Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	560,634	T→C	V313A (GTT→GCT)	sfmD →	putative fimbrial usher protein SfmD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	560,634	0	T	C	100.0%	281.8 / NA	75	V313A (GTT→GCT)	sfmD	putative fimbrial usher protein SfmD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/38);  total (37/38)

TGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGTTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAATTCCGTATTCAACAGTGCCAATTTTACAACGC  >  NC_000913/560500‑560770                                                                                                                                       |                                                                                                                                         tgtACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTccc                                                                                                                                     >  1:332458/1‑140 (MQ=255)  gtACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTcccc                                                                                                                                    >  2:217733/1‑140 (MQ=255)     cGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCgg                                                                                                                                 <  2:451710/140‑1 (MQ=255)       gCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGgcg                                                                                                                               <  1:231485/140‑1 (MQ=255)           tATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCttt                                                                                                                           >  2:240582/1‑140 (MQ=255)           tATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCttt                                                                                                                           >  2:307613/1‑140 (MQ=255)                  ctGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAAtt                                                                                                                    >  1:780944/1‑140 (MQ=255)                              aTCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCAc                                                                                                        <  1:217733/140‑1 (MQ=255)                               tCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCAcc                                                                                                       >  2:857654/1‑140 (MQ=255)                               tCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCAcc                                                                                                       >  2:475527/1‑140 (MQ=255)                               tCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCAcc                                                                                                       >  2:53817/1‑140 (MQ=255)                               tCCTGATAGCCAGCAAGGGCTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCAcc                                                                                                       >  1:426116/1‑140 (MQ=255)                                    aTAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACa                                                                                                  <  2:426116/140‑1 (MQ=255)                                    aTAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACa                                                                                                  >  2:311501/1‑140 (MQ=255)                                    aTAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACa                                                                                                  >  1:348272/1‑140 (MQ=255)                                    aTAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACa                                                                                                  >  1:744903/1‑140 (MQ=255)                                    aTAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACa                                                                                                  >  2:521870/1‑140 (MQ=255)                                    aTAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACa                                                                                                  >  2:123991/1‑140 (MQ=255)                                    aTAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGA‑TGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTg                                                                                                           <  1:301323/130‑1 (MQ=255)                                    aTAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGA‑TGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTg                                                                                                           >  2:301323/1‑130 (MQ=255)                                       gCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCt                                                                                               >  2:134508/1‑140 (MQ=255)                                          aGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCa                                                                                            >  2:503353/1‑140 (MQ=255)                                          aGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCa                                                                                            >  1:206124/1‑140 (MQ=255)                                          aGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCa                                                                                            >  2:159233/1‑140 (MQ=255)                                          aGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCa                                                                                            <  2:348272/140‑1 (MQ=255)                                          aGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCa                                                                                            >  2:1020664/1‑140 (MQ=255)                                           gCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCaa                                                                                           <  1:292308/140‑1 (MQ=255)                                            cAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGa                                                                                                    <  2:637647/130‑1 (MQ=255)                                            cAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGa                                                                                                    >  1:637647/1‑130 (MQ=255)                                            cAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCaaa                                                                                          >  1:836468/1‑140 (MQ=255)                                               gggTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATgg                                                                                       <  1:983379/140‑1 (MQ=255)                                                    tGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATc                                                                                  <  1:173859/140‑1 (MQ=255)                                                      ccccAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTg                                                                                <  1:503353/140‑1 (MQ=255)                                                         cAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGAc                                                                             <  1:240582/140‑1 (MQ=255)                                                         cAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGAc                                                                             <  1:134508/140‑1 (MQ=255)                                                          aaCGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACg                                                                            <  1:307613/140‑1 (MQ=255)                                                                 cGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCAt                                                                     <  1:774417/140‑1 (MQ=255)                                                                       aTTGCCCTTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATcgacga                                                               >  2:841573/1‑140 (MQ=255)                                                                       aTTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATcgacga                                                               >  1:557918/1‑140 (MQ=255)                                                                       aTTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATcgacga                                                               >  1:549059/1‑140 (MQ=255)                                                                        ttGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAg                                                              <  2:836468/140‑1 (MQ=255)                                                                        ttGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAg                                                              <  1:521870/140‑1 (MQ=255)                                                                        ttGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAg                                                              <  1:475527/140‑1 (MQ=255)                                                                         tGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAgc                                                             >  1:1107498/1‑140 (MQ=255)                                                                             cGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGa                                                         <  1:1020664/140‑1 (MQ=255)                                                                             cGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGa                                                         <  1:159233/140‑1 (MQ=255)                                                                                aCGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATgg                                                      >  2:339831/1‑140 (MQ=255)                                                                                aCGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATgg                                                      >  1:897337/1‑140 (MQ=255)                                                                                  ggcggcCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCa                                                    <  2:31531/140‑1 (MQ=255)                                                                                  ggcggcCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCa                                                    <  2:780944/140‑1 (MQ=255)                                                                                  ggcggcCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCa                                                    <  1:587964/140‑1 (MQ=255)                                                                                    cggcCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGa                                                         <  1:512319/133‑1 (MQ=255)                                                                                    cggcCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGa                                                         >  2:512319/1‑133 (MQ=255)                                                                                     ggcCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATc                                                 <  2:557918/140‑1 (MQ=255)                                                                                       cccAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATcag                                               <  2:1107498/140‑1 (MQ=255)                                                                                            cTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGACATTACAGATTTGCGCCCGACATCTTCAAATGGCGATCTGGACGTCCCCATCGACGAGCGCGATGGCAATCAGCAGaa                                          <  2:685434/140‑1 (MQ=255)                                                                                            cTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGaa                                          <  2:332458/140‑1 (MQ=255)                                                                                               aCGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTa                                       <  1:707289/140‑1 (MQ=255)                                                                                                   ttCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTAcaca                                   <  2:897337/140‑1 (MQ=255)                                                                                                   ttCGGCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTAcaca                                   <  2:429510/140‑1 (MQ=255)                                                                                                      ggCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATcag                                               <  1:635212/125‑1 (MQ=255)                                                                                                      ggCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATcag                                               >  2:635212/1‑125 (MQ=255)                                                                                                      ggCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAAtt                                <  2:495969/140‑1 (MQ=255)                                                                                                      ggCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAAtt                                <  1:53817/140‑1 (MQ=255)                                                                                                      ggCAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAAtt                                <  1:857654/140‑1 (MQ=255)                                                                                                        cAAAATGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAATTcc                              <  2:206124/140‑1 (MQ=255)                                                                                                             tGGTTTTATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAATTCCGTAtt                         >  2:103700/1‑140 (MQ=255)                                                                                                                ttttATTATCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACGCAATTCCGTATTCaa                      <  1:123991/140‑1 (MQ=255)                                                                                                                    attatCTATCAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAATTCCGTATTCAACAGt                  <  2:744903/140‑1 (MQ=255)                                                                                                                       atctatcAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAATTCCGTATTCAACAGTGcc               >  2:819117/1‑140 (MQ=255)                                                                                                                       atctatcAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAATTCCGTATTCAACAGTGcc               >  1:589727/1‑140 (MQ=255)                                                                                                                         ctatcAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAATTCCGTATTCAACAGTGCCaa             >  2:124327/1‑140 (MQ=255)                                                                                                                          tatcAAAGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACAAGCGCGATGGCAATCAGCAGAATTACACAATTCCGTATTCAACAGTGCCAAt            >  2:462257/1‑140 (MQ=255)                                                                                                                                aGCTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAATTCCGTATTCAACAGTGCCAATTTTACa      <  2:549059/140‑1 (MQ=255)                                                                                                                                  cTATGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAATTCCGTATTCAACAGTGCCAATTTTACAAc    >  2:719203/1‑140 (MQ=255)                                                                                                                                    aTGCTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAATTCCGTATTCAACAGTGCCAATTTTACAAcgc  >  2:796005/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         TGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGTTTCCCCCGGCGCTTTTGAAATTACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAGCGCGATGGCAATCAGCAGAATTACACAATTCCGTATTCAACAGTGCCAATTTTACAACGC  >  NC_000913/560500‑560770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑