Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	682,783	T→C	E204G (GAA→GGA)	hscC ←	chaperone protein HscC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	682,783	0	T	C	100.0%	210.0 / NA	56	E204G (GAA→GGA)	hscC	chaperone protein HscC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/28);  total (28/28)

GTGCAATGGCGATTGATTGCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGC  >  NC_000913/682647‑682918                                                                                                                                         |                                                                                                                                        gtgCAATGGCGATTGATTGCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGc                                                                                                                                      <  1:978331/140‑1 (MQ=255)  tgCAATGGCGATTGATTGCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGcc                                                                                                                                     >  2:975382/1‑140 (MQ=255)     aaTGGCGATTGATTGCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGccacc                                                                                                                                  <  1:527320/140‑1 (MQ=255)         gCGATTGATTGCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGa                                                                                                                              <  2:318651/140‑1 (MQ=255)         gCGATTGATTGCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGa                                                                                                                              <  2:674524/140‑1 (MQ=255)          cGATTGATTGCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGaa                                                                                                                             >  1:325968/1‑140 (MQ=255)            attgattgCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAg                                                                                                                           >  1:378885/1‑140 (MQ=255)                   gCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGc                                                                                                                    >  2:486323/1‑140 (MQ=255)                   gCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGc                                                                                                                    <  2:827327/140‑1 (MQ=255)                    cTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGa                                                                                                                              >  1:1109954/1‑129 (MQ=255)                    cTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGa                                                                                                                              <  2:1109954/129‑1 (MQ=255)                          tttGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGa                                                                                                             >  2:4985/1‑140 (MQ=255)                              ccGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCg                                                                                                         <  1:666447/140‑1 (MQ=255)                               cGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGt                                                                                                        <  2:221028/140‑1 (MQ=255)                               cGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGt                                                                                                        >  2:885240/1‑140 (MQ=255)                                         caGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAAt                                                                                              <  1:4985/140‑1 (MQ=255)                                           ggCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCa                                                                                            <  1:885240/140‑1 (MQ=255)                                             cGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACt                                                                                                   >  2:820935/1‑131 (MQ=255)                                             cGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACt                                                                                                   <  1:820935/131‑1 (MQ=255)                                              gTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCg                                                                                         >  1:869591/1‑140 (MQ=255)                                               tACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCgg                                                                                        >  1:59774/1‑140 (MQ=255)                                               tACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCgg                                                                                        >  2:781509/1‑140 (MQ=255)                                                aCAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCAc                                                                                           >  2:252499/1‑136 (MQ=255)                                                aCAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCAc                                                                                           <  1:252499/136‑1 (MQ=255)                                                 cAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCggcg                                                                                      >  1:1129823/1‑140 (MQ=255)                                                 cAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCggcg                                                                                      <  2:1047838/140‑1 (MQ=255)                                                    ggCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCggcgtgg                                                                                   >  1:988165/1‑140 (MQ=255)                                                      cTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCggcgtggcg                                                                                 <  1:48787/140‑1 (MQ=255)                                                      cTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCggcgtggcg                                                                                 >  1:791112/1‑140 (MQ=255)                                                      cTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCggcgtggcg                                                                                 >  1:684926/1‑140 (MQ=255)                                                      cTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCggcg                                                                                      >  1:1042723/1‑135 (MQ=255)                                                      cTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCggcg                                                                                      <  2:1042723/135‑1 (MQ=255)                                                      cTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCggc                                                                                       >  1:216716/1‑134 (MQ=255)                                                      cTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCggc                                                                                       <  2:216716/134‑1 (MQ=255)                                                         ccAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTAc                                                                              <  1:537048/140‑1 (MQ=255)                                                             ttCACTCTCGTTAAGCGTGGTCCTGACGACATCCGCGCGCTTTAAAACCTCATCGACCAGCATATAGGTAAAATCTCCGCCATCAATCAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCaa                                                                          >  2:997071/1‑140 (MQ=255)                                                              tCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAg                                                                         <  2:325968/140‑1 (MQ=255)                                                                      gTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTaa                                                                 >  1:453788/1‑140 (MQ=255)                                                                                      gCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTgccgcc                                                 >  2:186164/1‑140 (MQ=255)                                                                                          cATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCg                                             <  2:59774/140‑1 (MQ=255)                                                                                           aTCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCga                                            >  2:950726/1‑140 (MQ=255)                                                                                                cgcgTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCAACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCa                                       >  2:878006/1‑140 (MQ=255)                                                                                                  cgTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCaaa                                     <  2:869591/140‑1 (MQ=255)                                                                                                        aagaCCGCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCTTAACGTGAAACGTGCCGCCACCGAGATCAAACACCAg                               <  2:132737/137‑1 (MQ=255)                                                                                                          aaCCTCATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCg                             <  2:378885/140‑1 (MQ=255)                                                                                                               cATCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACgg                        >  2:598329/1‑140 (MQ=255)                                                                                                                 tCGACCAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGt                      <  1:598329/140‑1 (MQ=255)                                                                                                                      cAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTAtttt                 <  1:781509/140‑1 (MQ=255)                                                                                                                      cAGCATATGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTAtttt                 <  2:988165/140‑1 (MQ=255)                                                                                                                          atatGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTttgttg             <  1:186164/140‑1 (MQ=255)                                                                                                                           tatGGGTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTgg            <  2:684926/140‑1 (MQ=255)                                                                                                                              gggTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTa         >  2:779414/1‑140 (MQ=255)                                                                                                                              gggTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTa         >  2:150982/1‑140 (MQ=255)                                                                                                                                gTAAAATCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATg       <  1:150982/140‑1 (MQ=255)                                                                                                                                   aaaTCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAg    >  2:592521/1‑140 (MQ=255)                                                                                                                                   aaaTCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAg    >  2:352673/1‑140 (MQ=255)                                                                                                                                     aTCTCCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGc  >  2:9592/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GTGCAATGGCGATTGATTGCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGC  >  NC_000913/682647‑682918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑