Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	557,620	A→G	V41A (GTG→GCG)	folD ←	bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase/ 5,10‑methylene‑tetrahydrofolate cyclohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	557,620	0	A	G	100.0%	214.8 / NA	57	V41A (GTG→GCG)	folD	bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase/ 5,10‑methylene‑tetrahydrofolate cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/29);  total (28/29)

TATCGATAAGCTCCAGCAGCTCCGCTTCGCTGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCAGAGAGAGGATTCC  >  NC_000913/557489‑557756                                                                                                                                    |                                                                                                                                         tATCGATAAGCTCCAGCAGCTCCGCTTCGCTGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGcaca                                                                                                                                  <  1:53221/140‑1 (MQ=255)         aGCTCCAGCAGCTCCGCTTCGCTGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAg                                                                                                                          >  1:982449/1‑140 (MQ=255)              cagcagCTCCGCTTCGCTGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTg                                                                                                                     >  1:751742/1‑140 (MQ=255)                          ttCGCTGGTGCTTTCGGGGAGGTAATAAGAGCGGGAGACAAACCCGACTTCTCCACAAGCCTTGCTTTTGCTGGCAAAATAAATTGGCGATCCGGGTTTACTCCCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTc                                                                                                         <  2:32970/140‑1 (MQ=255)                            cGCTGGTGGTTCCGGGGAGGTCATAAGGGGGGGAAACGACCCCGCTTTCTCCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGTTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCg                                                                                                       <  2:932049/140‑1 (MQ=255)                                ggtggtTTCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTg                                                                                                   <  1:887005/140‑1 (MQ=255)                                       tCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATAcg                                                                                            <  1:909879/140‑1 (MQ=255)                                         cGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgcg                                                                                          >  1:405705/1‑140 (MQ=255)                                            gAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCt                                                                                       >  1:91030/1‑140 (MQ=255)                                              ggTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGa                                                                                     >  1:1077220/1‑140 (MQ=255)                                              ggTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGa                                                                                     >  2:680520/1‑140 (MQ=255)                                                  aTAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtt                                                                                 >  1:757195/1‑140 (MQ=255)                                                    aaGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtttt                                                                               <  1:92562/140‑1 (MQ=255)                                                        gCGGGAGAGGACCCCGATTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGTTTCTTCCCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAg                                                                           <  2:504668/140‑1 (MQ=255)                                                         ggggAGACGACCCCGACTTCTTCACAAGCCTTGCTTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTCCCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGc                                                                          <  2:91030/139‑1 (MQ=255)                                                         cGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAGCTTTTTGAGc                                                                          >  1:777502/1‑140 (MQ=255)                                                         cGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAg                                                                           >  1:365255/1‑139 (MQ=255)                                                         cGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAg                                                                           <  2:365255/139‑1 (MQ=255)                                                         cGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGc                                                                          >  2:517781/1‑140 (MQ=255)                                                         cGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGc                                                                          >  2:343721/1‑140 (MQ=255)                                                          gggAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCa                                                                         >  2:88110/1‑140 (MQ=255)                                                          gggAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCa                                                                         >  2:124173/1‑140 (MQ=255)                                                              gaCGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt                                                                     >  1:956360/1‑140 (MQ=255)                                                                            cttcACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACctgc                                                       >  2:882829/1‑140 (MQ=255)                                                                                 caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCGGGGTTACTCCCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTgcgc                                                  <  2:381954/140‑1 (MQ=255)                                                                                 caACCCTTGCTTTGGCTTGCGACATAATTTTGCGATGCAGGTTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTgcgc                                                  <  2:405705/140‑1 (MQ=255)                                                                                 aaaGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCGGGTTTACTCCCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTgcgc                                                  <  2:982406/139‑1 (MQ=255)                                                                                       ttGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCaa                                                <  2:247930/136‑1 (MQ=255)                                                                                       ttGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCaa                                                >  1:247930/1‑136 (MQ=255)                                                                                       ttGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGt                                            >  2:8886/1‑140 (MQ=255)                                                                                       ttGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGt                                            >  2:475464/1‑140 (MQ=255)                                                                                        tGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACACGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGtt                                           >  1:595960/1‑140 (MQ=255)                                                                                            tttGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTAc                                       <  1:8886/140‑1 (MQ=255)                                                                                            tttGCTTGCGACAAAATTTGGCGATGCGGGGTTACTCCCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTAc                                       <  2:1077220/140‑1 (MQ=255)                                                                                            tttGCTTCCGACATAAATTTGCGATGCAGGGTTCCTCCCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTAc                                       <  2:70367/140‑1 (MQ=255)                                                                                             ttgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTAcc                                      <  1:680520/140‑1 (MQ=255)                                                                                              tgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCg                                     >  2:738859/1‑140 (MQ=255)                                                                                                cttgcGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTc                                   >  1:1119111/1‑140 (MQ=255)                                                                                                  tgcGACATAAATTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCaa                                 <  1:343721/140‑1 (MQ=255)                                                                                                            aTTTGCGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTtgc                       <  1:475464/140‑1 (MQ=255)                                                                                                                 cGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTtgc                       >  2:615765/1‑135 (MQ=255)                                                                                                                 cGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTtgc                       <  1:615765/135‑1 (MQ=255)                                                                                                                 cGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCa                  <  2:1033332/140‑1 (MQ=255)                                                                                                                 cGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCa                  >  1:1033332/1‑140 (MQ=255)                                                                                                                 cGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCa                  >  2:500063/1‑140 (MQ=255)                                                                                                                 cGATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCa                  >  1:583153/1‑140 (MQ=255)                                                                                                                  gATGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCAt                 <  2:757195/140‑1 (MQ=255)                                                                                                                   aTGCAGGGTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATc                >  1:254641/1‑140 (MQ=255)                                                                                                                         gtttACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCagagag          <  2:240049/138‑1 (MQ=255)                                                                                                                         ggTTACTCCCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCagagag          <  2:15804/140‑1 (MQ=255)                                                                                                                         ggTTACTACCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCagagag          <  2:751742/140‑1 (MQ=255)                                                                                                                              ctacCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTtgct                      >  2:981432/1‑123 (MQ=255)                                                                                                                              ctacCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTtgct                      <  1:981432/123‑1 (MQ=255)                                                                                                                              ctacCCGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCAGAGAGAGGAt     >  1:1125664/1‑140 (MQ=255)                                                                                                                                 cccGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCAGAGAGAGGATTcc  <  2:583153/140‑1 (MQ=255)                                                                                                                                 cccGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCAGAGAGAGGATTcc  <  1:124173/140‑1 (MQ=255)                                                                                                                                 cccGCCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACACGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCAGAGAGAGGATTcc  <  2:595960/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         TATCGATAAGCTCCAGCAGCTCCGCTTCGCTGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCAGAGAGAGGATTCC  >  NC_000913/557489‑557756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑