Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,331	T→C	intergenic (‑5/+43)	umpH ← / ← nagC	UMP phosphatase/DNA‑binding transcriptional dual regulator NagC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,331	0	T	C	100.0%	180.8 / NA	48	intergenic (‑5/+43)	umpH/nagC	UMP phosphatase/DNA‑binding transcriptional dual regulator NagC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/27);  total (21/27)

ATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCT  >  NC_000913/700195‑700465                                                                                                                                         |                                                                                                                                       aTAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGaccacc                                                                                                                                     >  2:421541/1‑140 (MQ=255) aTAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGaccacc                                                                                                                                     >  2:872703/1‑140 (MQ=255) aTAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGaccacc                                                                                                                                     >  1:831135/1‑140 (MQ=255) aTAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGaccacc                                                                                                                                     <  1:179912/140‑1 (MQ=255)  tAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCAccc                                                                                                                                    <  1:423784/140‑1 (MQ=255)     ttGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGa                                                                                                                                 >  1:826786/1‑140 (MQ=255)        gTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAata                                                                                                                              >  1:921712/1‑140 (MQ=255)        gTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAata                                                                                                                              >  2:1034537/1‑140 (MQ=255)          cAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATAtt                                                                                                                            >  1:602653/1‑140 (MQ=255)             cAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGac                                                                                                                         <  2:831135/140‑1 (MQ=255)             cAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGac                                                                                                                         <  2:602653/140‑1 (MQ=255)                cacCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGacaac                                                                                                                      >  1:523056/1‑140 (MQ=255)                      cGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAg                                                                                                                <  2:523056/140‑1 (MQ=255)                       ggCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAgc                                                                                                               <  1:872703/140‑1 (MQ=255)                        gCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACAGTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAgcg                                                                                                              >  2:565443/1‑140 (MQ=255)                         cAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAgcgc                                                                                                             <  1:1034537/140‑1 (MQ=255)                         cAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAgcgc                                                                                                             <  1:971464/140‑1 (MQ=255)                         cAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAgcgc                                                                                                             <  1:540993/140‑1 (MQ=255)                         cAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAgcgc                                                                                                             <  1:171689/140‑1 (MQ=255)                         cAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAgcgc                                                                                                             <  1:1048181/140‑1 (MQ=255)                          aGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGcc                                                                                                            <  1:421541/140‑1 (MQ=255)                          aGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGcc                                                                                                            <  1:410370/140‑1 (MQ=255)                           ggCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCa                                                                                                           <  2:471075/140‑1 (MQ=255)                                 tATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATaa                                                                                                     >  1:41046/1‑140 (MQ=255)                                        aaTCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGcaca                                                                                              >  1:568785/1‑140 (MQ=255)                                          tCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACAtt                                                                                            >  2:524807/1‑140 (MQ=255)                                             cGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAAt                                                                                         >  1:186497/1‑140 (MQ=255)                                             cGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAAt                                                                                         >  1:96773/1‑140 (MQ=255)                                               tGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAAttt                                                                                       >  2:733351/1‑140 (MQ=255)                                                   aaaaTTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCa                                                                                   <  2:568785/140‑1 (MQ=255)                                                           gCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGc                                                                           <  1:611658/140‑1 (MQ=255)                                                                aCCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAg                                                                      <  2:96773/140‑1 (MQ=255)                                                                aCCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCAATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAg                                                                      <  2:817419/140‑1 (MQ=255)                                                                  ccGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCa                                                                    <  2:96833/140‑1 (MQ=255)                                                                    ggTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCaaa                                                                  <  2:41046/140‑1 (MQ=255)                                                                     gTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCaaaa                                                                 <  2:826786/140‑1 (MQ=255)                                                                         ggCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATAcc                                                             >  2:514061/1‑140 (MQ=255)                                                                           cGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGt                                                           <  1:733351/140‑1 (MQ=255)                                                                           cGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGt                                                           >  2:177104/1‑140 (MQ=255)                                                                                    cGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATgg                                                  <  2:151379/140‑1 (MQ=255)                                                                                             gCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTa                                         >  2:172065/1‑140 (MQ=255)                                                                                                 gCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAg                                     >  1:1113294/1‑140 (MQ=255)                                                                                                  ccGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATgg                                                  <  2:140507/126‑1 (MQ=255)                                                                                                  ccGTCGATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATgg                                                  >  1:140507/1‑126 (MQ=255)                                                                                                       gATATCGCAAATTACATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCaaa                               >  2:229967/1‑140 (MQ=255)                                                                                                                     cATTTTTAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCg                 <  1:57901/140‑1 (MQ=255)                                                                                                                          ttAATGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCgg            <  1:177104/140‑1 (MQ=255)                                                                                                                            aaTGGTCATGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTg          >  1:831611/1‑140 (MQ=255)                                                                                                                                   aTGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGc   >  1:522185/1‑140 (MQ=255)                                                                                                                                    tGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCt  <  2:522185/140‑1 (MQ=255)                                                                                                                                    tGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCt  >  1:21650/1‑140 (MQ=255)                                                                                                                                    tGGACCACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGATGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCt  <  1:1036609/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       ATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCT  >  NC_000913/700195‑700465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑