Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	616,675	A→G	D840G (GAC→GGC)	entF →	apo‑serine activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	616,675	0	A	G	100.0%	173.0 / NA	48	D840G (GAC→GGC)	entF	apo‑serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/19);  total (29/19)

CCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAGGCGCTGCCGGATGTCGAACAAGCC  >  NC_000913/616539‑616811                                                                                                                                         |                                                                                                                                         ccgccgGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGt                                                                                                                                       <  2:1095604/140‑1 (MQ=255)       ggTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCg                                                                                                                                 <  1:837347/140‑1 (MQ=255)           tgGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCgctgg                                                                                                                             <  1:849002/140‑1 (MQ=255)                     tctcTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCg                                                                                                                                 >  1:1050730/1‑126 (MQ=255)                     tctcTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCg                                                                                                                                 <  2:1050730/126‑1 (MQ=255)                        ctATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGgcg                                                                                                                <  2:803829/140‑1 (MQ=255)                        ctATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGgcg                                                                                                                <  1:453502/140‑1 (MQ=255)                        ctATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGgcg                                                                                                                <  1:753777/140‑1 (MQ=255)                              cACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTgg                                                                                                          >  1:1107006/1‑140 (MQ=255)                               aCTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGa                                                                                                         >  2:56382/1‑140 (MQ=255)                               aCTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGa                                                                                                         >  2:291283/1‑140 (MQ=255)                               aCTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGa                                                                                                         >  2:1043882/1‑140 (MQ=255)                               aCTGGCATTCAACTGGCGCA‑GGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAg                                                                                                        >  1:1091167/1‑140 (MQ=255)                                cTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAg                                                                                                        >  2:867074/1‑140 (MQ=255)                                cTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTCGCTGGATAACGGCGCGGTGGAg                                                                                                        >  1:897996/1‑140 (MQ=255)                                 tGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGt                                                                                                       >  1:794055/1‑140 (MQ=255)                                   gCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTAc                                                                                                     >  1:691089/1‑140 (MQ=255)                                   gCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTAc                                                                                                     >  1:553871/1‑140 (MQ=255)                                   gCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTAc                                                                                                     <  2:1107006/140‑1 (MQ=255)                                      ttCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTc                                                                                                  >  1:680923/1‑140 (MQ=255)                                         aaCTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCggg                                                                                               <  1:484133/140‑1 (MQ=255)                                         aaCTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCggg                                                                                               <  2:897996/140‑1 (MQ=255)                                          aCTGGCGCA‑GGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCGTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGgcg                                                                                             <  2:1091167/140‑1 (MQ=255)                                             ggCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTTCCTCGGgcg                                                                                             <  2:407813/138‑1 (MQ=255)                                             ggCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCggg                                                                                               <  1:767084/136‑1 (MQ=255)                                             ggCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCggg                                                                                               >  2:767084/1‑136 (MQ=255)                                             ggCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGgcg                                                                                             >  1:407813/1‑138 (MQ=255)                                                    ggCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGtgatga                                                                                    >  2:968472/1‑140 (MQ=255)                                                         tctcGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGc                                                                               >  2:34417/1‑140 (MQ=255)                                                                   cccGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTcg                                                                     >  1:441332/1‑140 (MQ=255)                                                                    ccGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTcgc                                                                    >  1:1008008/1‑140 (MQ=255)                                                                         cTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCa                                                               <  1:979223/140‑1 (MQ=255)                                                                           gACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGc                                                             <  1:867074/140‑1 (MQ=255)                                                                             ccGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGt                                                           >  1:659343/1‑140 (MQ=255)                                                                                  aGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGa                                                      >  1:896026/1‑140 (MQ=255)                                                                                      gCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTg                                                  >  2:476388/1‑140 (MQ=255)                                                                                                 gATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAatcga                                       <  2:794055/140‑1 (MQ=255)                                                                                                      ttttGCCCCAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATcgcg                                  <  1:1135960/140‑1 (MQ=255)                                                                                                           ccccAGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATg                             >  2:987142/1‑140 (MQ=255)                                                                                                               aGGTGAACGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAgg                         >  1:285315/1‑140 (MQ=255)                                                                                                                     aCGGATGTACCGTACCGGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAGGCGCTGc                   <  1:476388/140‑1 (MQ=255)                                                                                                                            taccgtaccgGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAGGCGCTGCCGGATGt            >  2:261151/1‑140 (MQ=255)                                                                                                                                 taccgGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAGGCGCTGCCGGATGTCGAAc       >  1:219175/1‑140 (MQ=255)                                                                                                                                   ccgGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAGGCGCTGCCGGATGTCGaa        >  1:175253/1‑137 (MQ=255)                                                                                                                                   ccgGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAGGCGCTGCCGGATGTCGaa        <  2:175253/137‑1 (MQ=255)                                                                                                                                   ccgGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAGGCGCTGCCGGATGTCGAACaa     >  2:236790/1‑140 (MQ=255)                                                                                                                                   ccgGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAGGCGCTGCCGGATGTCGAACaa     >  2:234515/1‑140 (MQ=255)                                                                                                                                   ccgGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAGGCGCTGCCGGATGTCGAACaa     >  2:871104/1‑140 (MQ=255)                                                                                                                                   ccgGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAGGCGCTGCCGGATGTCGAACaa     >  1:419512/1‑140 (MQ=255)                                                                                                                                    cgGAGGCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGTAGGCGCTGCCGGATGTCGAACAAg    <  2:553871/140‑1 (MQ=255)                                                                                                                                      gaggCGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAGGCGCTGCCGGATGTCGAACAAGcc  >  2:1083226/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         CCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTAAAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAGGCGCTGCCGGATGTCGAACAAGCC  >  NC_000913/616539‑616811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑