cTGGTGGTTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGAGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGt          >  1:652041/1‑140 (MQ=255)                                                                                                                              gtggtTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTaa                 <  2:569023/130‑1 (MQ=255)                                                                                                                              gtggtTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTaa                 >  1:569023/1‑130 (MQ=255)                                                                                                                              gtggtTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTAtt       <  2:6863/140‑1 (MQ=255)                                                                                                                              gtggtTGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTAtt       >  2:919328/1‑140 (MQ=255)                                                                                                                                   tGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAgcg  <  2:1071536/140‑1 (MQ=255)                                                                                                                                   tGAGAGCGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAgcg  <  1:49451/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCG‑TTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCG  >  NC_000913/548568‑548837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑